Prc contributes to Escherichia coli evasion of classical complement-mediated serum killing

Chin Ya Wang, Shainn Wei Wang, Wen Chun Huang, Kwang Sik Kim, Nan Shan Chang, Ying Hsiang Wang, Meng Hsing Wu, Ching Hao Teng

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Escherichia coli is a common Gram-negative organism that causes bacteremia. Prc, a bacterial periplasmic protease, and its homologues are known to be involved in the pathogenesis of Gram-negative bacterial infections. The present study examined the role of Prc in E. coli bacteremia and characterized the ability of the prc mutant of the pathogenic E. coli strain RS218 to cause bacteremia and survive in human serum. The prc mutant of RS218 exhibited a decreased ability to cause a high level of bacteremia and was more sensitive to serum killing than strain RS218. This sensitivity was due to the mutant's decreased ability to avoid the activation of the antibody-dependent and -independent classical complement cascades as well as its decreased resistance to killing mediated by the membrane attack complex, the end product of complement system activation. The demonstration of Prc in the evasion of classical complement-mediated serum killing of pathogenic E. coli makes this factor a potential target for the development of therapeutic and preventive measures against E. coli bacteremia.

Original languageEnglish
Pages (from-to)3399-3409
Number of pages11
JournalInfection and Immunity
Volume80
Issue number10
DOIs
Publication statusPublished - 2012 Oct

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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