Pilot study on ex situ bioremediation of diesel-contaminated soil using bioaugmentation and biostimulation

Sheng Shung Cheng, Po Tsen Pan, Liang Ming Whang, Pao Wen Liu, I. Cheng Tseng, Jo Shu Chang, Tsung Chung Chang, Chiu Chung Young

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Soil at a site that recently was contaminated by diesel was investigated to apply different enhanced bioremediation processes at a pilot scale of 0.5m 3 biopiles. The indigenous microbial concentration was measured at the range of 10 5 CFU/g soil that meaned the biodegradation would be happened. After two months of soil stabilization, a certain amount of diesel was applied again to these four acclimated biopiles at the level of 4,500 to 5,000 mg TPH-d/kg dry soil. Same dosages of the enriched diesel-degrading bacteria, rhamnolipid, and BH nutrients were applied to BA1, BS, and NE biopiles. A second run of landfarming was evaluated with same performance indicators. Within the first 30 days, the plate counting of existed soil culture was measured at the range of 10 6 to 10 7 CFU/g soil. The ratios of TPH-d degrading colonies (HDB) to total plate count (HAB) were enhanced with 9% in Ct, 49% in NE, 45% in BA1, and 42% in BS, respectively. Therefore, the TPH-d removal efficiencies achieved at different levels in these four biopiles, Ct=15%, NE=74%, BA1=77%, BS=40%. Bioremediation performance was promoted by bioaugmentation, while the biosurfactant addition (BS) attainted fair performance. The indigenous biopile (Ct) was still inhibited by the high loading of diesel with 3,600 mg TPH-d/kg dry soil. First-order reaction rate constant K values (day -1) were evaluated as BAl(0.0592)>NE(0.0442)>BS(0. 0205)>Ct(0.0065). Molecular biomonitoring methods were developed to identify the diesel-degrading bacteria existing in all biopiles: DGGE electrophoresis showed the predominant group was Pseudomonas sp. presented in three biopiles of NE, BA1 and BS. This pilot study of three months of biopile farming approved that bioaugmentation and biostimulation could enhance the bioremediation of TPH-d-contaminated soil.

Original languageEnglish
Title of host publicationBattelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
Pages412-419
Number of pages8
Publication statusPublished - 2007 Dec 1
Event9th International In Situ and On-Site Bioremediation Symposium 2007 - Baltimore, MD, United States
Duration: 2007 May 72007 May 10

Publication series

NameBattelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007
Volume1

Other

Other9th International In Situ and On-Site Bioremediation Symposium 2007
CountryUnited States
CityBaltimore, MD
Period07-05-0707-05-10

Fingerprint

Bioremediation
bioremediation
diesel
Soils
soil
soil stabilization
Bacteria
bacterium
biomonitoring
reaction rate
contaminated soil
biodegradation
electrokinesis
Biodegradation
Electrophoresis
Nutrients
Reaction rates
Rate constants
Stabilization
nutrient

All Science Journal Classification (ASJC) codes

  • Ecological Modelling
  • Environmental Engineering
  • Bioengineering
  • Waste Management and Disposal

Cite this

Cheng, S. S., Pan, P. T., Whang, L. M., Liu, P. W., Tseng, I. C., Chang, J. S., ... Young, C. C. (2007). Pilot study on ex situ bioremediation of diesel-contaminated soil using bioaugmentation and biostimulation. In Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007 (pp. 412-419). (Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007; Vol. 1).
Cheng, Sheng Shung ; Pan, Po Tsen ; Whang, Liang Ming ; Liu, Pao Wen ; Tseng, I. Cheng ; Chang, Jo Shu ; Chang, Tsung Chung ; Young, Chiu Chung. / Pilot study on ex situ bioremediation of diesel-contaminated soil using bioaugmentation and biostimulation. Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007. 2007. pp. 412-419 (Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007).
@inproceedings{0832d6c9974b484aa2594c4f3a493057,
title = "Pilot study on ex situ bioremediation of diesel-contaminated soil using bioaugmentation and biostimulation",
abstract = "Soil at a site that recently was contaminated by diesel was investigated to apply different enhanced bioremediation processes at a pilot scale of 0.5m 3 biopiles. The indigenous microbial concentration was measured at the range of 10 5 CFU/g soil that meaned the biodegradation would be happened. After two months of soil stabilization, a certain amount of diesel was applied again to these four acclimated biopiles at the level of 4,500 to 5,000 mg TPH-d/kg dry soil. Same dosages of the enriched diesel-degrading bacteria, rhamnolipid, and BH nutrients were applied to BA1, BS, and NE biopiles. A second run of landfarming was evaluated with same performance indicators. Within the first 30 days, the plate counting of existed soil culture was measured at the range of 10 6 to 10 7 CFU/g soil. The ratios of TPH-d degrading colonies (HDB) to total plate count (HAB) were enhanced with 9{\%} in Ct, 49{\%} in NE, 45{\%} in BA1, and 42{\%} in BS, respectively. Therefore, the TPH-d removal efficiencies achieved at different levels in these four biopiles, Ct=15{\%}, NE=74{\%}, BA1=77{\%}, BS=40{\%}. Bioremediation performance was promoted by bioaugmentation, while the biosurfactant addition (BS) attainted fair performance. The indigenous biopile (Ct) was still inhibited by the high loading of diesel with 3,600 mg TPH-d/kg dry soil. First-order reaction rate constant K values (day -1) were evaluated as BAl(0.0592)>NE(0.0442)>BS(0. 0205)>Ct(0.0065). Molecular biomonitoring methods were developed to identify the diesel-degrading bacteria existing in all biopiles: DGGE electrophoresis showed the predominant group was Pseudomonas sp. presented in three biopiles of NE, BA1 and BS. This pilot study of three months of biopile farming approved that bioaugmentation and biostimulation could enhance the bioremediation of TPH-d-contaminated soil.",
author = "Cheng, {Sheng Shung} and Pan, {Po Tsen} and Whang, {Liang Ming} and Liu, {Pao Wen} and Tseng, {I. Cheng} and Chang, {Jo Shu} and Chang, {Tsung Chung} and Young, {Chiu Chung}",
year = "2007",
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isbn = "9781604239485",
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Cheng, SS, Pan, PT, Whang, LM, Liu, PW, Tseng, IC, Chang, JS, Chang, TC & Young, CC 2007, Pilot study on ex situ bioremediation of diesel-contaminated soil using bioaugmentation and biostimulation. in Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007. Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007, vol. 1, pp. 412-419, 9th International In Situ and On-Site Bioremediation Symposium 2007, Baltimore, MD, United States, 07-05-07.

Pilot study on ex situ bioremediation of diesel-contaminated soil using bioaugmentation and biostimulation. / Cheng, Sheng Shung; Pan, Po Tsen; Whang, Liang Ming; Liu, Pao Wen; Tseng, I. Cheng; Chang, Jo Shu; Chang, Tsung Chung; Young, Chiu Chung.

Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007. 2007. p. 412-419 (Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007; Vol. 1).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Pilot study on ex situ bioremediation of diesel-contaminated soil using bioaugmentation and biostimulation

AU - Cheng, Sheng Shung

AU - Pan, Po Tsen

AU - Whang, Liang Ming

AU - Liu, Pao Wen

AU - Tseng, I. Cheng

AU - Chang, Jo Shu

AU - Chang, Tsung Chung

AU - Young, Chiu Chung

PY - 2007/12/1

Y1 - 2007/12/1

N2 - Soil at a site that recently was contaminated by diesel was investigated to apply different enhanced bioremediation processes at a pilot scale of 0.5m 3 biopiles. The indigenous microbial concentration was measured at the range of 10 5 CFU/g soil that meaned the biodegradation would be happened. After two months of soil stabilization, a certain amount of diesel was applied again to these four acclimated biopiles at the level of 4,500 to 5,000 mg TPH-d/kg dry soil. Same dosages of the enriched diesel-degrading bacteria, rhamnolipid, and BH nutrients were applied to BA1, BS, and NE biopiles. A second run of landfarming was evaluated with same performance indicators. Within the first 30 days, the plate counting of existed soil culture was measured at the range of 10 6 to 10 7 CFU/g soil. The ratios of TPH-d degrading colonies (HDB) to total plate count (HAB) were enhanced with 9% in Ct, 49% in NE, 45% in BA1, and 42% in BS, respectively. Therefore, the TPH-d removal efficiencies achieved at different levels in these four biopiles, Ct=15%, NE=74%, BA1=77%, BS=40%. Bioremediation performance was promoted by bioaugmentation, while the biosurfactant addition (BS) attainted fair performance. The indigenous biopile (Ct) was still inhibited by the high loading of diesel with 3,600 mg TPH-d/kg dry soil. First-order reaction rate constant K values (day -1) were evaluated as BAl(0.0592)>NE(0.0442)>BS(0. 0205)>Ct(0.0065). Molecular biomonitoring methods were developed to identify the diesel-degrading bacteria existing in all biopiles: DGGE electrophoresis showed the predominant group was Pseudomonas sp. presented in three biopiles of NE, BA1 and BS. This pilot study of three months of biopile farming approved that bioaugmentation and biostimulation could enhance the bioremediation of TPH-d-contaminated soil.

AB - Soil at a site that recently was contaminated by diesel was investigated to apply different enhanced bioremediation processes at a pilot scale of 0.5m 3 biopiles. The indigenous microbial concentration was measured at the range of 10 5 CFU/g soil that meaned the biodegradation would be happened. After two months of soil stabilization, a certain amount of diesel was applied again to these four acclimated biopiles at the level of 4,500 to 5,000 mg TPH-d/kg dry soil. Same dosages of the enriched diesel-degrading bacteria, rhamnolipid, and BH nutrients were applied to BA1, BS, and NE biopiles. A second run of landfarming was evaluated with same performance indicators. Within the first 30 days, the plate counting of existed soil culture was measured at the range of 10 6 to 10 7 CFU/g soil. The ratios of TPH-d degrading colonies (HDB) to total plate count (HAB) were enhanced with 9% in Ct, 49% in NE, 45% in BA1, and 42% in BS, respectively. Therefore, the TPH-d removal efficiencies achieved at different levels in these four biopiles, Ct=15%, NE=74%, BA1=77%, BS=40%. Bioremediation performance was promoted by bioaugmentation, while the biosurfactant addition (BS) attainted fair performance. The indigenous biopile (Ct) was still inhibited by the high loading of diesel with 3,600 mg TPH-d/kg dry soil. First-order reaction rate constant K values (day -1) were evaluated as BAl(0.0592)>NE(0.0442)>BS(0. 0205)>Ct(0.0065). Molecular biomonitoring methods were developed to identify the diesel-degrading bacteria existing in all biopiles: DGGE electrophoresis showed the predominant group was Pseudomonas sp. presented in three biopiles of NE, BA1 and BS. This pilot study of three months of biopile farming approved that bioaugmentation and biostimulation could enhance the bioremediation of TPH-d-contaminated soil.

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Cheng SS, Pan PT, Whang LM, Liu PW, Tseng IC, Chang JS et al. Pilot study on ex situ bioremediation of diesel-contaminated soil using bioaugmentation and biostimulation. In Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007. 2007. p. 412-419. (Battelle Press - 9th International In Situ and On-Site Bioremediation Symposium 2007).