Photo fermentative hydrogen production using dominant components (acetate, lactate, and butyrate) in dark fermentation effluents

Yung Chung Lo, Chun Yen Chen, Chi Mei Lee, Jo Shu Chang

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Engineering strategies were applied to promote the phototrophic H 2 production of an indigenous purple nonsulfur bacterium Rhodopseudomonas palustris WP3-5 using major components (i.e., acetate, butyrate, and lactate) of dark fermentation effluents as carbon sources. First, performance of cell growth and photo-H2 production on each carbon source was examined individually. It appeared that acetate was the most effective carbon source for photo-H2 production, giving an overall H2 production rate and H2 yield of 12.68 ml/h/l and 67.1%, respectively. Next, the effect of substrate concentration of each carbon source on photo-hydrogen production was investigated. Kinetic models were developed to describe the correlation between maximum specific growth rate/specific H 2 production rate and the substrate concentration. The results show that using acetate and lactate as the carbon source, the kinetics for the cell growth and photo-hydrogen production can be described by Monod-type and Michaelis-Menten models, respectively, whereas substrate inhibition occurred when using butyrate as the carbon source. The continuous cultures were also conducted at a hydraulic retention time of 96 h using synthetic dark fermentation soluble metabolites (with a 5 and 10 fold dilution) as the influent. The phototrophic H2 production efficiency was stably maintained for over 30 days with an overall H2 yield 10.30 and 11.97 mol H2/mol sucrose, when using 5-fold and 10-fold diluted dark fermentation effluent, respectively, as the substrate for dark fermentation. This demonstrates the feasibility of using the sequential dark and photo fermentation for high-yield biohydrogen production.

Original languageEnglish
Pages (from-to)14059-14068
Number of pages10
JournalInternational Journal of Hydrogen Energy
Volume36
Issue number21
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

lactates
fermentation
effluents
hydrogen production
Hydrogen production
Fermentation
Effluents
acetates
carbon
Carbon
photoproduction
Cell growth
Substrates
metabolites
sucrose
kinetics
Kinetics
hydraulics
Sugar (sucrose)
bacteria

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

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title = "Photo fermentative hydrogen production using dominant components (acetate, lactate, and butyrate) in dark fermentation effluents",
abstract = "Engineering strategies were applied to promote the phototrophic H 2 production of an indigenous purple nonsulfur bacterium Rhodopseudomonas palustris WP3-5 using major components (i.e., acetate, butyrate, and lactate) of dark fermentation effluents as carbon sources. First, performance of cell growth and photo-H2 production on each carbon source was examined individually. It appeared that acetate was the most effective carbon source for photo-H2 production, giving an overall H2 production rate and H2 yield of 12.68 ml/h/l and 67.1{\%}, respectively. Next, the effect of substrate concentration of each carbon source on photo-hydrogen production was investigated. Kinetic models were developed to describe the correlation between maximum specific growth rate/specific H 2 production rate and the substrate concentration. The results show that using acetate and lactate as the carbon source, the kinetics for the cell growth and photo-hydrogen production can be described by Monod-type and Michaelis-Menten models, respectively, whereas substrate inhibition occurred when using butyrate as the carbon source. The continuous cultures were also conducted at a hydraulic retention time of 96 h using synthetic dark fermentation soluble metabolites (with a 5 and 10 fold dilution) as the influent. The phototrophic H2 production efficiency was stably maintained for over 30 days with an overall H2 yield 10.30 and 11.97 mol H2/mol sucrose, when using 5-fold and 10-fold diluted dark fermentation effluent, respectively, as the substrate for dark fermentation. This demonstrates the feasibility of using the sequential dark and photo fermentation for high-yield biohydrogen production.",
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Photo fermentative hydrogen production using dominant components (acetate, lactate, and butyrate) in dark fermentation effluents. / Lo, Yung Chung; Chen, Chun Yen; Lee, Chi Mei; Chang, Jo Shu.

In: International Journal of Hydrogen Energy, Vol. 36, No. 21, 01.10.2011, p. 14059-14068.

Research output: Contribution to journalArticle

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