Phyto-fabrication of silver nanoparticles by Acacia nilotica leaves: Investigating their antineoplastic, free radical scavenging potential and application in H 2 O 2 sensing

Rijuta Ganesh Saratale, Ganesh Dattatraya Saratale, Si Kyung Cho, Gajanan Ghodake, Avinash Kadam, Sunil Kumar, Sikandar I. Mulla, Dong Su Kim, Byong Hun Jeon, Jo Shu Chang, Han Seung Shin

Research output: Contribution to journalArticle

Abstract

In this study, a green approach by utilizing Acacia nilotica leaves (ANL) extract to produce silver nanoparticles (AgNPs) has been made. The biosynthesized AgNPs displayed surface plasmon resonance (SPR) centered at 430 nm. Fourier transform infrared spectroscopy (FTIR) and energy-dispersive spectra (EDS) results showed the contribution of phyto-constituents of ANL in the reduction, capping and stabilizing matrix for AgNPs. The AgNPs were characterized by X-ray diffraction (XRD), and high resolution transmission electron microscopy (HR-TEM) revealed the synthesized ANL-AgNPs were crystalline and spherical forms having about ∼20 nm average particle size. ANL-AgNPs exhibited significant antineoplastic efficacy towards SKOV3 ovarian cancer cells (LD 50 ; 66.5 µg/mL) thus could be useful to treat the cancer and to trounce limitations of conventional chemotherapy treatment. The synthesized AgNPs unveiled substantial antioxidant activity represented by common enzyme markers 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (IC 50 ; 48.4 µg/mL) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) (IC 50 ; 55.2 µg/mL) activity. The ANL-AgNPs displayed substantial anti-bacterial activity against human pathogenic bacterial strains and also studied their minimum inhibitory and minimum bactericidal concentration (MIC and MBC) for individual strain. Additionally, the synthesized ANL-AgNPs exhibited colorimetric detection of hydrogen peroxide (H 2 O 2 ) with better selectivity which showed its viability in determining reactive oxygen species in biological and environmental samples. Overall, the ANL-AgNPs could be used in multitasking biomedical applications, including anticancer, antioxidant, and antibacterial potential and also in sensing application.

Original languageEnglish
Pages (from-to)239-249
Number of pages11
JournalJournal of the Taiwan Institute of Chemical Engineers
Volume99
DOIs
Publication statusPublished - 2019 Jun

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Scavenging
Antioxidants
Free radicals
Silver
Antineoplastic Agents
Free Radicals
Nanoparticles
Fabrication
Multitasking
Chemotherapy
Sulfonic Acids
Surface plasmon resonance
High resolution transmission electron microscopy
Hydrogen peroxide
Hydrogen Peroxide
Fourier transform infrared spectroscopy
Reactive Oxygen Species
Enzymes
Particle size
Cells

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Saratale, Rijuta Ganesh ; Saratale, Ganesh Dattatraya ; Cho, Si Kyung ; Ghodake, Gajanan ; Kadam, Avinash ; Kumar, Sunil ; Mulla, Sikandar I. ; Kim, Dong Su ; Jeon, Byong Hun ; Chang, Jo Shu ; Shin, Han Seung. / Phyto-fabrication of silver nanoparticles by Acacia nilotica leaves : Investigating their antineoplastic, free radical scavenging potential and application in H 2 O 2 sensing. In: Journal of the Taiwan Institute of Chemical Engineers. 2019 ; Vol. 99. pp. 239-249.
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abstract = "In this study, a green approach by utilizing Acacia nilotica leaves (ANL) extract to produce silver nanoparticles (AgNPs) has been made. The biosynthesized AgNPs displayed surface plasmon resonance (SPR) centered at 430 nm. Fourier transform infrared spectroscopy (FTIR) and energy-dispersive spectra (EDS) results showed the contribution of phyto-constituents of ANL in the reduction, capping and stabilizing matrix for AgNPs. The AgNPs were characterized by X-ray diffraction (XRD), and high resolution transmission electron microscopy (HR-TEM) revealed the synthesized ANL-AgNPs were crystalline and spherical forms having about ∼20 nm average particle size. ANL-AgNPs exhibited significant antineoplastic efficacy towards SKOV3 ovarian cancer cells (LD 50 ; 66.5 µg/mL) thus could be useful to treat the cancer and to trounce limitations of conventional chemotherapy treatment. The synthesized AgNPs unveiled substantial antioxidant activity represented by common enzyme markers 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (IC 50 ; 48.4 µg/mL) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) (IC 50 ; 55.2 µg/mL) activity. The ANL-AgNPs displayed substantial anti-bacterial activity against human pathogenic bacterial strains and also studied their minimum inhibitory and minimum bactericidal concentration (MIC and MBC) for individual strain. Additionally, the synthesized ANL-AgNPs exhibited colorimetric detection of hydrogen peroxide (H 2 O 2 ) with better selectivity which showed its viability in determining reactive oxygen species in biological and environmental samples. Overall, the ANL-AgNPs could be used in multitasking biomedical applications, including anticancer, antioxidant, and antibacterial potential and also in sensing application.",
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Phyto-fabrication of silver nanoparticles by Acacia nilotica leaves : Investigating their antineoplastic, free radical scavenging potential and application in H 2 O 2 sensing. / Saratale, Rijuta Ganesh; Saratale, Ganesh Dattatraya; Cho, Si Kyung; Ghodake, Gajanan; Kadam, Avinash; Kumar, Sunil; Mulla, Sikandar I.; Kim, Dong Su; Jeon, Byong Hun; Chang, Jo Shu; Shin, Han Seung.

In: Journal of the Taiwan Institute of Chemical Engineers, Vol. 99, 06.2019, p. 239-249.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Phyto-fabrication of silver nanoparticles by Acacia nilotica leaves

T2 - Investigating their antineoplastic, free radical scavenging potential and application in H 2 O 2 sensing

AU - Saratale, Rijuta Ganesh

AU - Saratale, Ganesh Dattatraya

AU - Cho, Si Kyung

AU - Ghodake, Gajanan

AU - Kadam, Avinash

AU - Kumar, Sunil

AU - Mulla, Sikandar I.

AU - Kim, Dong Su

AU - Jeon, Byong Hun

AU - Chang, Jo Shu

AU - Shin, Han Seung

PY - 2019/6

Y1 - 2019/6

N2 - In this study, a green approach by utilizing Acacia nilotica leaves (ANL) extract to produce silver nanoparticles (AgNPs) has been made. The biosynthesized AgNPs displayed surface plasmon resonance (SPR) centered at 430 nm. Fourier transform infrared spectroscopy (FTIR) and energy-dispersive spectra (EDS) results showed the contribution of phyto-constituents of ANL in the reduction, capping and stabilizing matrix for AgNPs. The AgNPs were characterized by X-ray diffraction (XRD), and high resolution transmission electron microscopy (HR-TEM) revealed the synthesized ANL-AgNPs were crystalline and spherical forms having about ∼20 nm average particle size. ANL-AgNPs exhibited significant antineoplastic efficacy towards SKOV3 ovarian cancer cells (LD 50 ; 66.5 µg/mL) thus could be useful to treat the cancer and to trounce limitations of conventional chemotherapy treatment. The synthesized AgNPs unveiled substantial antioxidant activity represented by common enzyme markers 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (IC 50 ; 48.4 µg/mL) and 2,2-Diphenyl-1-picrylhydrazyl (DPPH) (IC 50 ; 55.2 µg/mL) activity. The ANL-AgNPs displayed substantial anti-bacterial activity against human pathogenic bacterial strains and also studied their minimum inhibitory and minimum bactericidal concentration (MIC and MBC) for individual strain. Additionally, the synthesized ANL-AgNPs exhibited colorimetric detection of hydrogen peroxide (H 2 O 2 ) with better selectivity which showed its viability in determining reactive oxygen species in biological and environmental samples. Overall, the ANL-AgNPs could be used in multitasking biomedical applications, including anticancer, antioxidant, and antibacterial potential and also in sensing application.

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