TY - JOUR
T1 - Phthalate exposure alters gut microbiota composition and IgM vaccine response in human newborns
AU - Yang, Yung Ning
AU - Yang, Yu Chen S.H.
AU - Lin, I. Hsuan
AU - Chen, Ying Yu
AU - Lin, Hung Yun
AU - Wu, Chien Yi
AU - Su, Yu Tsun
AU - Yang, Yao Jong
AU - Yang, San Nan
AU - Suen, Jau Ling
N1 - Funding Information:
This work was supported by Ministry of Science and Technology grant (MOST 106-2320-B-037-018, MOST 106-2314-B-037-072), grants of E-Da hospital (EDAHP105023, EDAHP106013, EDAHI107005 and NCKUEDA10508), and the “KMU Research Center for Environmental Medicine” or “TMU Research Center of Cancer Translational Medicine” (DP2-107-20000) from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan. We thank the Center for Research Resources and Development of KMU for providing LSRII, TissueFAXS, Waters UPLC, and LC-MS/MS systems. We thank the Cancer Translational Core Facility of Taipei Medical University for 16S rRNA gene sequencing and analysis. We thank Chyi-Her Lin, MD, PhD for critically revising the manuscript.
Funding Information:
This work was supported by Ministry of Science and Technology grant ( MOST 106-2320-B-037-018 , MOST 106-2314-B-037-072 ), grants of E-Da hospital ( EDAHP105023 , EDAHP106013 , EDAHI107005 and NCKUEDA10508 ), and the “KMU Research Center for Environmental Medicine” or “TMU Research Center of Cancer Translational Medicine” ( DP2-107-20000 ) from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan . We thank the Center for Research Resources and Development of KMU for providing LSRII, TissueFAXS, Waters UPLC, and LC-MS/MS systems. We thank the Cancer Translational Core Facility of Taipei Medical University for 16S rRNA gene sequencing and analysis. We thank Chyi-Her Lin, MD, PhD for critically revising the manuscript.
Publisher Copyright:
© 2019 The Authors
PY - 2019/10
Y1 - 2019/10
N2 - Postnatal exposure to di-(2-ethylhexyl) phthalate (DEHP), a common plasticizer, is associated with allergy development in childhood, suggesting that DEHP exposure may dysregulate immune response in infants. We investigated whether DEHP exposure in newborns through medical treatment affected the gut microbiota pattern and vaccine response, which are both related to immune development. In this prospective cohort study from May 1, 2016 through July 31, 2017, newborns with respiratory distress who were given intravenous infusions (IVs) were enrolled as the DEHP group, and newborns who did not receive IVs were enrolled as the control group. We excluded patients with perinatal maternal probiotics, vaginal delivery, antibiotic treatment, and exclusive human milk or formula feeding. Of 118 infants, urinary phthalate metabolite analysis revealed that the calculated DEHP concentrations of the newborns treated with IVs (n = 15) were higher than those in the control group (n = 10) (p = 0.0001). DEHP exposure altered bacterial communities both in composition and diversity, particularly decreases in Rothia sp. and Bifidobacterium longum in the DEHP group. Furthermore, DEHP exposure significantly enhanced anti-HBsAg-IgM responses in the DEHP group (p = 0.013). Early-life DEHP exposure alter gut microbiota of newborns and may change their immune responses in later life.
AB - Postnatal exposure to di-(2-ethylhexyl) phthalate (DEHP), a common plasticizer, is associated with allergy development in childhood, suggesting that DEHP exposure may dysregulate immune response in infants. We investigated whether DEHP exposure in newborns through medical treatment affected the gut microbiota pattern and vaccine response, which are both related to immune development. In this prospective cohort study from May 1, 2016 through July 31, 2017, newborns with respiratory distress who were given intravenous infusions (IVs) were enrolled as the DEHP group, and newborns who did not receive IVs were enrolled as the control group. We excluded patients with perinatal maternal probiotics, vaginal delivery, antibiotic treatment, and exclusive human milk or formula feeding. Of 118 infants, urinary phthalate metabolite analysis revealed that the calculated DEHP concentrations of the newborns treated with IVs (n = 15) were higher than those in the control group (n = 10) (p = 0.0001). DEHP exposure altered bacterial communities both in composition and diversity, particularly decreases in Rothia sp. and Bifidobacterium longum in the DEHP group. Furthermore, DEHP exposure significantly enhanced anti-HBsAg-IgM responses in the DEHP group (p = 0.013). Early-life DEHP exposure alter gut microbiota of newborns and may change their immune responses in later life.
UR - http://www.scopus.com/inward/record.url?scp=85069839464&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85069839464&partnerID=8YFLogxK
U2 - 10.1016/j.fct.2019.110700
DO - 10.1016/j.fct.2019.110700
M3 - Article
C2 - 31356917
AN - SCOPUS:85069839464
SN - 0278-6915
VL - 132
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
M1 - 110700
ER -