Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds

S. E. Pen, C. F. Lo, S. C. Li, L. L. Che, Y. S. Chang, K. F. Liu, M. S. Su, G. H. Kou

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46 Citations (Scopus)

Abstract

In a survey of 27 Penaeus monodon culture ponds stocked with postlarvae (∼PL10) at medium density (∼40 shrimp m-2), single-step nested white spot syndrome virus (WSSV) PCR was used to measure the WSSV infection rates in the shrimp populations within 1 mo after stocking. Seven ponds were initially WSSV-free, and the shrimp in 5 of these were harvested successfully. In the ponds (n = 6) where detection rates were higher than 50%, mass mortality occurred during the growth period, and none of these ponds was harvested successfully. In a subsequent study, P. monodon brooders were classified into 3 groups according to their WSSV infection status before and after spawning: brooders that were WSSV-positive before spawning were assigned to group A; spawners that became WSSV-positive only after spawning were assigned to group B; and group C consisted of brooders that were still WSSV-negative after spawning. WSSV screening showed that 75, 44 and 14%, respectively, of group A, B and C brooders produced nauplii that were WSSV-positive. Most (57%; 16/28) of the brooders in group A produced nauplii in which the WSSV prevalence was high (>50%).When a pond was stocked with high-prevalence nauplii from 1 of these group A brooders, an outbreak of white spot syndrome occurred within 3 wk and only ∼20% of the initial population survived through to harvest (after 174 d). By contrast, 2 other ponds stocked with low-prevalence and WSSV-negative nauplii (derived respectively from 2 brooders in group B), both had much higher survival rates (70 to 80%) and yielded much larger (∼3× by weight) total harvests. We conclude that testing the nauplii is an effective and practical screening strategy for commercially cultured P. monodon.

Original languageEnglish
Pages (from-to)165-172
Number of pages8
JournalDiseases of Aquatic Organisms
Volume46
Issue number3
DOIs
Publication statusPublished - 2001 Oct 8

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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    Pen, S. E., Lo, C. F., Li, S. C., Che, L. L., Chang, Y. S., Liu, K. F., Su, M. S., & Kou, G. H. (2001). Performance of WSSV-infected and WSSV-negative Penaeus monodon postlarvae in culture ponds. Diseases of Aquatic Organisms, 46(3), 165-172. https://doi.org/10.3354/dao046165