Platelet-activating factor-acetylhydrolase (PLA2G7) A379V (exon 11) gene polymorphism is functionally associated with coronary artery disease severity but not the onset of acute coronary syndrome

Ping-Yen Liu, Hsing Chun Chung, Zi-Yi Chen, Cheng-Han Lee, Shih-Hung Chan, Yi-Heng Li, Li Jen Lin, Jyh Hong Chen

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Abstract

Background: Oxidation of low-density lipoproteins is an initial step of atherogenesis that generates proinflammatory phospholipids, including platelet-activating factor (PAF). PAF is degraded by PAF-acetylhydrolase (PAF-AH), which is also a risk factor for myocardial infarction. The role of PAF-AH in the onset of acute coronary syndrome (ACS) and its association with atherosclerosis among ACS patients are still unclear. Methods: PAF-AH encoding gene (PLA2G7) A379V variation was investigated in a cohort of patients having ACS (n = 200) and a sex-age-matched control group (n = 200). The activity of PAF-AH was evaluated by ELISA assay and coronary angiograms were evaluated among 100 ACS subjects for the severity of coronary atherosclerosis. Results: The V allele of A379V (exon 11) polymorphism on the PLA2G7 gene was more frequent in ACS patients (p = 0.02). This V allele polymorphism was also associated with a lower activity of plasma PAF-AH (VV vs. VA vs. AA: 9.8 ± 5.3 vs. 20.5 ± 7.7 vs. 24.8 ± 5.9 nmol/mL, respectively;p for trend = 0.03) and a more complex coronary atherosclerosis (diffuse score: VV vs. VA vs. AA: 7.3 ± 1.7 vs. 5.2 ± 1.4 vs. 3.4 ± 1.3, respectively;p for trend = 0.04). Multiple logistic regression analysis revealed three independent risk factors: smoking (OR 2.14, 95% CI 1.77 to 8.10, p = 0.02), diabetes mellitus (OR 2.08, 95% CI 1.55 to 5.32, p = 0.007) and hypertension (OR 3.18, 95% CI 1.15 to 7.36, p = 0.002), were all independent risk factors for the onset of ACS. However, this genetic variation did not show significant difference (OR 1.21, 95% CI 0.89 to 5.80, p = 0.18). Conclusion: We conclude that the PLA2G7/A379V polymorphism on exon 11 of PAF-AH gene is functionally associated with the PAF-AH activity and the severity of coronary atherosclerosis, but not onset of ACS, among Taiwanese population.

Original languageEnglish
Pages (from-to)212-220
Number of pages9
JournalActa Cardiologica Sinica
Volume22
Issue number4
Publication statusPublished - 2006 Dec 1

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Platelet Activating Factor
Acute Coronary Syndrome
Coronary Artery Disease
Exons
Genes
Atherosclerosis
Alleles
LDL Lipoproteins
Phospholipids
Diabetes Mellitus
Angiography
Research Design
Logistic Models
Smoking
Enzyme-Linked Immunosorbent Assay
Myocardial Infarction
Regression Analysis
Hypertension
Control Groups

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

Cite this

@article{0eb77dc7dfdf452d9a310a3e45f06eb2,
title = "Platelet-activating factor-acetylhydrolase (PLA2G7) A379V (exon 11) gene polymorphism is functionally associated with coronary artery disease severity but not the onset of acute coronary syndrome",
abstract = "Background: Oxidation of low-density lipoproteins is an initial step of atherogenesis that generates proinflammatory phospholipids, including platelet-activating factor (PAF). PAF is degraded by PAF-acetylhydrolase (PAF-AH), which is also a risk factor for myocardial infarction. The role of PAF-AH in the onset of acute coronary syndrome (ACS) and its association with atherosclerosis among ACS patients are still unclear. Methods: PAF-AH encoding gene (PLA2G7) A379V variation was investigated in a cohort of patients having ACS (n = 200) and a sex-age-matched control group (n = 200). The activity of PAF-AH was evaluated by ELISA assay and coronary angiograms were evaluated among 100 ACS subjects for the severity of coronary atherosclerosis. Results: The V allele of A379V (exon 11) polymorphism on the PLA2G7 gene was more frequent in ACS patients (p = 0.02). This V allele polymorphism was also associated with a lower activity of plasma PAF-AH (VV vs. VA vs. AA: 9.8 ± 5.3 vs. 20.5 ± 7.7 vs. 24.8 ± 5.9 nmol/mL, respectively;p for trend = 0.03) and a more complex coronary atherosclerosis (diffuse score: VV vs. VA vs. AA: 7.3 ± 1.7 vs. 5.2 ± 1.4 vs. 3.4 ± 1.3, respectively;p for trend = 0.04). Multiple logistic regression analysis revealed three independent risk factors: smoking (OR 2.14, 95{\%} CI 1.77 to 8.10, p = 0.02), diabetes mellitus (OR 2.08, 95{\%} CI 1.55 to 5.32, p = 0.007) and hypertension (OR 3.18, 95{\%} CI 1.15 to 7.36, p = 0.002), were all independent risk factors for the onset of ACS. However, this genetic variation did not show significant difference (OR 1.21, 95{\%} CI 0.89 to 5.80, p = 0.18). Conclusion: We conclude that the PLA2G7/A379V polymorphism on exon 11 of PAF-AH gene is functionally associated with the PAF-AH activity and the severity of coronary atherosclerosis, but not onset of ACS, among Taiwanese population.",
author = "Ping-Yen Liu and Chung, {Hsing Chun} and Zi-Yi Chen and Cheng-Han Lee and Shih-Hung Chan and Yi-Heng Li and Lin, {Li Jen} and Chen, {Jyh Hong}",
year = "2006",
month = "12",
day = "1",
language = "English",
volume = "22",
pages = "212--220",
journal = "Acta Cardiologica Sinica",
issn = "1011-6842",
publisher = "Republic of China Society of Cardiology",
number = "4",

}

TY - JOUR

T1 - Platelet-activating factor-acetylhydrolase (PLA2G7) A379V (exon 11) gene polymorphism is functionally associated with coronary artery disease severity but not the onset of acute coronary syndrome

AU - Liu, Ping-Yen

AU - Chung, Hsing Chun

AU - Chen, Zi-Yi

AU - Lee, Cheng-Han

AU - Chan, Shih-Hung

AU - Li, Yi-Heng

AU - Lin, Li Jen

AU - Chen, Jyh Hong

PY - 2006/12/1

Y1 - 2006/12/1

N2 - Background: Oxidation of low-density lipoproteins is an initial step of atherogenesis that generates proinflammatory phospholipids, including platelet-activating factor (PAF). PAF is degraded by PAF-acetylhydrolase (PAF-AH), which is also a risk factor for myocardial infarction. The role of PAF-AH in the onset of acute coronary syndrome (ACS) and its association with atherosclerosis among ACS patients are still unclear. Methods: PAF-AH encoding gene (PLA2G7) A379V variation was investigated in a cohort of patients having ACS (n = 200) and a sex-age-matched control group (n = 200). The activity of PAF-AH was evaluated by ELISA assay and coronary angiograms were evaluated among 100 ACS subjects for the severity of coronary atherosclerosis. Results: The V allele of A379V (exon 11) polymorphism on the PLA2G7 gene was more frequent in ACS patients (p = 0.02). This V allele polymorphism was also associated with a lower activity of plasma PAF-AH (VV vs. VA vs. AA: 9.8 ± 5.3 vs. 20.5 ± 7.7 vs. 24.8 ± 5.9 nmol/mL, respectively;p for trend = 0.03) and a more complex coronary atherosclerosis (diffuse score: VV vs. VA vs. AA: 7.3 ± 1.7 vs. 5.2 ± 1.4 vs. 3.4 ± 1.3, respectively;p for trend = 0.04). Multiple logistic regression analysis revealed three independent risk factors: smoking (OR 2.14, 95% CI 1.77 to 8.10, p = 0.02), diabetes mellitus (OR 2.08, 95% CI 1.55 to 5.32, p = 0.007) and hypertension (OR 3.18, 95% CI 1.15 to 7.36, p = 0.002), were all independent risk factors for the onset of ACS. However, this genetic variation did not show significant difference (OR 1.21, 95% CI 0.89 to 5.80, p = 0.18). Conclusion: We conclude that the PLA2G7/A379V polymorphism on exon 11 of PAF-AH gene is functionally associated with the PAF-AH activity and the severity of coronary atherosclerosis, but not onset of ACS, among Taiwanese population.

AB - Background: Oxidation of low-density lipoproteins is an initial step of atherogenesis that generates proinflammatory phospholipids, including platelet-activating factor (PAF). PAF is degraded by PAF-acetylhydrolase (PAF-AH), which is also a risk factor for myocardial infarction. The role of PAF-AH in the onset of acute coronary syndrome (ACS) and its association with atherosclerosis among ACS patients are still unclear. Methods: PAF-AH encoding gene (PLA2G7) A379V variation was investigated in a cohort of patients having ACS (n = 200) and a sex-age-matched control group (n = 200). The activity of PAF-AH was evaluated by ELISA assay and coronary angiograms were evaluated among 100 ACS subjects for the severity of coronary atherosclerosis. Results: The V allele of A379V (exon 11) polymorphism on the PLA2G7 gene was more frequent in ACS patients (p = 0.02). This V allele polymorphism was also associated with a lower activity of plasma PAF-AH (VV vs. VA vs. AA: 9.8 ± 5.3 vs. 20.5 ± 7.7 vs. 24.8 ± 5.9 nmol/mL, respectively;p for trend = 0.03) and a more complex coronary atherosclerosis (diffuse score: VV vs. VA vs. AA: 7.3 ± 1.7 vs. 5.2 ± 1.4 vs. 3.4 ± 1.3, respectively;p for trend = 0.04). Multiple logistic regression analysis revealed three independent risk factors: smoking (OR 2.14, 95% CI 1.77 to 8.10, p = 0.02), diabetes mellitus (OR 2.08, 95% CI 1.55 to 5.32, p = 0.007) and hypertension (OR 3.18, 95% CI 1.15 to 7.36, p = 0.002), were all independent risk factors for the onset of ACS. However, this genetic variation did not show significant difference (OR 1.21, 95% CI 0.89 to 5.80, p = 0.18). Conclusion: We conclude that the PLA2G7/A379V polymorphism on exon 11 of PAF-AH gene is functionally associated with the PAF-AH activity and the severity of coronary atherosclerosis, but not onset of ACS, among Taiwanese population.

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