Peroxide responsive regulator PerR of group A streptococcus is required for the expression of phage-associated DNase Sda1 under oxidative stress

Chih Hung Wang, Chuan Chiang-Ni, Hsin Tzu Kuo, Po Xing Zheng, Chih Cheng Tsou, Shuying Wang, Pei Jane Tsai, Woei Jer Chuang, Yee Shin Lin, Ching Chuan Liu, Jiunn Jong Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The peroxide regulator (PerR) is a ferric uptake repressor-like protein, which is involved in adaptation to oxidative stress and iron homeostasis in group A streptococcus. A perR mutant is attenuated in surviving in human blood, colonization of the pharynx, and resistance to phagocytic clearance, indicating that the PerR regulon affects both host environment adaptation and immune escape. Sda1 is a phage-associated DNase which promotes M1T1 group A streptococcus escaping from phagocytic cells by degrading DNA-based neutrophil extracellular traps. In the present study, we found that the expression of sda1 is up-regulated under oxidative conditions in the wild-type strain but not in the perR mutant. A gel mobility shift assay showed that the recombinant PerR protein binds the sda1 promoter. In addition, mutation of the conserved histidine residue in the metal binding site of PerR abolished sda1 expression under hydrogen peroxide treatment conditions, suggesting that PerR is directly responsible for the sda1 expression under oxidative stress. Our results reveal PerR-dependent sda1 expression under oxidative stress, which may aid innate immune escape of group A streptococcus.

Original languageEnglish
Article numbere81882
JournalPloS one
Volume8
Issue number12
DOIs
Publication statusPublished - 2013 Dec 3

Fingerprint

deoxyribonucleases
Bacteriophages
Oxidative stress
Deoxyribonucleases
Streptococcus
Peroxides
peroxides
bacteriophages
Oxidative Stress
oxidative stress
Repressor Proteins
Regulon
regulon
mutants
pharynx
phagocytes
Electrophoretic Mobility Shift Assay
Phagocytes
Pharynx
histidine

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

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title = "Peroxide responsive regulator PerR of group A streptococcus is required for the expression of phage-associated DNase Sda1 under oxidative stress",
abstract = "The peroxide regulator (PerR) is a ferric uptake repressor-like protein, which is involved in adaptation to oxidative stress and iron homeostasis in group A streptococcus. A perR mutant is attenuated in surviving in human blood, colonization of the pharynx, and resistance to phagocytic clearance, indicating that the PerR regulon affects both host environment adaptation and immune escape. Sda1 is a phage-associated DNase which promotes M1T1 group A streptococcus escaping from phagocytic cells by degrading DNA-based neutrophil extracellular traps. In the present study, we found that the expression of sda1 is up-regulated under oxidative conditions in the wild-type strain but not in the perR mutant. A gel mobility shift assay showed that the recombinant PerR protein binds the sda1 promoter. In addition, mutation of the conserved histidine residue in the metal binding site of PerR abolished sda1 expression under hydrogen peroxide treatment conditions, suggesting that PerR is directly responsible for the sda1 expression under oxidative stress. Our results reveal PerR-dependent sda1 expression under oxidative stress, which may aid innate immune escape of group A streptococcus.",
author = "Wang, {Chih Hung} and Chuan Chiang-Ni and Kuo, {Hsin Tzu} and Zheng, {Po Xing} and Tsou, {Chih Cheng} and Shuying Wang and Tsai, {Pei Jane} and Chuang, {Woei Jer} and Lin, {Yee Shin} and Liu, {Ching Chuan} and Wu, {Jiunn Jong}",
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Peroxide responsive regulator PerR of group A streptococcus is required for the expression of phage-associated DNase Sda1 under oxidative stress. / Wang, Chih Hung; Chiang-Ni, Chuan; Kuo, Hsin Tzu; Zheng, Po Xing; Tsou, Chih Cheng; Wang, Shuying; Tsai, Pei Jane; Chuang, Woei Jer; Lin, Yee Shin; Liu, Ching Chuan; Wu, Jiunn Jong.

In: PloS one, Vol. 8, No. 12, e81882, 03.12.2013.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Peroxide responsive regulator PerR of group A streptococcus is required for the expression of phage-associated DNase Sda1 under oxidative stress

AU - Wang, Chih Hung

AU - Chiang-Ni, Chuan

AU - Kuo, Hsin Tzu

AU - Zheng, Po Xing

AU - Tsou, Chih Cheng

AU - Wang, Shuying

AU - Tsai, Pei Jane

AU - Chuang, Woei Jer

AU - Lin, Yee Shin

AU - Liu, Ching Chuan

AU - Wu, Jiunn Jong

PY - 2013/12/3

Y1 - 2013/12/3

N2 - The peroxide regulator (PerR) is a ferric uptake repressor-like protein, which is involved in adaptation to oxidative stress and iron homeostasis in group A streptococcus. A perR mutant is attenuated in surviving in human blood, colonization of the pharynx, and resistance to phagocytic clearance, indicating that the PerR regulon affects both host environment adaptation and immune escape. Sda1 is a phage-associated DNase which promotes M1T1 group A streptococcus escaping from phagocytic cells by degrading DNA-based neutrophil extracellular traps. In the present study, we found that the expression of sda1 is up-regulated under oxidative conditions in the wild-type strain but not in the perR mutant. A gel mobility shift assay showed that the recombinant PerR protein binds the sda1 promoter. In addition, mutation of the conserved histidine residue in the metal binding site of PerR abolished sda1 expression under hydrogen peroxide treatment conditions, suggesting that PerR is directly responsible for the sda1 expression under oxidative stress. Our results reveal PerR-dependent sda1 expression under oxidative stress, which may aid innate immune escape of group A streptococcus.

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