Planar patch-clamp chip integrated with microfluidic concentration generator for rapid solution exchange

Chang Yu Chen, Kuan Ting Liu, Ting-Yuan Tu, De Shien Jong, Andrew M. Wo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Planar patch-clamp chips have been developed in varies approaches to simplify ion-channel signal detection. Integration with microfluidics has mainly focused on delivery of prepared solutions to the chip. This work presents the integration of planar patch-clamp chip with microfluidic concentration generator for rapid solution exchange. Different concentrations of fluid could be rapidly delivered and exchanged by adjusting relative flow rate of each inlet, and fully mixed through a micromixer. Endogenous chloride channels in human embryonic kidney 293T cells were successfully examined in various osmolarities generated by the concentration generator. This practical approach demonstrates the potential of integrated microfluidic patch-clamp system for high throughput ion channel screening of drugs.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages506-508
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09-11-0109-11-05

Fingerprint

Clamping devices
Microfluidics
Ion exchange
Ion Channels
Chloride Channels
Signal detection
Ions
Screening
Flow rate
Throughput
Fluids
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Chen, C. Y., Liu, K. T., Tu, T-Y., Jong, D. S., & Wo, A. M. (2009). Planar patch-clamp chip integrated with microfluidic concentration generator for rapid solution exchange. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 506-508). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.
Chen, Chang Yu ; Liu, Kuan Ting ; Tu, Ting-Yuan ; Jong, De Shien ; Wo, Andrew M. / Planar patch-clamp chip integrated with microfluidic concentration generator for rapid solution exchange. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 506-508 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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Chen, CY, Liu, KT, Tu, T-Y, Jong, DS & Wo, AM 2009, Planar patch-clamp chip integrated with microfluidic concentration generator for rapid solution exchange. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 506-508, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 09-11-01.

Planar patch-clamp chip integrated with microfluidic concentration generator for rapid solution exchange. / Chen, Chang Yu; Liu, Kuan Ting; Tu, Ting-Yuan; Jong, De Shien; Wo, Andrew M.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 506-508 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Chen CY, Liu KT, Tu T-Y, Jong DS, Wo AM. Planar patch-clamp chip integrated with microfluidic concentration generator for rapid solution exchange. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 506-508. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).