Preexisting antibody response against 2009 pandemic influenza H1N1 viruses in the Taiwanese population

Chia Yu Chi, Ching-Chuan Liu, Chia Chun Lin, Hsuan Chen Wang, Yung Tsung Cheng, Chung Ming Chang, Jen-Ren Wang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A novel pandemic influenza H1N1 (pH1N1) virus spread rapidly across the world in 2009. Due to the important role of antibody-mediated immunity in protection against influenza infection, we used an enzyme-linked immunosorbent assay-based microneutralization test to investigate cross-reactive neutralizing antibodies against the 2009 pH1N1 virus in 229 stored sera from donors born between 1917 and 2008 in Taiwan. The peak of cumulative geometric mean titers occurred in donors more than 90 years old and declined sharply with decreasing age. Sixteen of 27 subjects (59%) more than 80 years old had cross-reactive antibody titers of 160 or more against the 2009 pH1N1 virus, whereas none of the donors from age 9 to 49 had an antibody titer of 160 or more. Interestingly, 2 of 51 children (4%) from 6 months to 9 years old had an antibody titer of 40. We further tested the antibody responses in 9 of the 51 pediatric sera to three endemic seasonal influenza viruses isolated in 2006 and 2008 in Taiwan, and the results showed that only the 2 sera from children with antibody responses to the 2009 pH1N1 virus had high titers of neutralizing antibody against recent seasonal influenza virus strains. Our study shows the presence of some level of cross-reactive antibody in Taiwanese persons 50 years old or older, and the elderly subjects who may already have been exposed to the 1918 virus had high titers of neutralizing antibody to the 2009 pH1N1 virus. Our data also indicate that natural infection with the Taiwan 2006 and 2008 seasonal H1N1 viruses may induce a cross-reactive antibody response to the 2009 pH1N1 virus.

Original languageEnglish
Pages (from-to)1958-1962
Number of pages5
JournalClinical and Vaccine Immunology
Volume17
Issue number12
DOIs
Publication statusPublished - 2010 Dec 1

Fingerprint

H1N1 Subtype Influenza A Virus
Pandemics
Orthomyxoviridae
Viruses
Antibody Formation
Antibodies
Population
Neutralizing Antibodies
Taiwan
Tissue Donors
Serum
Infection
Immunosorbents
Pediatrics
Human Influenza
Immunity
Enzyme-Linked Immunosorbent Assay
Assays

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Chi, Chia Yu ; Liu, Ching-Chuan ; Lin, Chia Chun ; Wang, Hsuan Chen ; Cheng, Yung Tsung ; Chang, Chung Ming ; Wang, Jen-Ren. / Preexisting antibody response against 2009 pandemic influenza H1N1 viruses in the Taiwanese population. In: Clinical and Vaccine Immunology. 2010 ; Vol. 17, No. 12. pp. 1958-1962.
@article{5ef59055df8e4dd4aca40930b4136f71,
title = "Preexisting antibody response against 2009 pandemic influenza H1N1 viruses in the Taiwanese population",
abstract = "A novel pandemic influenza H1N1 (pH1N1) virus spread rapidly across the world in 2009. Due to the important role of antibody-mediated immunity in protection against influenza infection, we used an enzyme-linked immunosorbent assay-based microneutralization test to investigate cross-reactive neutralizing antibodies against the 2009 pH1N1 virus in 229 stored sera from donors born between 1917 and 2008 in Taiwan. The peak of cumulative geometric mean titers occurred in donors more than 90 years old and declined sharply with decreasing age. Sixteen of 27 subjects (59{\%}) more than 80 years old had cross-reactive antibody titers of 160 or more against the 2009 pH1N1 virus, whereas none of the donors from age 9 to 49 had an antibody titer of 160 or more. Interestingly, 2 of 51 children (4{\%}) from 6 months to 9 years old had an antibody titer of 40. We further tested the antibody responses in 9 of the 51 pediatric sera to three endemic seasonal influenza viruses isolated in 2006 and 2008 in Taiwan, and the results showed that only the 2 sera from children with antibody responses to the 2009 pH1N1 virus had high titers of neutralizing antibody against recent seasonal influenza virus strains. Our study shows the presence of some level of cross-reactive antibody in Taiwanese persons 50 years old or older, and the elderly subjects who may already have been exposed to the 1918 virus had high titers of neutralizing antibody to the 2009 pH1N1 virus. Our data also indicate that natural infection with the Taiwan 2006 and 2008 seasonal H1N1 viruses may induce a cross-reactive antibody response to the 2009 pH1N1 virus.",
author = "Chi, {Chia Yu} and Ching-Chuan Liu and Lin, {Chia Chun} and Wang, {Hsuan Chen} and Cheng, {Yung Tsung} and Chang, {Chung Ming} and Jen-Ren Wang",
year = "2010",
month = "12",
day = "1",
doi = "10.1128/CVI.00212-10",
language = "English",
volume = "17",
pages = "1958--1962",
journal = "Clinical and Vaccine Immunology",
issn = "1556-6811",
publisher = "American Society for Microbiology",
number = "12",

}

Preexisting antibody response against 2009 pandemic influenza H1N1 viruses in the Taiwanese population. / Chi, Chia Yu; Liu, Ching-Chuan; Lin, Chia Chun; Wang, Hsuan Chen; Cheng, Yung Tsung; Chang, Chung Ming; Wang, Jen-Ren.

In: Clinical and Vaccine Immunology, Vol. 17, No. 12, 01.12.2010, p. 1958-1962.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Preexisting antibody response against 2009 pandemic influenza H1N1 viruses in the Taiwanese population

AU - Chi, Chia Yu

AU - Liu, Ching-Chuan

AU - Lin, Chia Chun

AU - Wang, Hsuan Chen

AU - Cheng, Yung Tsung

AU - Chang, Chung Ming

AU - Wang, Jen-Ren

PY - 2010/12/1

Y1 - 2010/12/1

N2 - A novel pandemic influenza H1N1 (pH1N1) virus spread rapidly across the world in 2009. Due to the important role of antibody-mediated immunity in protection against influenza infection, we used an enzyme-linked immunosorbent assay-based microneutralization test to investigate cross-reactive neutralizing antibodies against the 2009 pH1N1 virus in 229 stored sera from donors born between 1917 and 2008 in Taiwan. The peak of cumulative geometric mean titers occurred in donors more than 90 years old and declined sharply with decreasing age. Sixteen of 27 subjects (59%) more than 80 years old had cross-reactive antibody titers of 160 or more against the 2009 pH1N1 virus, whereas none of the donors from age 9 to 49 had an antibody titer of 160 or more. Interestingly, 2 of 51 children (4%) from 6 months to 9 years old had an antibody titer of 40. We further tested the antibody responses in 9 of the 51 pediatric sera to three endemic seasonal influenza viruses isolated in 2006 and 2008 in Taiwan, and the results showed that only the 2 sera from children with antibody responses to the 2009 pH1N1 virus had high titers of neutralizing antibody against recent seasonal influenza virus strains. Our study shows the presence of some level of cross-reactive antibody in Taiwanese persons 50 years old or older, and the elderly subjects who may already have been exposed to the 1918 virus had high titers of neutralizing antibody to the 2009 pH1N1 virus. Our data also indicate that natural infection with the Taiwan 2006 and 2008 seasonal H1N1 viruses may induce a cross-reactive antibody response to the 2009 pH1N1 virus.

AB - A novel pandemic influenza H1N1 (pH1N1) virus spread rapidly across the world in 2009. Due to the important role of antibody-mediated immunity in protection against influenza infection, we used an enzyme-linked immunosorbent assay-based microneutralization test to investigate cross-reactive neutralizing antibodies against the 2009 pH1N1 virus in 229 stored sera from donors born between 1917 and 2008 in Taiwan. The peak of cumulative geometric mean titers occurred in donors more than 90 years old and declined sharply with decreasing age. Sixteen of 27 subjects (59%) more than 80 years old had cross-reactive antibody titers of 160 or more against the 2009 pH1N1 virus, whereas none of the donors from age 9 to 49 had an antibody titer of 160 or more. Interestingly, 2 of 51 children (4%) from 6 months to 9 years old had an antibody titer of 40. We further tested the antibody responses in 9 of the 51 pediatric sera to three endemic seasonal influenza viruses isolated in 2006 and 2008 in Taiwan, and the results showed that only the 2 sera from children with antibody responses to the 2009 pH1N1 virus had high titers of neutralizing antibody against recent seasonal influenza virus strains. Our study shows the presence of some level of cross-reactive antibody in Taiwanese persons 50 years old or older, and the elderly subjects who may already have been exposed to the 1918 virus had high titers of neutralizing antibody to the 2009 pH1N1 virus. Our data also indicate that natural infection with the Taiwan 2006 and 2008 seasonal H1N1 viruses may induce a cross-reactive antibody response to the 2009 pH1N1 virus.

UR - http://www.scopus.com/inward/record.url?scp=78649863631&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649863631&partnerID=8YFLogxK

U2 - 10.1128/CVI.00212-10

DO - 10.1128/CVI.00212-10

M3 - Article

VL - 17

SP - 1958

EP - 1962

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 12

ER -