Positively charged GFP-chromophore-based fluorescent sensor for DNA

Ming Ju Li; Jun Jia Xu; Robert Sung; Kuangsen Sung

doi:10.1016/j.optmat.2024.115581

Positively charged GFP-chromophore-based fluorescent sensor for DNA

Ming Ju Li, Jun Jia Xu, Robert Sung, Kuangsen Sung

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Fluorescence titration of the positively charged green fluorescent protein (GFP) chromophore analogue 1 with E. Coli plasmid DNA [pcDNA3.1(−)] confirms that 1 serves as a fluorescent probe for sensing E. Coli plasmid DNA. The binding constant K between the DNA and 1 is as large as 2.9 × 10⁵. The sensing mechanism is that the intermolecular steric hindrance generated between 1 and E. Coli plasmid DNA by electrostatic attraction restricts the φ-torsion around P-bond and τ-torsion around I-bond of 1, enhancing fluorescence of 1.

Original language	English
Article number	115581
Journal	Optical Materials
Volume	153
DOIs	https://doi.org/10.1016/j.optmat.2024.115581
Publication status	Published - 2024 Jul

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Spectroscopy
Physical and Theoretical Chemistry
Organic Chemistry
Inorganic Chemistry
Electrical and Electronic Engineering

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10.1016/j.optmat.2024.115581

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title = "Positively charged GFP-chromophore-based fluorescent sensor for DNA",

abstract = "Fluorescence titration of the positively charged green fluorescent protein (GFP) chromophore analogue 1 with E. Coli plasmid DNA [pcDNA3.1(−)] confirms that 1 serves as a fluorescent probe for sensing E. Coli plasmid DNA. The binding constant K between the DNA and 1 is as large as 2.9 × 105. The sensing mechanism is that the intermolecular steric hindrance generated between 1 and E. Coli plasmid DNA by electrostatic attraction restricts the φ-torsion around P-bond and τ-torsion around I-bond of 1, enhancing fluorescence of 1.",

author = "Li, {Ming Ju} and Xu, {Jun Jia} and Robert Sung and Kuangsen Sung",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2024",

month = jul,

doi = "10.1016/j.optmat.2024.115581",

language = "English",

volume = "153",

journal = "Optical Materials",

issn = "0925-3467",

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T1 - Positively charged GFP-chromophore-based fluorescent sensor for DNA

AU - Li, Ming Ju

AU - Xu, Jun Jia

AU - Sung, Robert

AU - Sung, Kuangsen

PY - 2024/7

Y1 - 2024/7

N2 - Fluorescence titration of the positively charged green fluorescent protein (GFP) chromophore analogue 1 with E. Coli plasmid DNA [pcDNA3.1(−)] confirms that 1 serves as a fluorescent probe for sensing E. Coli plasmid DNA. The binding constant K between the DNA and 1 is as large as 2.9 × 105. The sensing mechanism is that the intermolecular steric hindrance generated between 1 and E. Coli plasmid DNA by electrostatic attraction restricts the φ-torsion around P-bond and τ-torsion around I-bond of 1, enhancing fluorescence of 1.

AB - Fluorescence titration of the positively charged green fluorescent protein (GFP) chromophore analogue 1 with E. Coli plasmid DNA [pcDNA3.1(−)] confirms that 1 serves as a fluorescent probe for sensing E. Coli plasmid DNA. The binding constant K between the DNA and 1 is as large as 2.9 × 105. The sensing mechanism is that the intermolecular steric hindrance generated between 1 and E. Coli plasmid DNA by electrostatic attraction restricts the φ-torsion around P-bond and τ-torsion around I-bond of 1, enhancing fluorescence of 1.

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M3 - Article

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SN - 0925-3467

VL - 153

JO - Optical Materials

JF - Optical Materials

M1 - 115581

ER -

Positively charged GFP-chromophore-based fluorescent sensor for DNA

Abstract

All Science Journal Classification (ASJC) codes

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