Phototrophic cultivation of a thermo-tolerant Desmodesmus sp. for lutein production: Effects of nitrate concentration, light intensity and fed-batch operation

Youping Xie, Shih Hsin Ho, Ching Nen Nathan Chen, Chun Yen Chen, I. Son Ng, Ke Ju Jing, Jo Shu Chang, Yinghua Lu

Research output: Contribution to journalArticlepeer-review

82 Citations (Scopus)

Abstract

Four indigenous thermo-tolerant Desmodesmus sp. strains were examined for their ability to produce lutein. Among them, Desmodesmus sp. F51 was the best strain for this purpose. The medium composition, nitrate concentration and light intensity were manipulated to improve the phototrophic growth and lutein production of Desmodesmus sp. F51. It was found that a nitrogen-sufficient condition was required for lutein accumulation, while a high light intensity enhanced cell growth but caused a decrease in the lutein content. The best cell growth and lutein production occurred when the light intensity and initial nitrate concentration were 600μmol/m2/s and 8.8mM, respectively. The fed-batch cultivation strategy was shown to further improve lutein production. The highest lutein productivity (3.56±0.10mg/L/d) and content (5.05±0.20mg/g) were obtained when pulse-feeding of 2.2mM nitrate was employed. This study demonstrated the potential of using Desmodesmus sp. F51 as a lutein producer in practical applications.

Original languageEnglish
Pages (from-to)435-444
Number of pages10
JournalBioresource technology
Volume144
DOIs
Publication statusPublished - 2013 Sep

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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