Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants

Po Yu Chen, Kuo Ting Lee, Wen Chang Chi, Heribert Hirt, Ching Chun Chang, Hao Jen Huang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Cross tolerance is a phenomenon that occurs when a plant, in resisting one form of stress, develops a tolerance to another form. Pretreatment with nonlethal heat shock has been known to protect cells from metal stress. In this study, we found that the treatment of rice roots with more than 25 μM of Cu2+ caused cell death. However, heat shock pretreatment attenuated Cu2+-induced cell death. The mechanisms of the cross tolerance phenomenon between heat shock and Cu2+ stress were investigated by pretreated rice roots with the protein synthesis inhibitor cycloheximide (CHX). CHX effectively block heat shock protection, suggesting that protection of Cu2+-induced cell death by heat shock was dependent on de novo protein synthesis. In addition, heat pretreatment downregulated ROS production and mitogen-activated protein kinase (MAPK) activities, both of which can be greatly elicited by Cu2+ stress in rice roots. Moreover, the addition of purified recombinant GST-OsHSP70 fusion proteins inhibited Cu 2+-enhanced MAPK activities in an in vitro kinase assay. Furthermore, loss of heat shock protection was observed in Arabidopsis mkk2 and mpk6 but not in mpk3 mutants under Cu2+ stress. Taken together, these results suggest that the interaction of OsHSP70 with MAPKs may contribute to the cellular protection in rice roots from excessive Cu2+ toxicity.

Original languageEnglish
Pages (from-to)499-509
Number of pages11
JournalPlanta
Volume228
Issue number3
DOIs
Publication statusPublished - 2008 Aug 1

Fingerprint

metal tolerance
mitogen-activated protein kinase
heat stress
Phosphotransferases
Hot Temperature
Metals
Shock
heat
cell death
rice
pretreatment
cycloheximide
Cell Death
Cycloheximide
protein synthesis inhibitors
Protein Synthesis Inhibitors
Mitogen-Activated Protein Kinase 1
stress tolerance
Thermotolerance
Mitogen-Activated Protein Kinases

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Chen, Po Yu ; Lee, Kuo Ting ; Chi, Wen Chang ; Hirt, Heribert ; Chang, Ching Chun ; Huang, Hao Jen. / Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants. In: Planta. 2008 ; Vol. 228, No. 3. pp. 499-509.
@article{4e333c2d426a4af2a30bd58caa62df08,
title = "Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants",
abstract = "Cross tolerance is a phenomenon that occurs when a plant, in resisting one form of stress, develops a tolerance to another form. Pretreatment with nonlethal heat shock has been known to protect cells from metal stress. In this study, we found that the treatment of rice roots with more than 25 μM of Cu2+ caused cell death. However, heat shock pretreatment attenuated Cu2+-induced cell death. The mechanisms of the cross tolerance phenomenon between heat shock and Cu2+ stress were investigated by pretreated rice roots with the protein synthesis inhibitor cycloheximide (CHX). CHX effectively block heat shock protection, suggesting that protection of Cu2+-induced cell death by heat shock was dependent on de novo protein synthesis. In addition, heat pretreatment downregulated ROS production and mitogen-activated protein kinase (MAPK) activities, both of which can be greatly elicited by Cu2+ stress in rice roots. Moreover, the addition of purified recombinant GST-OsHSP70 fusion proteins inhibited Cu 2+-enhanced MAPK activities in an in vitro kinase assay. Furthermore, loss of heat shock protection was observed in Arabidopsis mkk2 and mpk6 but not in mpk3 mutants under Cu2+ stress. Taken together, these results suggest that the interaction of OsHSP70 with MAPKs may contribute to the cellular protection in rice roots from excessive Cu2+ toxicity.",
author = "Chen, {Po Yu} and Lee, {Kuo Ting} and Chi, {Wen Chang} and Heribert Hirt and Chang, {Ching Chun} and Huang, {Hao Jen}",
year = "2008",
month = "8",
day = "1",
doi = "10.1007/s00425-008-0753-x",
language = "English",
volume = "228",
pages = "499--509",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Verlag",
number = "3",

}

Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants. / Chen, Po Yu; Lee, Kuo Ting; Chi, Wen Chang; Hirt, Heribert; Chang, Ching Chun; Huang, Hao Jen.

In: Planta, Vol. 228, No. 3, 01.08.2008, p. 499-509.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Possible involvement of MAP kinase pathways in acquired metal-tolerance induced by heat in plants

AU - Chen, Po Yu

AU - Lee, Kuo Ting

AU - Chi, Wen Chang

AU - Hirt, Heribert

AU - Chang, Ching Chun

AU - Huang, Hao Jen

PY - 2008/8/1

Y1 - 2008/8/1

N2 - Cross tolerance is a phenomenon that occurs when a plant, in resisting one form of stress, develops a tolerance to another form. Pretreatment with nonlethal heat shock has been known to protect cells from metal stress. In this study, we found that the treatment of rice roots with more than 25 μM of Cu2+ caused cell death. However, heat shock pretreatment attenuated Cu2+-induced cell death. The mechanisms of the cross tolerance phenomenon between heat shock and Cu2+ stress were investigated by pretreated rice roots with the protein synthesis inhibitor cycloheximide (CHX). CHX effectively block heat shock protection, suggesting that protection of Cu2+-induced cell death by heat shock was dependent on de novo protein synthesis. In addition, heat pretreatment downregulated ROS production and mitogen-activated protein kinase (MAPK) activities, both of which can be greatly elicited by Cu2+ stress in rice roots. Moreover, the addition of purified recombinant GST-OsHSP70 fusion proteins inhibited Cu 2+-enhanced MAPK activities in an in vitro kinase assay. Furthermore, loss of heat shock protection was observed in Arabidopsis mkk2 and mpk6 but not in mpk3 mutants under Cu2+ stress. Taken together, these results suggest that the interaction of OsHSP70 with MAPKs may contribute to the cellular protection in rice roots from excessive Cu2+ toxicity.

AB - Cross tolerance is a phenomenon that occurs when a plant, in resisting one form of stress, develops a tolerance to another form. Pretreatment with nonlethal heat shock has been known to protect cells from metal stress. In this study, we found that the treatment of rice roots with more than 25 μM of Cu2+ caused cell death. However, heat shock pretreatment attenuated Cu2+-induced cell death. The mechanisms of the cross tolerance phenomenon between heat shock and Cu2+ stress were investigated by pretreated rice roots with the protein synthesis inhibitor cycloheximide (CHX). CHX effectively block heat shock protection, suggesting that protection of Cu2+-induced cell death by heat shock was dependent on de novo protein synthesis. In addition, heat pretreatment downregulated ROS production and mitogen-activated protein kinase (MAPK) activities, both of which can be greatly elicited by Cu2+ stress in rice roots. Moreover, the addition of purified recombinant GST-OsHSP70 fusion proteins inhibited Cu 2+-enhanced MAPK activities in an in vitro kinase assay. Furthermore, loss of heat shock protection was observed in Arabidopsis mkk2 and mpk6 but not in mpk3 mutants under Cu2+ stress. Taken together, these results suggest that the interaction of OsHSP70 with MAPKs may contribute to the cellular protection in rice roots from excessive Cu2+ toxicity.

UR - http://www.scopus.com/inward/record.url?scp=47249131585&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47249131585&partnerID=8YFLogxK

U2 - 10.1007/s00425-008-0753-x

DO - 10.1007/s00425-008-0753-x

M3 - Article

C2 - 18506480

AN - SCOPUS:47249131585

VL - 228

SP - 499

EP - 509

JO - Planta

JF - Planta

SN - 0032-0935

IS - 3

ER -