Platelet factor 4 as a novel exosome marker in MALDI-MS analysis of exosomes from human serum

Huu Quang Nguyen, Dabin Lee, Yeoseon Kim, Minseok Paek, Minsun Kim, Kyoung Soon Jang, Jooyeon Oh, Young Sun Lee, Jong Eun Yeon, David M. Lubman, Jeongkwon Kim

Research output: Contribution to journalArticle

Abstract

Exosomes are nanosized vesicles commonly found in biological fluids as a result of a secretion process involving endosomes and multivesicular bodies. The isolation and analysis of exosomes can be useful for noninvasive clinical diagnosis of a variety of human diseases. We investigated the utility of analyzing exosomal proteins, using matrix-assisted laser desorption/ionization combined with Fourier-transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS), as a means of determining the presence of exosomes. MALDI-FTICR-MS analyses of exosomes enriched from human serum via centrifugation in a mass range of m/z 1000-20 »000 yielded a distinctive protein around m/z 7766. The high mass accuracy and resolution of MALDI-FTICR-MS allowed for reliable comparisons against a protein database, through which the protein was identified as platelet factor 4 (PLF4), whose singly charged protein peak has an elemental composition of C341H577N96O101S4 +, with a theoretical most abundant isotopic peak at m/z 7765.194 and a theoretical average peak at m/z 7766. The MALDI-TOF MS analysis of exosomes from the serum of 27 patients with different states of liver diseases provided the most abundant PLF4 peak for each mass spectrum, along with several additional minor peaks. In conclusion, MALDI-MS is suitable as an alternative exosome detection method, serving as a valuable confirmation tool, greatly decreasing the time and workload associated with exosome identification. ©

Original languageEnglish
JournalAnalytical chemistry
DOIs
Publication statusAccepted/In press - 2019 Jan 1

Fingerprint

Platelet Factor 4
Cyclotron resonance
Ionization
Mass spectrometry
Desorption
Fourier transforms
Ions
Proteins
Lasers
Centrifugation
Liver
Fluids
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Nguyen, Huu Quang ; Lee, Dabin ; Kim, Yeoseon ; Paek, Minseok ; Kim, Minsun ; Jang, Kyoung Soon ; Oh, Jooyeon ; Lee, Young Sun ; Yeon, Jong Eun ; Lubman, David M. ; Kim, Jeongkwon. / Platelet factor 4 as a novel exosome marker in MALDI-MS analysis of exosomes from human serum. In: Analytical chemistry. 2019.
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abstract = "Exosomes are nanosized vesicles commonly found in biological fluids as a result of a secretion process involving endosomes and multivesicular bodies. The isolation and analysis of exosomes can be useful for noninvasive clinical diagnosis of a variety of human diseases. We investigated the utility of analyzing exosomal proteins, using matrix-assisted laser desorption/ionization combined with Fourier-transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS), as a means of determining the presence of exosomes. MALDI-FTICR-MS analyses of exosomes enriched from human serum via centrifugation in a mass range of m/z 1000-20 »000 yielded a distinctive protein around m/z 7766. The high mass accuracy and resolution of MALDI-FTICR-MS allowed for reliable comparisons against a protein database, through which the protein was identified as platelet factor 4 (PLF4), whose singly charged protein peak has an elemental composition of C341H577N96O101S4 +, with a theoretical most abundant isotopic peak at m/z 7765.194 and a theoretical average peak at m/z 7766. The MALDI-TOF MS analysis of exosomes from the serum of 27 patients with different states of liver diseases provided the most abundant PLF4 peak for each mass spectrum, along with several additional minor peaks. In conclusion, MALDI-MS is suitable as an alternative exosome detection method, serving as a valuable confirmation tool, greatly decreasing the time and workload associated with exosome identification. {\circledC}",
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Platelet factor 4 as a novel exosome marker in MALDI-MS analysis of exosomes from human serum. / Nguyen, Huu Quang; Lee, Dabin; Kim, Yeoseon; Paek, Minseok; Kim, Minsun; Jang, Kyoung Soon; Oh, Jooyeon; Lee, Young Sun; Yeon, Jong Eun; Lubman, David M.; Kim, Jeongkwon.

In: Analytical chemistry, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Platelet factor 4 as a novel exosome marker in MALDI-MS analysis of exosomes from human serum

AU - Nguyen, Huu Quang

AU - Lee, Dabin

AU - Kim, Yeoseon

AU - Paek, Minseok

AU - Kim, Minsun

AU - Jang, Kyoung Soon

AU - Oh, Jooyeon

AU - Lee, Young Sun

AU - Yeon, Jong Eun

AU - Lubman, David M.

AU - Kim, Jeongkwon

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Exosomes are nanosized vesicles commonly found in biological fluids as a result of a secretion process involving endosomes and multivesicular bodies. The isolation and analysis of exosomes can be useful for noninvasive clinical diagnosis of a variety of human diseases. We investigated the utility of analyzing exosomal proteins, using matrix-assisted laser desorption/ionization combined with Fourier-transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS), as a means of determining the presence of exosomes. MALDI-FTICR-MS analyses of exosomes enriched from human serum via centrifugation in a mass range of m/z 1000-20 »000 yielded a distinctive protein around m/z 7766. The high mass accuracy and resolution of MALDI-FTICR-MS allowed for reliable comparisons against a protein database, through which the protein was identified as platelet factor 4 (PLF4), whose singly charged protein peak has an elemental composition of C341H577N96O101S4 +, with a theoretical most abundant isotopic peak at m/z 7765.194 and a theoretical average peak at m/z 7766. The MALDI-TOF MS analysis of exosomes from the serum of 27 patients with different states of liver diseases provided the most abundant PLF4 peak for each mass spectrum, along with several additional minor peaks. In conclusion, MALDI-MS is suitable as an alternative exosome detection method, serving as a valuable confirmation tool, greatly decreasing the time and workload associated with exosome identification. ©

AB - Exosomes are nanosized vesicles commonly found in biological fluids as a result of a secretion process involving endosomes and multivesicular bodies. The isolation and analysis of exosomes can be useful for noninvasive clinical diagnosis of a variety of human diseases. We investigated the utility of analyzing exosomal proteins, using matrix-assisted laser desorption/ionization combined with Fourier-transform ion cyclotron resonance mass spectrometry (MALDI-FTICR-MS), as a means of determining the presence of exosomes. MALDI-FTICR-MS analyses of exosomes enriched from human serum via centrifugation in a mass range of m/z 1000-20 »000 yielded a distinctive protein around m/z 7766. The high mass accuracy and resolution of MALDI-FTICR-MS allowed for reliable comparisons against a protein database, through which the protein was identified as platelet factor 4 (PLF4), whose singly charged protein peak has an elemental composition of C341H577N96O101S4 +, with a theoretical most abundant isotopic peak at m/z 7765.194 and a theoretical average peak at m/z 7766. The MALDI-TOF MS analysis of exosomes from the serum of 27 patients with different states of liver diseases provided the most abundant PLF4 peak for each mass spectrum, along with several additional minor peaks. In conclusion, MALDI-MS is suitable as an alternative exosome detection method, serving as a valuable confirmation tool, greatly decreasing the time and workload associated with exosome identification. ©

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