Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer

D. Y. Lin; T. Y. Chiang; C. C. Huang; H. D. Lin; S. J. Tzeng; S. R. Kang; H. M. Sung; M. C. Wu

doi:10.4238/2014.May.23.7

Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer

D. Y. Lin, T. Y. Chiang, C. C. Huang, H. D. Lin, S. J. Tzeng, S. R. Kang, H. M. Sung, M. C. Wu

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg equilibrium due to excessive homozygosity in these domesticated broodstocks, reflecting inbreeding. These microsatellite loci will be useful, not only for assessment of population structure and genetic variability, but also for conservation of wild deer populations in Taiwan.

Original language	English
Pages (from-to)	3967-3971
Number of pages	5
Journal	Genetics and Molecular Research
Volume	13
Issue number	2
DOIs	https://doi.org/10.4238/2014.May.23.7
Publication status	Published - 2014 May 23

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Access to Document

10.4238/2014.May.23.7

Cite this

@article{04a69b2235c34477a2881685c6a9581e,

title = "Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer",

abstract = "Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg equilibrium due to excessive homozygosity in these domesticated broodstocks, reflecting inbreeding. These microsatellite loci will be useful, not only for assessment of population structure and genetic variability, but also for conservation of wild deer populations in Taiwan.",

author = "Lin, {D. Y.} and Chiang, {T. Y.} and Huang, {C. C.} and Lin, {H. D.} and Tzeng, {S. J.} and Kang, {S. R.} and Sung, {H. M.} and Wu, {M. C.}",

year = "2014",

month = may,

day = "23",

doi = "10.4238/2014.May.23.7",

language = "English",

volume = "13",

pages = "3967--3971",

journal = "Genetics and Molecular Research",

issn = "1676-5680",

publisher = "Fundacao de Pesquisas Cientificas de Ribeirao Preto",

number = "2",

}

TY - JOUR

T1 - Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer

AU - Lin, D. Y.

AU - Chiang, T. Y.

AU - Huang, C. C.

AU - Lin, H. D.

AU - Tzeng, S. J.

AU - Kang, S. R.

AU - Sung, H. M.

AU - Wu, M. C.

PY - 2014/5/23

Y1 - 2014/5/23

N2 - Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg equilibrium due to excessive homozygosity in these domesticated broodstocks, reflecting inbreeding. These microsatellite loci will be useful, not only for assessment of population structure and genetic variability, but also for conservation of wild deer populations in Taiwan.

AB - Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci but one, CU18, deviated from Hardy-Weinberg equilibrium due to excessive homozygosity in these domesticated broodstocks, reflecting inbreeding. These microsatellite loci will be useful, not only for assessment of population structure and genetic variability, but also for conservation of wild deer populations in Taiwan.

UR - http://www.scopus.com/inward/record.url?scp=84901423112&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901423112&partnerID=8YFLogxK

U2 - 10.4238/2014.May.23.7

DO - 10.4238/2014.May.23.7

M3 - Article

C2 - 24938607

AN - SCOPUS:84901423112

SN - 1676-5680

VL - 13

SP - 3967

EP - 3971

JO - Genetics and Molecular Research

JF - Genetics and Molecular Research

IS - 2

ER -

Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this