Peripherin is tyrosine-phosphorylated at its carboxyl-terminal tyrosine

James M. Angelastro, Chung-Liang Ho, Thierry Frappier, Ronald K H Liem, Lloyd A. Greene

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Peripherin is a type III intermediate filament present in peripheral and certain CNS neurons. We report here that peripherin contains a phosphotyrosine residue and, as such, is the only identified intermediate filament protein known to be modified in this manner. Antiserum specific for phosphotyrosine recognizes peripherin present in PC12 cells (with or without nerve growth factor treatment) and in rat sciatic nerve as well as that expressed in Sf-9 cells and SW-13 cl. 2 vim- cells. The identity of peripherin as a tyrosine-phosphorylated protein in PC12 cells was confirmed by immunoprecipitation, two-dimensional isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels, and phosphoamino acid analysis. Unlike serine/threonine phosphorylation, tyrosine phosphorylation of peripherin is not regulated by depolarization or nerve growth factor treatment. To identify the site of tyrosine phosphorylation, rat peripherin was mutated at several tyrosine residues and expressed in SW-13 cl. 2 vim- cells. Tyrosine phosphorylation was selectively lost only for peripherin mutants in which the carboxy-terminal tyrosine (Y474) was mutated. Indirect immunofluorescence staining indicated that both wild-type peripherin and peripherin Y474F form a filamentous network in SW-13 cl. 2 vim- cells. This indicates that tyrosine phosphorylation of the peripherin C-terminal residue is not required for assembly and leaves open the possibility that this modification serves other functions.

Original languageEnglish
Pages (from-to)540-549
Number of pages10
JournalJournal of Neurochemistry
Volume70
Issue number2
Publication statusPublished - 1998 Feb

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Peripherins
Tyrosine
Phosphorylation
Phosphotyrosine
PC12 Cells
Nerve Growth Factor
Rats
Phosphoamino Acids
Intermediate Filament Proteins
Intermediate Filaments
Depolarization
Isoelectric Focusing
Sciatic Nerve
Threonine
Indirect Fluorescent Antibody Technique
Electrophoresis
Immunoprecipitation
Sodium Dodecyl Sulfate
Serine
Neurons

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Angelastro, J. M., Ho, C-L., Frappier, T., Liem, R. K. H., & Greene, L. A. (1998). Peripherin is tyrosine-phosphorylated at its carboxyl-terminal tyrosine. Journal of Neurochemistry, 70(2), 540-549.
Angelastro, James M. ; Ho, Chung-Liang ; Frappier, Thierry ; Liem, Ronald K H ; Greene, Lloyd A. / Peripherin is tyrosine-phosphorylated at its carboxyl-terminal tyrosine. In: Journal of Neurochemistry. 1998 ; Vol. 70, No. 2. pp. 540-549.
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Angelastro, JM, Ho, C-L, Frappier, T, Liem, RKH & Greene, LA 1998, 'Peripherin is tyrosine-phosphorylated at its carboxyl-terminal tyrosine', Journal of Neurochemistry, vol. 70, no. 2, pp. 540-549.

Peripherin is tyrosine-phosphorylated at its carboxyl-terminal tyrosine. / Angelastro, James M.; Ho, Chung-Liang; Frappier, Thierry; Liem, Ronald K H; Greene, Lloyd A.

In: Journal of Neurochemistry, Vol. 70, No. 2, 02.1998, p. 540-549.

Research output: Contribution to journalArticle

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Angelastro JM, Ho C-L, Frappier T, Liem RKH, Greene LA. Peripherin is tyrosine-phosphorylated at its carboxyl-terminal tyrosine. Journal of Neurochemistry. 1998 Feb;70(2):540-549.