Photoautotrophic tobacco cells adapted to grow at high salinity

Ching-Chun Chang, R. D. Locy, R. Smeda, S. V. Sahi, N. K. Singh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed reduced growth on a fresh weight basis compared to P0 cells. Compared to P0 cells, intracellular sucrose levels were significantly higher in P20 cells while starch levels in P0 cells were significantly higher than in P20 cells. Levels of intracellular and extracellular reducing sugars, and chlorophyll accumulated to the same degree in P20 and P0 cells, but accumulation was delayed by approximately 13 days in P20 cells. O2 evolution and 14[CO2] fixation was more resistant to inhibition by NaCl in P20 cells than in P0 cells. However, significant changes in the abundance of thylakoid membrane proteins could not be demonstrated between P20 and P0 cells although higher levels of Rubisco on a per milligram chlorophyll basis were observed in P0 compared to P20 chloroplasts.

Original languageEnglish
Pages (from-to)495-502
Number of pages8
JournalPlant Cell Reports
Volume16
Issue number7
DOIs
Publication statusPublished - 1997 Jan 1

Fingerprint

tobacco
salinity
cells
chloroplasts
salts
chlorophyll
ribulose-bisphosphate carboxylase
reducing sugars
thylakoids
membrane proteins
Nicotiana tabacum
cell culture
starch
sucrose

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

Chang, Ching-Chun ; Locy, R. D. ; Smeda, R. ; Sahi, S. V. ; Singh, N. K. / Photoautotrophic tobacco cells adapted to grow at high salinity. In: Plant Cell Reports. 1997 ; Vol. 16, No. 7. pp. 495-502.
@article{05bee7980901454e8ec4c5c50be7a891,
title = "Photoautotrophic tobacco cells adapted to grow at high salinity",
abstract = "Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed reduced growth on a fresh weight basis compared to P0 cells. Compared to P0 cells, intracellular sucrose levels were significantly higher in P20 cells while starch levels in P0 cells were significantly higher than in P20 cells. Levels of intracellular and extracellular reducing sugars, and chlorophyll accumulated to the same degree in P20 and P0 cells, but accumulation was delayed by approximately 13 days in P20 cells. O2 evolution and 14[CO2] fixation was more resistant to inhibition by NaCl in P20 cells than in P0 cells. However, significant changes in the abundance of thylakoid membrane proteins could not be demonstrated between P20 and P0 cells although higher levels of Rubisco on a per milligram chlorophyll basis were observed in P0 compared to P20 chloroplasts.",
author = "Ching-Chun Chang and Locy, {R. D.} and R. Smeda and Sahi, {S. V.} and Singh, {N. K.}",
year = "1997",
month = "1",
day = "1",
doi = "10.1007/BF01092773",
language = "English",
volume = "16",
pages = "495--502",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer Verlag",
number = "7",

}

Chang, C-C, Locy, RD, Smeda, R, Sahi, SV & Singh, NK 1997, 'Photoautotrophic tobacco cells adapted to grow at high salinity', Plant Cell Reports, vol. 16, no. 7, pp. 495-502. https://doi.org/10.1007/BF01092773

Photoautotrophic tobacco cells adapted to grow at high salinity. / Chang, Ching-Chun; Locy, R. D.; Smeda, R.; Sahi, S. V.; Singh, N. K.

In: Plant Cell Reports, Vol. 16, No. 7, 01.01.1997, p. 495-502.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Photoautotrophic tobacco cells adapted to grow at high salinity

AU - Chang, Ching-Chun

AU - Locy, R. D.

AU - Smeda, R.

AU - Sahi, S. V.

AU - Singh, N. K.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed reduced growth on a fresh weight basis compared to P0 cells. Compared to P0 cells, intracellular sucrose levels were significantly higher in P20 cells while starch levels in P0 cells were significantly higher than in P20 cells. Levels of intracellular and extracellular reducing sugars, and chlorophyll accumulated to the same degree in P20 and P0 cells, but accumulation was delayed by approximately 13 days in P20 cells. O2 evolution and 14[CO2] fixation was more resistant to inhibition by NaCl in P20 cells than in P0 cells. However, significant changes in the abundance of thylakoid membrane proteins could not be demonstrated between P20 and P0 cells although higher levels of Rubisco on a per milligram chlorophyll basis were observed in P0 compared to P20 chloroplasts.

AB - Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed reduced growth on a fresh weight basis compared to P0 cells. Compared to P0 cells, intracellular sucrose levels were significantly higher in P20 cells while starch levels in P0 cells were significantly higher than in P20 cells. Levels of intracellular and extracellular reducing sugars, and chlorophyll accumulated to the same degree in P20 and P0 cells, but accumulation was delayed by approximately 13 days in P20 cells. O2 evolution and 14[CO2] fixation was more resistant to inhibition by NaCl in P20 cells than in P0 cells. However, significant changes in the abundance of thylakoid membrane proteins could not be demonstrated between P20 and P0 cells although higher levels of Rubisco on a per milligram chlorophyll basis were observed in P0 compared to P20 chloroplasts.

UR - http://www.scopus.com/inward/record.url?scp=0030787893&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030787893&partnerID=8YFLogxK

U2 - 10.1007/BF01092773

DO - 10.1007/BF01092773

M3 - Article

AN - SCOPUS:0030787893

VL - 16

SP - 495

EP - 502

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 7

ER -