Possible biphasic changes of free radicals in ethylene glycol-induced nephrolithiasis in rats

Ho-Shiang Huang, C. F. Chen, C. T. Chien, J. Chen

Research output: Contribution to journalArticle

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Abstract

Objective. To evaluate the possible role of free radicals in nephrolithiasis in rats induced by ethylene glycol, and to examine the correlation between the urinary enzymes N-acetyl-β-glucosaminidase (NAG), β-galactosidase (GAL) and neutral endopeptidase (NEP), and free radical production. Materials and methods. Hyperoxaluria was produced in male Wistar rats by adding ethylene glycol to their drinking water. After 7, 21 and 42 days of treatment, urinary oxalate, creatinine clearance and urinary enzymes (NAG, GAL and NEP) were measured. The nitroblue tetrazolium perfusion method was used to locate the sites of free-radical production. Ultrasensitive chemiluminescence was used to directly measure the production of reactive oxygen species (ROS) in vivo. Vitamin E and potassium citrate were fed to rats, in addition to ethylene glycol, to assess their effects on free radical production. Results. Urinary oxalate increased significantly and was associated with an increase in NAG and GAL at all sample times. However, urinary NEP activity was unchanged on day 7, although there was four times as much NEP on days 21 and 42 than in the control groups. Formazan particles in the renal cortex were scored as 3+ to 4+ in rats treated for 7 days with ethylene glycol. Blood ROS levels were also higher in this group than in the controls (P < 0.01). After Vitamin E and potassium citrate treatment, blood ROS levels were lower than in rats treated with ethylene glycol alone. Conclusion. Free radicals may be produced in the early stages of nephrolithiasis in rats fed with ethylene glycol. Free radicals occurred mainly in blood and might be associated with NEP inactivation.

Original languageEnglish
Pages (from-to)1143-1149
Number of pages7
JournalBJU International
Volume85
Issue number9
DOIs
Publication statusPublished - 2000 Jun 24

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Nephrolithiasis
Ethylene Glycol
Neprilysin
Free Radicals
Galactosidases
Hexosaminidases
Potassium Citrate
Reactive Oxygen Species
Oxalates
Vitamin E
Formazans
Hyperoxaluria
Nitroblue Tetrazolium
Control Groups
Enzymes
Luminescence
Drinking Water
Wistar Rats
Creatinine
Perfusion

All Science Journal Classification (ASJC) codes

  • Urology

Cite this

Huang, Ho-Shiang ; Chen, C. F. ; Chien, C. T. ; Chen, J. / Possible biphasic changes of free radicals in ethylene glycol-induced nephrolithiasis in rats. In: BJU International. 2000 ; Vol. 85, No. 9. pp. 1143-1149.
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abstract = "Objective. To evaluate the possible role of free radicals in nephrolithiasis in rats induced by ethylene glycol, and to examine the correlation between the urinary enzymes N-acetyl-β-glucosaminidase (NAG), β-galactosidase (GAL) and neutral endopeptidase (NEP), and free radical production. Materials and methods. Hyperoxaluria was produced in male Wistar rats by adding ethylene glycol to their drinking water. After 7, 21 and 42 days of treatment, urinary oxalate, creatinine clearance and urinary enzymes (NAG, GAL and NEP) were measured. The nitroblue tetrazolium perfusion method was used to locate the sites of free-radical production. Ultrasensitive chemiluminescence was used to directly measure the production of reactive oxygen species (ROS) in vivo. Vitamin E and potassium citrate were fed to rats, in addition to ethylene glycol, to assess their effects on free radical production. Results. Urinary oxalate increased significantly and was associated with an increase in NAG and GAL at all sample times. However, urinary NEP activity was unchanged on day 7, although there was four times as much NEP on days 21 and 42 than in the control groups. Formazan particles in the renal cortex were scored as 3+ to 4+ in rats treated for 7 days with ethylene glycol. Blood ROS levels were also higher in this group than in the controls (P < 0.01). After Vitamin E and potassium citrate treatment, blood ROS levels were lower than in rats treated with ethylene glycol alone. Conclusion. Free radicals may be produced in the early stages of nephrolithiasis in rats fed with ethylene glycol. Free radicals occurred mainly in blood and might be associated with NEP inactivation.",
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Possible biphasic changes of free radicals in ethylene glycol-induced nephrolithiasis in rats. / Huang, Ho-Shiang; Chen, C. F.; Chien, C. T.; Chen, J.

In: BJU International, Vol. 85, No. 9, 24.06.2000, p. 1143-1149.

Research output: Contribution to journalArticle

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AU - Chien, C. T.

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N2 - Objective. To evaluate the possible role of free radicals in nephrolithiasis in rats induced by ethylene glycol, and to examine the correlation between the urinary enzymes N-acetyl-β-glucosaminidase (NAG), β-galactosidase (GAL) and neutral endopeptidase (NEP), and free radical production. Materials and methods. Hyperoxaluria was produced in male Wistar rats by adding ethylene glycol to their drinking water. After 7, 21 and 42 days of treatment, urinary oxalate, creatinine clearance and urinary enzymes (NAG, GAL and NEP) were measured. The nitroblue tetrazolium perfusion method was used to locate the sites of free-radical production. Ultrasensitive chemiluminescence was used to directly measure the production of reactive oxygen species (ROS) in vivo. Vitamin E and potassium citrate were fed to rats, in addition to ethylene glycol, to assess their effects on free radical production. Results. Urinary oxalate increased significantly and was associated with an increase in NAG and GAL at all sample times. However, urinary NEP activity was unchanged on day 7, although there was four times as much NEP on days 21 and 42 than in the control groups. Formazan particles in the renal cortex were scored as 3+ to 4+ in rats treated for 7 days with ethylene glycol. Blood ROS levels were also higher in this group than in the controls (P < 0.01). After Vitamin E and potassium citrate treatment, blood ROS levels were lower than in rats treated with ethylene glycol alone. Conclusion. Free radicals may be produced in the early stages of nephrolithiasis in rats fed with ethylene glycol. Free radicals occurred mainly in blood and might be associated with NEP inactivation.

AB - Objective. To evaluate the possible role of free radicals in nephrolithiasis in rats induced by ethylene glycol, and to examine the correlation between the urinary enzymes N-acetyl-β-glucosaminidase (NAG), β-galactosidase (GAL) and neutral endopeptidase (NEP), and free radical production. Materials and methods. Hyperoxaluria was produced in male Wistar rats by adding ethylene glycol to their drinking water. After 7, 21 and 42 days of treatment, urinary oxalate, creatinine clearance and urinary enzymes (NAG, GAL and NEP) were measured. The nitroblue tetrazolium perfusion method was used to locate the sites of free-radical production. Ultrasensitive chemiluminescence was used to directly measure the production of reactive oxygen species (ROS) in vivo. Vitamin E and potassium citrate were fed to rats, in addition to ethylene glycol, to assess their effects on free radical production. Results. Urinary oxalate increased significantly and was associated with an increase in NAG and GAL at all sample times. However, urinary NEP activity was unchanged on day 7, although there was four times as much NEP on days 21 and 42 than in the control groups. Formazan particles in the renal cortex were scored as 3+ to 4+ in rats treated for 7 days with ethylene glycol. Blood ROS levels were also higher in this group than in the controls (P < 0.01). After Vitamin E and potassium citrate treatment, blood ROS levels were lower than in rats treated with ethylene glycol alone. Conclusion. Free radicals may be produced in the early stages of nephrolithiasis in rats fed with ethylene glycol. Free radicals occurred mainly in blood and might be associated with NEP inactivation.

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