TY - JOUR
T1 - Polyhydroxybutyrate (PHB) production from crude glycerol by genetic engineering of Rhodotorula glutinis
AU - Aristya, Ganies Riza
AU - Lin, Yu Ju
AU - Chang, Jo Shu
AU - Chang, Jui Jen
AU - Yen, Hong Wei
N1 - Funding Information:
The authors gratefully acknowledge the financial support received for this study from Taiwan's Ministry of Science and Technology ( MOST ) under grant numbers MOST 107-2621-M-029-001 . The author (Ganies Riza Aristya) wishes to thank the Taiwan's Ministry of Education for the scholarship support during the study at Tunghai University, Taiwan.
Funding Information:
The authors gratefully acknowledge the financial support received for this study from Taiwan's Ministry of Science and Technology (MOST) under grant numbers MOST 107-2621-M-029-001. The author (Ganies Riza Aristya) wishes to thank the Taiwan's Ministry of Education for the scholarship support during the study at Tunghai University, Taiwan.
Publisher Copyright:
© 2022 Elsevier Ltd
PY - 2022/6
Y1 - 2022/6
N2 - Polyhydroxybutyrate (PHB) is a member of the Polyhydroxyalkanoates (PHAs) family, which is generally produced through microbial biosynthesis. The synthetic biology tools are applied to transform PHB genes (PhaA, PhaB and PhaC) into an oleaginous yeast (Rhodotorula glutinis) genome. Western blot analysis confirms the expression of PhaA, PhaB and PhaC in the candidate transformants. The strain of R. glutinis #100–29 was selected to be the best PHB production candidate according to the shaker results. As compared to glucose, crude glycerol was known to be a suitable carbon source for R. glutinis cultivation. The PHB concentration, yield and content obtained in a 5-L fermenter using 30 g/L of crude glycerol were 2.87 g/L, 95.53 mg/g and 62% respectively. To the best of our knowledge, these should be the highest numbers obtained in yeast for PHB production, which indicates that strain #100–29 could be a potential PHB producer from crude glycerol.
AB - Polyhydroxybutyrate (PHB) is a member of the Polyhydroxyalkanoates (PHAs) family, which is generally produced through microbial biosynthesis. The synthetic biology tools are applied to transform PHB genes (PhaA, PhaB and PhaC) into an oleaginous yeast (Rhodotorula glutinis) genome. Western blot analysis confirms the expression of PhaA, PhaB and PhaC in the candidate transformants. The strain of R. glutinis #100–29 was selected to be the best PHB production candidate according to the shaker results. As compared to glucose, crude glycerol was known to be a suitable carbon source for R. glutinis cultivation. The PHB concentration, yield and content obtained in a 5-L fermenter using 30 g/L of crude glycerol were 2.87 g/L, 95.53 mg/g and 62% respectively. To the best of our knowledge, these should be the highest numbers obtained in yeast for PHB production, which indicates that strain #100–29 could be a potential PHB producer from crude glycerol.
UR - https://www.scopus.com/pages/publications/85128231518
UR - https://www.scopus.com/pages/publications/85128231518#tab=citedBy
U2 - 10.1016/j.biteb.2022.101048
DO - 10.1016/j.biteb.2022.101048
M3 - Article
AN - SCOPUS:85128231518
SN - 2589-014X
VL - 18
JO - Bioresource Technology Reports
JF - Bioresource Technology Reports
M1 - 101048
ER -