Pilot-scale hydrogen fermentation system start-up performance

Chiu Yue Lin, Shu Yii Wu, Ping Jei Lin, Jo Shu Chang, Chun Hsiung Hung, Kuo Shing Lee, Feng Yuan Chang, Chen Yeon Chu, Chin Hung Cheng, Chyi How Lay, Alex C. Chang

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


A high-rate hydrogen production process able to produce H2 at a maximum rate of 15 L/L/h was successfully developed by the Feng Chia University (FCU) biohydrogen research team. This highly efficient hydrogen fermentation system includes a 400 L pilot-scale system constructed for determining scale-up operation parameters for commercializing the bioH2 production technology. The pilot-scale system is composed of a feedstock tank, mixing system, fermentor, gas/liquid separator and automatic control system. The fermentor is fed with sucrose (20 g COD/L) and operated at 35 °C. A batch strategy is used for system start-up. The fermentor was first operated in a batch mode for two days and then switched to a continuous-feeding mode (HRT 12 h) for one month. During the continuous operation, pH notably affected H 2 production efficiency and bacterial community. For the first 14-day operation, the H2 production rate increased from 0.017 to 0.256 L/L/h with a pH variation from 5.0 to 7.0. The DGGE results indicate the presence of two Clostridium species (namely, Clostridium butyricum and Clostridium pasteurianum) in the fermenter. Stable hydrogen production rate was obtained at pH 5.5-6.0 when C. pasteurianum became dominant in the mixed culture.

Original languageEnglish
Pages (from-to)13452-13457
Number of pages6
JournalInternational Journal of Hydrogen Energy
Issue number24
Publication statusPublished - 2010 Dec

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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