Photoacoustic imaging of multiple targets using gold nanorods

Pai Chi Li; Chen Wei Wei; Chao Kang Liao; Cheng Dah Chen; Kuei Chen Pao; Churng Ren Chris Wang; Ya Na Wu; Dar Bin Shieh

doi:10.1109/TUFFC.2007.435

Photoacoustic imaging of multiple targets using gold nanorods

Pai Chi Li, Chen Wei Wei, Chao Kang Liao, Cheng Dah Chen, Kuei Chen Pao, Churng Ren Chris Wang, Ya Na Wu, Dar Bin Shieh

School of Dentistry

Research output: Contribution to journal › Article › peer-review

114 Citations (Scopus)

Abstract

Photoacoustlc (PA) Imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular Imaging, only single targeting has been demonstrated. In this study, PA Imaging of multiple targets using gold nanorods is demonstrated experimentally using HER2 and CXCR4 as target molecules. The two corresponding monoclonal antibodies were conjugated to two types of gold nanorod with different aspect ratios. Gold nanorods with mean aspect ratios of 5.9 and 3.7 exhibited peak optical absorptions at 1000 and 785 nm, respectively. Appropriate selection of laser irradiation wavelength enhances PA signals by 7-12 dB and allows signals from gold nanorods corresponding to specific bindings to be distinguished. This approach potentially allows the expression levels of different oncogenes of cancer cells to be revealed simultaneously.

Original language	English
Pages (from-to)	1642-1647
Number of pages	6
Journal	IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
Volume	54
Issue number	8
DOIs	https://doi.org/10.1109/TUFFC.2007.435
Publication status	Published - 2007 Aug

All Science Journal Classification (ASJC) codes

Instrumentation
Acoustics and Ultrasonics
Electrical and Electronic Engineering

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10.1109/TUFFC.2007.435

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@article{2ee84931a7f24f3c81206a8fbe9531a5,

title = "Photoacoustic imaging of multiple targets using gold nanorods",

abstract = "Photoacoustlc (PA) Imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular Imaging, only single targeting has been demonstrated. In this study, PA Imaging of multiple targets using gold nanorods is demonstrated experimentally using HER2 and CXCR4 as target molecules. The two corresponding monoclonal antibodies were conjugated to two types of gold nanorod with different aspect ratios. Gold nanorods with mean aspect ratios of 5.9 and 3.7 exhibited peak optical absorptions at 1000 and 785 nm, respectively. Appropriate selection of laser irradiation wavelength enhances PA signals by 7-12 dB and allows signals from gold nanorods corresponding to specific bindings to be distinguished. This approach potentially allows the expression levels of different oncogenes of cancer cells to be revealed simultaneously.",

author = "Li, {Pai Chi} and Wei, {Chen Wei} and Liao, {Chao Kang} and Chen, {Cheng Dah} and Pao, {Kuei Chen} and Wang, {Churng Ren Chris} and Wu, {Ya Na} and Shieh, {Dar Bin}",

note = "Funding Information: Manuscript received October 26, 2006; accepted March 23, 2007. Financial supports from National Science Council (grant# NSC 95-2218-E-002-036), National Health Research Institutes, NTU Center for Genomic Medicine, and NTU Nano Center for Science and Technology are gratefully acknowledged.",

year = "2007",

month = aug,

doi = "10.1109/TUFFC.2007.435",

language = "English",

volume = "54",

pages = "1642--1647",

journal = "IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control",

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publisher = "Institute of Electrical and Electronics Engineers Inc.",

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T1 - Photoacoustic imaging of multiple targets using gold nanorods

AU - Li, Pai Chi

AU - Wei, Chen Wei

AU - Liao, Chao Kang

AU - Chen, Cheng Dah

AU - Pao, Kuei Chen

AU - Wang, Churng Ren Chris

AU - Wu, Ya Na

AU - Shieh, Dar Bin

N1 - Funding Information: Manuscript received October 26, 2006; accepted March 23, 2007. Financial supports from National Science Council (grant# NSC 95-2218-E-002-036), National Health Research Institutes, NTU Center for Genomic Medicine, and NTU Nano Center for Science and Technology are gratefully acknowledged.

PY - 2007/8

Y1 - 2007/8

N2 - Photoacoustlc (PA) Imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular Imaging, only single targeting has been demonstrated. In this study, PA Imaging of multiple targets using gold nanorods is demonstrated experimentally using HER2 and CXCR4 as target molecules. The two corresponding monoclonal antibodies were conjugated to two types of gold nanorod with different aspect ratios. Gold nanorods with mean aspect ratios of 5.9 and 3.7 exhibited peak optical absorptions at 1000 and 785 nm, respectively. Appropriate selection of laser irradiation wavelength enhances PA signals by 7-12 dB and allows signals from gold nanorods corresponding to specific bindings to be distinguished. This approach potentially allows the expression levels of different oncogenes of cancer cells to be revealed simultaneously.

AB - Photoacoustlc (PA) Imaging has been used mainly for anatomical and functional imaging. Although functionalized nanoparticles also have been developed for PA molecular Imaging, only single targeting has been demonstrated. In this study, PA Imaging of multiple targets using gold nanorods is demonstrated experimentally using HER2 and CXCR4 as target molecules. The two corresponding monoclonal antibodies were conjugated to two types of gold nanorod with different aspect ratios. Gold nanorods with mean aspect ratios of 5.9 and 3.7 exhibited peak optical absorptions at 1000 and 785 nm, respectively. Appropriate selection of laser irradiation wavelength enhances PA signals by 7-12 dB and allows signals from gold nanorods corresponding to specific bindings to be distinguished. This approach potentially allows the expression levels of different oncogenes of cancer cells to be revealed simultaneously.

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JO - IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control

JF - IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control

IS - 8

ER -

Photoacoustic imaging of multiple targets using gold nanorods

Abstract

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