Plasmid-mediated quinolone resistance determinants in quinolone-resistant Escherichia coli isolated from patients with bacteremia in a university hospital in Taiwan, 2001-2015

Cheng Yen Kao, Hsiu Mei Wu, Wei-Hung Lin, Chin-Chung Tseng, Jing Jou Yan, Ming-Cheng Wang, Ching-Hao Teng, Jiunn Jong Wu

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Abstract

The aim of this study was to characterize fluoroquinolone (FQ)-resistant Escherichia coli isolates from bacteremia in Taiwan in 2001-2015. During the study period, 248 (21.2%) of 1171 isolates were identified as levofloxacin-resistant. The results of phylogenetic group analysis showed that 38.7% of the FQ-resistant isolates belonged to phylogenetic group B2, 23.4% to group B1, 22.6% to groupA, 14.9% to group D, and 0.4% belonged to group F. FQ-resistant isolates were highly susceptible to cefepime (91.5%), imipenem (96.0%), meropenem (98.8%), amikacin (98.0%), and fosfomycin (99.6%), as determined by the agar dilution method. β-lactamases, including bla TEM (66.1%), bla CMY-2 (16.5%), bla CTX-M (5.2%), bla DHA-1 (1.6%), and bla SHV-12 (1.6%), were found in FQ-resistant isolates. The results of PCR and direct sequencing showed that 37 isolates (14.9%) harbored plasmid-mediated quinolone resistance (PMQR) genes. qnrB2, qnrB4, qnrS1, coexistence of qnrB4 and qnrS1, oqxAB, and aac(6′)-Ib-cr were found in 1, 4, 4, 1, 15, and 14 isolates, respectively. PMQR genes were successfully transfered for 11 (29.7%) of the 37 PMQR-harboring isolates by conjugation to E. coli C600. These findings indicate that qnr genes remained rare in E. coli but demonstrate the potential spread of oqxAB and aac(6′)-Ib-c in Taiwan.

Original languageEnglish
Article number32281
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Aug 30

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Quinolones
Fluoroquinolones
Bacteremia
Taiwan
Plasmids
Escherichia coli
meropenem
Genes
Fosfomycin
Levofloxacin
Amikacin
Imipenem
Agar
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{b106f0ac5fec4f92901586ed9596ce4d,
title = "Plasmid-mediated quinolone resistance determinants in quinolone-resistant Escherichia coli isolated from patients with bacteremia in a university hospital in Taiwan, 2001-2015",
abstract = "The aim of this study was to characterize fluoroquinolone (FQ)-resistant Escherichia coli isolates from bacteremia in Taiwan in 2001-2015. During the study period, 248 (21.2{\%}) of 1171 isolates were identified as levofloxacin-resistant. The results of phylogenetic group analysis showed that 38.7{\%} of the FQ-resistant isolates belonged to phylogenetic group B2, 23.4{\%} to group B1, 22.6{\%} to groupA, 14.9{\%} to group D, and 0.4{\%} belonged to group F. FQ-resistant isolates were highly susceptible to cefepime (91.5{\%}), imipenem (96.0{\%}), meropenem (98.8{\%}), amikacin (98.0{\%}), and fosfomycin (99.6{\%}), as determined by the agar dilution method. β-lactamases, including bla TEM (66.1{\%}), bla CMY-2 (16.5{\%}), bla CTX-M (5.2{\%}), bla DHA-1 (1.6{\%}), and bla SHV-12 (1.6{\%}), were found in FQ-resistant isolates. The results of PCR and direct sequencing showed that 37 isolates (14.9{\%}) harbored plasmid-mediated quinolone resistance (PMQR) genes. qnrB2, qnrB4, qnrS1, coexistence of qnrB4 and qnrS1, oqxAB, and aac(6′)-Ib-cr were found in 1, 4, 4, 1, 15, and 14 isolates, respectively. PMQR genes were successfully transfered for 11 (29.7{\%}) of the 37 PMQR-harboring isolates by conjugation to E. coli C600. These findings indicate that qnr genes remained rare in E. coli but demonstrate the potential spread of oqxAB and aac(6′)-Ib-c in Taiwan.",
author = "Kao, {Cheng Yen} and Wu, {Hsiu Mei} and Wei-Hung Lin and Chin-Chung Tseng and Yan, {Jing Jou} and Ming-Cheng Wang and Ching-Hao Teng and Wu, {Jiunn Jong}",
year = "2016",
month = "8",
day = "30",
doi = "10.1038/srep32281",
language = "English",
volume = "6",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

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T1 - Plasmid-mediated quinolone resistance determinants in quinolone-resistant Escherichia coli isolated from patients with bacteremia in a university hospital in Taiwan, 2001-2015

AU - Kao, Cheng Yen

AU - Wu, Hsiu Mei

AU - Lin, Wei-Hung

AU - Tseng, Chin-Chung

AU - Yan, Jing Jou

AU - Wang, Ming-Cheng

AU - Teng, Ching-Hao

AU - Wu, Jiunn Jong

PY - 2016/8/30

Y1 - 2016/8/30

N2 - The aim of this study was to characterize fluoroquinolone (FQ)-resistant Escherichia coli isolates from bacteremia in Taiwan in 2001-2015. During the study period, 248 (21.2%) of 1171 isolates were identified as levofloxacin-resistant. The results of phylogenetic group analysis showed that 38.7% of the FQ-resistant isolates belonged to phylogenetic group B2, 23.4% to group B1, 22.6% to groupA, 14.9% to group D, and 0.4% belonged to group F. FQ-resistant isolates were highly susceptible to cefepime (91.5%), imipenem (96.0%), meropenem (98.8%), amikacin (98.0%), and fosfomycin (99.6%), as determined by the agar dilution method. β-lactamases, including bla TEM (66.1%), bla CMY-2 (16.5%), bla CTX-M (5.2%), bla DHA-1 (1.6%), and bla SHV-12 (1.6%), were found in FQ-resistant isolates. The results of PCR and direct sequencing showed that 37 isolates (14.9%) harbored plasmid-mediated quinolone resistance (PMQR) genes. qnrB2, qnrB4, qnrS1, coexistence of qnrB4 and qnrS1, oqxAB, and aac(6′)-Ib-cr were found in 1, 4, 4, 1, 15, and 14 isolates, respectively. PMQR genes were successfully transfered for 11 (29.7%) of the 37 PMQR-harboring isolates by conjugation to E. coli C600. These findings indicate that qnr genes remained rare in E. coli but demonstrate the potential spread of oqxAB and aac(6′)-Ib-c in Taiwan.

AB - The aim of this study was to characterize fluoroquinolone (FQ)-resistant Escherichia coli isolates from bacteremia in Taiwan in 2001-2015. During the study period, 248 (21.2%) of 1171 isolates were identified as levofloxacin-resistant. The results of phylogenetic group analysis showed that 38.7% of the FQ-resistant isolates belonged to phylogenetic group B2, 23.4% to group B1, 22.6% to groupA, 14.9% to group D, and 0.4% belonged to group F. FQ-resistant isolates were highly susceptible to cefepime (91.5%), imipenem (96.0%), meropenem (98.8%), amikacin (98.0%), and fosfomycin (99.6%), as determined by the agar dilution method. β-lactamases, including bla TEM (66.1%), bla CMY-2 (16.5%), bla CTX-M (5.2%), bla DHA-1 (1.6%), and bla SHV-12 (1.6%), were found in FQ-resistant isolates. The results of PCR and direct sequencing showed that 37 isolates (14.9%) harbored plasmid-mediated quinolone resistance (PMQR) genes. qnrB2, qnrB4, qnrS1, coexistence of qnrB4 and qnrS1, oqxAB, and aac(6′)-Ib-cr were found in 1, 4, 4, 1, 15, and 14 isolates, respectively. PMQR genes were successfully transfered for 11 (29.7%) of the 37 PMQR-harboring isolates by conjugation to E. coli C600. These findings indicate that qnr genes remained rare in E. coli but demonstrate the potential spread of oqxAB and aac(6′)-Ib-c in Taiwan.

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