Phosphorylation of CPAP by Aurora-A Maintains Spindle Pole Integrity during Mitosis

En Ju Chou, Liang Yi Hung, Chieh Ju C. Tang, Wen Bin Hsu, Hsin Yi Wu, Pao Chi Liao, Tang K. Tang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

CPAP is required for centriole elongation during S/G2 phase, but the role of CPAP in mitosis is incompletely understood. Here, we show that CPAP maintains spindle pole integrity through its phosphorylation by Aurora-A during mitosis. Depletion of CPAP induced a prolonged delay in mitosis, pericentriolar material (PCM) dispersion, and multiple mitotic abnormalities. Further studies demonstrated that CPAP directly interacts with and is phosphorylated by Aurora-A at serine 467 during mitosis. Interestingly, the dispersal of the PCM was effectively rescued by ectopic expression of wild-type CPAP or a phospho-mimic CPAP-S467D mutant, but not a non-phosphorylated CPAP-S467A mutant. Finally, we found that CPAP-S467D has a low affinity for microtubule binding but a high affinity for PCM proteins. Together, our results support a model wherein CPAP is required for proper mitotic progression, and phosphorylation of CPAP by Aurora-A is essential for maintaining spindle pole integrity. Chou et al. show that CPAP is essential for proper mitotic progression and maintenance of spindle pole integrity. CPAP is phosphorylated by Aurora-A during mitosis and phosphorylated CPAP coheres PCM proteins and maintains centrosome integrity.

Original languageEnglish
Pages (from-to)2975-2987
Number of pages13
JournalCell Reports
Volume14
Issue number12
DOIs
Publication statusPublished - 2016 Mar 29

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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