Abstract
Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed reduced growth on a fresh weight basis compared to P0 cells. Compared to P0 cells, intracellular sucrose levels were significantly higher in P20 cells while starch levels in P0 cells were significantly higher than in P20 cells. Levels of intracellular and extracellular reducing sugars, and chlorophyll accumulated to the same degree in P20 and P0 cells, but accumulation was delayed by approximately 13 days in P20 cells. O2 evolution and 14[CO2] fixation was more resistant to inhibition by NaCl in P20 cells than in P0 cells. However, significant changes in the abundance of thylakoid membrane proteins could not be demonstrated between P20 and P0 cells although higher levels of Rubisco on a per milligram chlorophyll basis were observed in P0 compared to P20 chloroplasts.
| Original language | English |
|---|---|
| Pages (from-to) | 495-502 |
| Number of pages | 8 |
| Journal | Plant Cell Reports |
| Volume | 16 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 1997 Jan 1 |
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All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science
- Plant Science
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Photoautotrophic tobacco cells adapted to grow at high salinity. / Chang, Ching-Chun; Locy, R. D.; Smeda, R.; Sahi, S. V.; Singh, N. K.
In: Plant Cell Reports, Vol. 16, No. 7, 01.01.1997, p. 495-502.Research output: Contribution to journal › Article
TY - JOUR
T1 - Photoautotrophic tobacco cells adapted to grow at high salinity
AU - Chang, Ching-Chun
AU - Locy, R. D.
AU - Smeda, R.
AU - Sahi, S. V.
AU - Singh, N. K.
PY - 1997/1/1
Y1 - 1997/1/1
N2 - Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed reduced growth on a fresh weight basis compared to P0 cells. Compared to P0 cells, intracellular sucrose levels were significantly higher in P20 cells while starch levels in P0 cells were significantly higher than in P20 cells. Levels of intracellular and extracellular reducing sugars, and chlorophyll accumulated to the same degree in P20 and P0 cells, but accumulation was delayed by approximately 13 days in P20 cells. O2 evolution and 14[CO2] fixation was more resistant to inhibition by NaCl in P20 cells than in P0 cells. However, significant changes in the abundance of thylakoid membrane proteins could not be demonstrated between P20 and P0 cells although higher levels of Rubisco on a per milligram chlorophyll basis were observed in P0 compared to P20 chloroplasts.
AB - Photoautotrophic tobacco (Nicotiana tabacum var. Wisconsin 38) cell cultures were gradually adapted to grow in media containing the normally inhibitory concentration of 20 g l-1 NaCl. Both salt-adapted cultures maintained in 20 g l-1 NaCl (P20) and salt-unadapted (P0) cultures demonstrated similar chloroplast morphology and similar growth characteristics on a dry weight basis, but P20 cells showed reduced growth on a fresh weight basis compared to P0 cells. Compared to P0 cells, intracellular sucrose levels were significantly higher in P20 cells while starch levels in P0 cells were significantly higher than in P20 cells. Levels of intracellular and extracellular reducing sugars, and chlorophyll accumulated to the same degree in P20 and P0 cells, but accumulation was delayed by approximately 13 days in P20 cells. O2 evolution and 14[CO2] fixation was more resistant to inhibition by NaCl in P20 cells than in P0 cells. However, significant changes in the abundance of thylakoid membrane proteins could not be demonstrated between P20 and P0 cells although higher levels of Rubisco on a per milligram chlorophyll basis were observed in P0 compared to P20 chloroplasts.
UR - http://www.scopus.com/inward/record.url?scp=0030787893&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030787893&partnerID=8YFLogxK
U2 - 10.1007/BF01092773
DO - 10.1007/BF01092773
M3 - Article
AN - SCOPUS:0030787893
VL - 16
SP - 495
EP - 502
JO - Plant Cell Reports
JF - Plant Cell Reports
SN - 0721-7714
IS - 7
ER -