Phenotypic analysis of human placenta-derived mesenchymal stem cells

Ying Hui A. Chen, Abby H.C. Lin, Chi Mou Liu, Chiung-Hsin Chang, Chen-Hsiang Yu, Lynn Ling-Huei Huang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Mesenchymal stem cells are promising tools for clinical application in regenerative medicine due to their unique properties. Owing to their plasticity, mesenchymal stem cells isolated from human placental tissues have become a useful alternative to those derived from bone marrow. Although the multipotency of these placenta-derived mesenchymal stem cells is comparable to that of bone marrow-derived cells, those from different placental compartments display variable plasticity. Detailed phenotypic analyses are required to fully characterize stem cell populations isolated from distinct tissue sources. Here, we characterized a population of embryonic stem cell-like progenitor cells from placental chorionic villi. In addition to fulfilling the criteria for mesenchymal stem cells, these also expressed several embryonic stem cell biomarkers, including Oct-3/4, Sox-2, and TRA-1-81. Expression of these biomarkers was consistent among different colonies and increased through culture with the exception for ATP-binding cassette sub-family G member 2 (ABCG2). To determine whether ABCG2 represented a marker for cells with embryonic stem cell-like properties, we analyzed ABCG2+ and ABCG2colonies and determined the differentiation potential of populations expanded from these colonies; however, no significant differences were observed. Thus, multipotent mesenchymal stem cells from human placental chorionic villi possess embryonic stem cell-like features and could be clinically useful.

Original languageEnglish
Pages (from-to)188-202
Number of pages15
JournalJournal of Biomaterials and Tissue Engineering
Volume7
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

Fingerprint

Chorionic Villi
Stem cells
Mesenchymal Stromal Cells
Placenta
Embryonic Stem Cells
Adenosine Triphosphate
Adenosinetriphosphate
Stem Cells
Biomarkers
Multipotent Stem Cells
Population
Regenerative Medicine
Bone Marrow Cells
Plasticity
Bone
Bone Marrow
Tissue

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

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title = "Phenotypic analysis of human placenta-derived mesenchymal stem cells",
abstract = "Mesenchymal stem cells are promising tools for clinical application in regenerative medicine due to their unique properties. Owing to their plasticity, mesenchymal stem cells isolated from human placental tissues have become a useful alternative to those derived from bone marrow. Although the multipotency of these placenta-derived mesenchymal stem cells is comparable to that of bone marrow-derived cells, those from different placental compartments display variable plasticity. Detailed phenotypic analyses are required to fully characterize stem cell populations isolated from distinct tissue sources. Here, we characterized a population of embryonic stem cell-like progenitor cells from placental chorionic villi. In addition to fulfilling the criteria for mesenchymal stem cells, these also expressed several embryonic stem cell biomarkers, including Oct-3/4, Sox-2, and TRA-1-81. Expression of these biomarkers was consistent among different colonies and increased through culture with the exception for ATP-binding cassette sub-family G member 2 (ABCG2). To determine whether ABCG2 represented a marker for cells with embryonic stem cell-like properties, we analyzed ABCG2+ and ABCG2−colonies and determined the differentiation potential of populations expanded from these colonies; however, no significant differences were observed. Thus, multipotent mesenchymal stem cells from human placental chorionic villi possess embryonic stem cell-like features and could be clinically useful.",
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Phenotypic analysis of human placenta-derived mesenchymal stem cells. / Chen, Ying Hui A.; Lin, Abby H.C.; Liu, Chi Mou; Chang, Chiung-Hsin; Yu, Chen-Hsiang; Huang, Lynn Ling-Huei.

In: Journal of Biomaterials and Tissue Engineering, Vol. 7, No. 3, 01.03.2017, p. 188-202.

Research output: Contribution to journalArticle

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