Abstract
Planar patch-clamp chips have been developed in varies approaches to simplify ion-channel signal detection. Integration with microfluidics has mainly focused on delivery of prepared solutions to the chip. This work presents the integration of planar patch-clamp chip with microfluidic concentration generator for rapid solution exchange. Different concentrations of fluid could be rapidly delivered and exchanged by adjusting relative flow rate of each inlet, and fully mixed through a micromixer. Endogenous chloride channels in human embryonic kidney 293T cells were successfully examined in various osmolarities generated by the concentration generator. This practical approach demonstrates the potential of integrated microfluidic patch-clamp system for high throughput ion channel screening of drugs.
| Original language | English |
|---|---|
| Title of host publication | Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 506-508 |
| Number of pages | 3 |
| ISBN (Print) | 9780979806421 |
| Publication status | Published - 2009 Jan 1 |
| Event | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of Duration: 2009 Nov 1 → 2009 Nov 5 |
Publication series
| Name | Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
|---|
Other
| Other | 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 |
|---|---|
| Country | Korea, Republic of |
| City | Jeju |
| Period | 09-11-01 → 09-11-05 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Chemical Engineering (miscellaneous)
- Bioengineering
Cite this
}
Planar patch-clamp chip integrated with microfluidic concentration generator for rapid solution exchange. / Chen, Chang Yu; Liu, Kuan Ting; Tu, Ting-Yuan; Jong, De Shien; Wo, Andrew M.
Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 506-508 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Planar patch-clamp chip integrated with microfluidic concentration generator for rapid solution exchange
AU - Chen, Chang Yu
AU - Liu, Kuan Ting
AU - Tu, Ting-Yuan
AU - Jong, De Shien
AU - Wo, Andrew M.
PY - 2009/1/1
Y1 - 2009/1/1
N2 - Planar patch-clamp chips have been developed in varies approaches to simplify ion-channel signal detection. Integration with microfluidics has mainly focused on delivery of prepared solutions to the chip. This work presents the integration of planar patch-clamp chip with microfluidic concentration generator for rapid solution exchange. Different concentrations of fluid could be rapidly delivered and exchanged by adjusting relative flow rate of each inlet, and fully mixed through a micromixer. Endogenous chloride channels in human embryonic kidney 293T cells were successfully examined in various osmolarities generated by the concentration generator. This practical approach demonstrates the potential of integrated microfluidic patch-clamp system for high throughput ion channel screening of drugs.
AB - Planar patch-clamp chips have been developed in varies approaches to simplify ion-channel signal detection. Integration with microfluidics has mainly focused on delivery of prepared solutions to the chip. This work presents the integration of planar patch-clamp chip with microfluidic concentration generator for rapid solution exchange. Different concentrations of fluid could be rapidly delivered and exchanged by adjusting relative flow rate of each inlet, and fully mixed through a micromixer. Endogenous chloride channels in human embryonic kidney 293T cells were successfully examined in various osmolarities generated by the concentration generator. This practical approach demonstrates the potential of integrated microfluidic patch-clamp system for high throughput ion channel screening of drugs.
UR - http://www.scopus.com/inward/record.url?scp=84901799515&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84901799515&partnerID=8YFLogxK
M3 - Conference contribution
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 506
EP - 508
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
ER -