Postoperative immuno-gene therapy of murine bladder tumor by in vivo administration of retroviruses expressing mouse interferon-γ

Ai-Li Shiau, Chih Yun Lin, Tzong Shin Tzai, Chao-Liang Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The murine MBT-2 bladder tumor model in syngeneic C3H/HeN mice was used to investigate the feasibility of gene therapy based on the delivery of interferon-γ (IFN-γ) in vivo by retroviral vectors. We constructed a recombinant retroviral vector pRUFneo/IFN-γ, which was transfected into a retroviral packaging cell line ψCRE, to produce ψCRE/pRUFneo/IFN-γ cells. The expressions of the neo and IFN-γ genes were verified by reverse transcription-polymerase chain reaction and IFN-γ was detected in the culture supernatant from ψCRE/pRUFneo/IFN-γ cells. After receiving MBT-2 cells admixed with retroviral pRUFneoIFN-γ supernatant, C3H/HeN mice exhibited lower tumor incidence, lower tumor mass, and higher survival rate, as well as higher antitumor responses compared to those injected with MBT-2 cells admixed with control retroviral supernatant. Moreover, the retroviral pRUFneoIFN-γ supernatant was able to suppress the growth of rechallenged tumors in postoperated mice. Although the IFN-γ protein secreted from ψCRE/pRUFneo/IFN-γ cells partly contributes to the antitumor effect of retroviral pRUFneoIFN-γ supernatant, the retroviruses carrying the IFN-γ gene transduced MBT-2 cells in vivo, which may result in enhancing local IFN-γ production from tumor cells. Because bladder is suitable for the intravesical instillation of therapeutic agents, in vivo administration of retroviral vectors encoding IFN-γ may be explored for the treatment of bladder cancer.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalCancer Gene Therapy
Volume8
Issue number1
DOIs
Publication statusPublished - 2001 Jan 1

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Retroviridae
Urinary Bladder Neoplasms
Genetic Therapy
Interferons
Inbred C3H Mouse
Neoplasms
Intravesical Administration
Product Packaging
Genes
Reverse Transcription
Urinary Bladder
Cell Line
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Cancer Research

Cite this

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title = "Postoperative immuno-gene therapy of murine bladder tumor by in vivo administration of retroviruses expressing mouse interferon-γ",
abstract = "The murine MBT-2 bladder tumor model in syngeneic C3H/HeN mice was used to investigate the feasibility of gene therapy based on the delivery of interferon-γ (IFN-γ) in vivo by retroviral vectors. We constructed a recombinant retroviral vector pRUFneo/IFN-γ, which was transfected into a retroviral packaging cell line ψCRE, to produce ψCRE/pRUFneo/IFN-γ cells. The expressions of the neo and IFN-γ genes were verified by reverse transcription-polymerase chain reaction and IFN-γ was detected in the culture supernatant from ψCRE/pRUFneo/IFN-γ cells. After receiving MBT-2 cells admixed with retroviral pRUFneoIFN-γ supernatant, C3H/HeN mice exhibited lower tumor incidence, lower tumor mass, and higher survival rate, as well as higher antitumor responses compared to those injected with MBT-2 cells admixed with control retroviral supernatant. Moreover, the retroviral pRUFneoIFN-γ supernatant was able to suppress the growth of rechallenged tumors in postoperated mice. Although the IFN-γ protein secreted from ψCRE/pRUFneo/IFN-γ cells partly contributes to the antitumor effect of retroviral pRUFneoIFN-γ supernatant, the retroviruses carrying the IFN-γ gene transduced MBT-2 cells in vivo, which may result in enhancing local IFN-γ production from tumor cells. Because bladder is suitable for the intravesical instillation of therapeutic agents, in vivo administration of retroviral vectors encoding IFN-γ may be explored for the treatment of bladder cancer.",
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Postoperative immuno-gene therapy of murine bladder tumor by in vivo administration of retroviruses expressing mouse interferon-γ. / Shiau, Ai-Li; Lin, Chih Yun; Tzai, Tzong Shin; Wu, Chao-Liang.

In: Cancer Gene Therapy, Vol. 8, No. 1, 01.01.2001, p. 73-81.

Research output: Contribution to journalArticle

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