Preparation, characterization and cytotoxicity of well-dispersed Fe 3O4 nanoparticles in aqueous solution and their potential as contrast agents

F. Y. Chane, C. H. Su, Y. S. Yang, Chen-Sheng Yeh, C. Y. Tsai, Chao-Liang Wu, M. T. Wu, Dar-Bin Shieh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Well-dispersed Fe3O4 nanoparticles were synthesized using Fe(II) and Fe(III) salt chemical coprecipitation with tetramethylammonium hydroxide (N(CH3)4OH) in an aqueous solution. TEM, X-ray diffraction (XRD), FT-IR, X-ray photoelectron spectrometer (XPS) and superconducting quantum interference measurement device (SQUID) measurements were employed to investigate the iron oxide properties. The resulting iron oxide particles were manipulated as small as 6 nm diameter in size. Based on FT-IR and X-ray photoelectron spectrometer results, it is suggested that the surfaces of the magnetite (Fe3O4) particles are covered with hydroxide (-OH) groups incorporated with (CH3)4N + through electrostatic interaction. The cytotoxicity test revealed that the magnetite particles exhibited in vitro biocompatibility with mouse fibroblasts, suggesting that they can be further explored for biomedical applications. NMR measurements revealed significantly reducing water proton relaxation times T1 and T2. The MR image of the nanoparticles in water and serum was investigated. Significant reduction of the background medium signal was achieved in the T2-weigted and the T2*-weighted sequence especially in the serum. Combined the advantage of MRI signal contrast, the versatile surface chemistry for bioconjugation and the homogenous nanometer size for better controlled biodistribution, these preliminary experiments demonstrated the potential of the as-synthesized magnetite material in functional molecular imaging for biomedical research and clinical diagnosis.

Original languageEnglish
Title of host publicationTransactions - 7th World Biomaterials Congress
Number of pages1
Publication statusPublished - 2004 Dec 1
EventTransactions - 7th World Biomaterials Congress - Sydney, Australia
Duration: 2004 May 172004 May 21

Publication series

NameTransactions - 7th World Biomaterials Congress

Other

OtherTransactions - 7th World Biomaterials Congress
CountryAustralia
CitySydney
Period04-05-1704-05-21

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Magnetite
Cytotoxicity
Photoelectrons
Nanoparticles
Iron oxides
Spectrometers
Molecular imaging
X rays
Fibroblasts
Coprecipitation
Coulomb interactions
Surface chemistry
Biocompatibility
Magnetic resonance imaging
Relaxation time
Water
Protons
Nuclear magnetic resonance
Salts
Transmission electron microscopy

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Chane, F. Y., Su, C. H., Yang, Y. S., Yeh, C-S., Tsai, C. Y., Wu, C-L., ... Shieh, D-B. (2004). Preparation, characterization and cytotoxicity of well-dispersed Fe 3O4 nanoparticles in aqueous solution and their potential as contrast agents. In Transactions - 7th World Biomaterials Congress (Transactions - 7th World Biomaterials Congress).
Chane, F. Y. ; Su, C. H. ; Yang, Y. S. ; Yeh, Chen-Sheng ; Tsai, C. Y. ; Wu, Chao-Liang ; Wu, M. T. ; Shieh, Dar-Bin. / Preparation, characterization and cytotoxicity of well-dispersed Fe 3O4 nanoparticles in aqueous solution and their potential as contrast agents. Transactions - 7th World Biomaterials Congress. 2004. (Transactions - 7th World Biomaterials Congress).
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abstract = "Well-dispersed Fe3O4 nanoparticles were synthesized using Fe(II) and Fe(III) salt chemical coprecipitation with tetramethylammonium hydroxide (N(CH3)4OH) in an aqueous solution. TEM, X-ray diffraction (XRD), FT-IR, X-ray photoelectron spectrometer (XPS) and superconducting quantum interference measurement device (SQUID) measurements were employed to investigate the iron oxide properties. The resulting iron oxide particles were manipulated as small as 6 nm diameter in size. Based on FT-IR and X-ray photoelectron spectrometer results, it is suggested that the surfaces of the magnetite (Fe3O4) particles are covered with hydroxide (-OH) groups incorporated with (CH3)4N + through electrostatic interaction. The cytotoxicity test revealed that the magnetite particles exhibited in vitro biocompatibility with mouse fibroblasts, suggesting that they can be further explored for biomedical applications. NMR measurements revealed significantly reducing water proton relaxation times T1 and T2. The MR image of the nanoparticles in water and serum was investigated. Significant reduction of the background medium signal was achieved in the T2-weigted and the T2*-weighted sequence especially in the serum. Combined the advantage of MRI signal contrast, the versatile surface chemistry for bioconjugation and the homogenous nanometer size for better controlled biodistribution, these preliminary experiments demonstrated the potential of the as-synthesized magnetite material in functional molecular imaging for biomedical research and clinical diagnosis.",
author = "Chane, {F. Y.} and Su, {C. H.} and Yang, {Y. S.} and Chen-Sheng Yeh and Tsai, {C. Y.} and Chao-Liang Wu and Wu, {M. T.} and Dar-Bin Shieh",
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Chane, FY, Su, CH, Yang, YS, Yeh, C-S, Tsai, CY, Wu, C-L, Wu, MT & Shieh, D-B 2004, Preparation, characterization and cytotoxicity of well-dispersed Fe 3O4 nanoparticles in aqueous solution and their potential as contrast agents. in Transactions - 7th World Biomaterials Congress. Transactions - 7th World Biomaterials Congress, Transactions - 7th World Biomaterials Congress, Sydney, Australia, 04-05-17.

Preparation, characterization and cytotoxicity of well-dispersed Fe 3O4 nanoparticles in aqueous solution and their potential as contrast agents. / Chane, F. Y.; Su, C. H.; Yang, Y. S.; Yeh, Chen-Sheng; Tsai, C. Y.; Wu, Chao-Liang; Wu, M. T.; Shieh, Dar-Bin.

Transactions - 7th World Biomaterials Congress. 2004. (Transactions - 7th World Biomaterials Congress).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Preparation, characterization and cytotoxicity of well-dispersed Fe 3O4 nanoparticles in aqueous solution and their potential as contrast agents

AU - Chane, F. Y.

AU - Su, C. H.

AU - Yang, Y. S.

AU - Yeh, Chen-Sheng

AU - Tsai, C. Y.

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AU - Shieh, Dar-Bin

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N2 - Well-dispersed Fe3O4 nanoparticles were synthesized using Fe(II) and Fe(III) salt chemical coprecipitation with tetramethylammonium hydroxide (N(CH3)4OH) in an aqueous solution. TEM, X-ray diffraction (XRD), FT-IR, X-ray photoelectron spectrometer (XPS) and superconducting quantum interference measurement device (SQUID) measurements were employed to investigate the iron oxide properties. The resulting iron oxide particles were manipulated as small as 6 nm diameter in size. Based on FT-IR and X-ray photoelectron spectrometer results, it is suggested that the surfaces of the magnetite (Fe3O4) particles are covered with hydroxide (-OH) groups incorporated with (CH3)4N + through electrostatic interaction. The cytotoxicity test revealed that the magnetite particles exhibited in vitro biocompatibility with mouse fibroblasts, suggesting that they can be further explored for biomedical applications. NMR measurements revealed significantly reducing water proton relaxation times T1 and T2. The MR image of the nanoparticles in water and serum was investigated. Significant reduction of the background medium signal was achieved in the T2-weigted and the T2*-weighted sequence especially in the serum. Combined the advantage of MRI signal contrast, the versatile surface chemistry for bioconjugation and the homogenous nanometer size for better controlled biodistribution, these preliminary experiments demonstrated the potential of the as-synthesized magnetite material in functional molecular imaging for biomedical research and clinical diagnosis.

AB - Well-dispersed Fe3O4 nanoparticles were synthesized using Fe(II) and Fe(III) salt chemical coprecipitation with tetramethylammonium hydroxide (N(CH3)4OH) in an aqueous solution. TEM, X-ray diffraction (XRD), FT-IR, X-ray photoelectron spectrometer (XPS) and superconducting quantum interference measurement device (SQUID) measurements were employed to investigate the iron oxide properties. The resulting iron oxide particles were manipulated as small as 6 nm diameter in size. Based on FT-IR and X-ray photoelectron spectrometer results, it is suggested that the surfaces of the magnetite (Fe3O4) particles are covered with hydroxide (-OH) groups incorporated with (CH3)4N + through electrostatic interaction. The cytotoxicity test revealed that the magnetite particles exhibited in vitro biocompatibility with mouse fibroblasts, suggesting that they can be further explored for biomedical applications. NMR measurements revealed significantly reducing water proton relaxation times T1 and T2. The MR image of the nanoparticles in water and serum was investigated. Significant reduction of the background medium signal was achieved in the T2-weigted and the T2*-weighted sequence especially in the serum. Combined the advantage of MRI signal contrast, the versatile surface chemistry for bioconjugation and the homogenous nanometer size for better controlled biodistribution, these preliminary experiments demonstrated the potential of the as-synthesized magnetite material in functional molecular imaging for biomedical research and clinical diagnosis.

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M3 - Conference contribution

AN - SCOPUS:13844254547

SN - 1877040193

SN - 9781877040191

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BT - Transactions - 7th World Biomaterials Congress

ER -

Chane FY, Su CH, Yang YS, Yeh C-S, Tsai CY, Wu C-L et al. Preparation, characterization and cytotoxicity of well-dispersed Fe 3O4 nanoparticles in aqueous solution and their potential as contrast agents. In Transactions - 7th World Biomaterials Congress. 2004. (Transactions - 7th World Biomaterials Congress).