Phenotypic and functional analysis of natural killer cells in systemic lupus erythematosus patients.

B. L. Chiang, Y. C. Chen, C. R. Wang, Y. L. Lin, C. C. Chou, K. H. Hsieh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Systemic lupus erythematosus (SLE) manifested with multiple autoantibodies production and glomerulonephritis, is the best example of systemic autoimmune diseases. To further elucidate the role of cytokines and the potential involvement of natural killer cells (NK cells) in the pathogenesis of lupus, phenotypic analysis of peripheral blood mononuclear cells (PBMC), NK cells cytotoxicity and cytokines production pattern of SLE patients and normal controls were examined. In addition, the effect of a variety of cytokines on anti-dsDNA antibodies production was also investigated. Our results showed that: (a) there was an increased percentage of memory T cells and decreased percentage of NK cells in SLE patients when compared to normal controls (p < 0.05); (b) a decreased production of cytokines like gamma-IFN in mitogen-stimulated PBMCs was also noted in SLE patients; (c) cytolytic activity of NK cells was markedly reduced in SLE patients (p < 0.05); (d) spontaneous secretion of IgG anti-dsDNA antibodies by B cells isolated from SLE patients could be inhibited by gamma-IFN, but not by IL-2, IL-4 and IL-5. These data suggested that decreased functions of NK cells and related type 1 T helper cells be closely related to the immune dysregulation and autoantibodies production in SLE.

Original languageEnglish
Pages (from-to)357-363
Number of pages7
JournalZhonghua Minguo xiao er ke yi xue hui za zhi [Journal]. Zhonghua Minguo xiao er ke yi xue hui
Volume36
Issue number5
Publication statusPublished - 1995 Jan 1

Fingerprint

Natural Killer Cells
Systemic Lupus Erythematosus
Cytokines
Autoantibodies
Anti-Idiotypic Antibodies
Th1 Cells
Interleukin-5
Glomerulonephritis
Mitogens
Interleukin-4
Autoimmune Diseases
Antibody Formation
Interleukin-2
Blood Cells
B-Lymphocytes
Immunoglobulin G
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Pediatrics, Perinatology, and Child Health

Cite this

@article{1817169f6a544dfea02bbf2b5ee17a08,
title = "Phenotypic and functional analysis of natural killer cells in systemic lupus erythematosus patients.",
abstract = "Systemic lupus erythematosus (SLE) manifested with multiple autoantibodies production and glomerulonephritis, is the best example of systemic autoimmune diseases. To further elucidate the role of cytokines and the potential involvement of natural killer cells (NK cells) in the pathogenesis of lupus, phenotypic analysis of peripheral blood mononuclear cells (PBMC), NK cells cytotoxicity and cytokines production pattern of SLE patients and normal controls were examined. In addition, the effect of a variety of cytokines on anti-dsDNA antibodies production was also investigated. Our results showed that: (a) there was an increased percentage of memory T cells and decreased percentage of NK cells in SLE patients when compared to normal controls (p < 0.05); (b) a decreased production of cytokines like gamma-IFN in mitogen-stimulated PBMCs was also noted in SLE patients; (c) cytolytic activity of NK cells was markedly reduced in SLE patients (p < 0.05); (d) spontaneous secretion of IgG anti-dsDNA antibodies by B cells isolated from SLE patients could be inhibited by gamma-IFN, but not by IL-2, IL-4 and IL-5. These data suggested that decreased functions of NK cells and related type 1 T helper cells be closely related to the immune dysregulation and autoantibodies production in SLE.",
author = "Chiang, {B. L.} and Chen, {Y. C.} and Wang, {C. R.} and Lin, {Y. L.} and Chou, {C. C.} and Hsieh, {K. H.}",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "36",
pages = "357--363",
journal = "Pediatrics and Neonatology",
issn = "1875-9572",
publisher = "Elsevier (Singapore) Pte Ltd",
number = "5",

}

Phenotypic and functional analysis of natural killer cells in systemic lupus erythematosus patients. / Chiang, B. L.; Chen, Y. C.; Wang, C. R.; Lin, Y. L.; Chou, C. C.; Hsieh, K. H.

In: Zhonghua Minguo xiao er ke yi xue hui za zhi [Journal]. Zhonghua Minguo xiao er ke yi xue hui, Vol. 36, No. 5, 01.01.1995, p. 357-363.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Phenotypic and functional analysis of natural killer cells in systemic lupus erythematosus patients.

AU - Chiang, B. L.

AU - Chen, Y. C.

AU - Wang, C. R.

AU - Lin, Y. L.

AU - Chou, C. C.

AU - Hsieh, K. H.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Systemic lupus erythematosus (SLE) manifested with multiple autoantibodies production and glomerulonephritis, is the best example of systemic autoimmune diseases. To further elucidate the role of cytokines and the potential involvement of natural killer cells (NK cells) in the pathogenesis of lupus, phenotypic analysis of peripheral blood mononuclear cells (PBMC), NK cells cytotoxicity and cytokines production pattern of SLE patients and normal controls were examined. In addition, the effect of a variety of cytokines on anti-dsDNA antibodies production was also investigated. Our results showed that: (a) there was an increased percentage of memory T cells and decreased percentage of NK cells in SLE patients when compared to normal controls (p < 0.05); (b) a decreased production of cytokines like gamma-IFN in mitogen-stimulated PBMCs was also noted in SLE patients; (c) cytolytic activity of NK cells was markedly reduced in SLE patients (p < 0.05); (d) spontaneous secretion of IgG anti-dsDNA antibodies by B cells isolated from SLE patients could be inhibited by gamma-IFN, but not by IL-2, IL-4 and IL-5. These data suggested that decreased functions of NK cells and related type 1 T helper cells be closely related to the immune dysregulation and autoantibodies production in SLE.

AB - Systemic lupus erythematosus (SLE) manifested with multiple autoantibodies production and glomerulonephritis, is the best example of systemic autoimmune diseases. To further elucidate the role of cytokines and the potential involvement of natural killer cells (NK cells) in the pathogenesis of lupus, phenotypic analysis of peripheral blood mononuclear cells (PBMC), NK cells cytotoxicity and cytokines production pattern of SLE patients and normal controls were examined. In addition, the effect of a variety of cytokines on anti-dsDNA antibodies production was also investigated. Our results showed that: (a) there was an increased percentage of memory T cells and decreased percentage of NK cells in SLE patients when compared to normal controls (p < 0.05); (b) a decreased production of cytokines like gamma-IFN in mitogen-stimulated PBMCs was also noted in SLE patients; (c) cytolytic activity of NK cells was markedly reduced in SLE patients (p < 0.05); (d) spontaneous secretion of IgG anti-dsDNA antibodies by B cells isolated from SLE patients could be inhibited by gamma-IFN, but not by IL-2, IL-4 and IL-5. These data suggested that decreased functions of NK cells and related type 1 T helper cells be closely related to the immune dysregulation and autoantibodies production in SLE.

UR - http://www.scopus.com/inward/record.url?scp=0029363959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029363959&partnerID=8YFLogxK

M3 - Article

C2 - 8607362

AN - SCOPUS:0029363959

VL - 36

SP - 357

EP - 363

JO - Pediatrics and Neonatology

JF - Pediatrics and Neonatology

SN - 1875-9572

IS - 5

ER -