Abstract
An electrochemical sensor based on an enzymatic poly[aniline-co-sodium N-(1-one-butyric acid) aniline]-zinc oxide (SPAnNa-ZnO) nanocomposite-modified gold electrode was developed for the determination of hydrogen peroxide (H 2O 2). The SPAnNa-ZnO nanocomposites dispersed stably in an aqueous solution because the small quantity of polyelectrolytic SPAnNa on the ZnO surface prevented nanocomposite aggregation. In addition, the electroactivity and electrochemical behavior of SPAnNa were enhanced in phosphate buffer solution at pH 7.0 by hybridization with spherical ZnO nanoparticles. These negatively charged SPAnNa-ZnO nanocomposites provided a favorable environment for the immobilization of horseradish peroxidase (HRP) by electrostatic interactions. The load quantity of immobilized HRP was approximately 15 wt% on the SPAnNa-ZnO nanocomposite. This non-destructive immobilization maintained the activity of HRP, thereby enabling effective H 2O 2 detection. The sensor exhibited a linear detection range from 10 μM to 2 mM with a relatively high sensitivity of 519.8 μA mM -1 cm -2 at an applied potential of -0.4 V.
| Original language | English |
|---|---|
| Pages (from-to) | 13252-13259 |
| Number of pages | 8 |
| Journal | Journal of Materials Chemistry |
| Volume | 22 |
| Issue number | 26 |
| DOIs | |
| Publication status | Published - 2012 Jul 14 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Materials Chemistry
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Preparation of water-dispersible poly[aniline-co-sodium N-(1-one-butyric acid) aniline]-zinc oxide nanocomposite for utilization in an electrochemical sensor. / Chen, Hsiao Chien; Hua, Mu Yi; Liu, Yin Chih; Yang, Hung Wei; Tsai, Rung Ywan.
In: Journal of Materials Chemistry, Vol. 22, No. 26, 14.07.2012, p. 13252-13259.Research output: Contribution to journal › Article
TY - JOUR
T1 - Preparation of water-dispersible poly[aniline-co-sodium N-(1-one-butyric acid) aniline]-zinc oxide nanocomposite for utilization in an electrochemical sensor
AU - Chen, Hsiao Chien
AU - Hua, Mu Yi
AU - Liu, Yin Chih
AU - Yang, Hung Wei
AU - Tsai, Rung Ywan
PY - 2012/7/14
Y1 - 2012/7/14
N2 - An electrochemical sensor based on an enzymatic poly[aniline-co-sodium N-(1-one-butyric acid) aniline]-zinc oxide (SPAnNa-ZnO) nanocomposite-modified gold electrode was developed for the determination of hydrogen peroxide (H 2O 2). The SPAnNa-ZnO nanocomposites dispersed stably in an aqueous solution because the small quantity of polyelectrolytic SPAnNa on the ZnO surface prevented nanocomposite aggregation. In addition, the electroactivity and electrochemical behavior of SPAnNa were enhanced in phosphate buffer solution at pH 7.0 by hybridization with spherical ZnO nanoparticles. These negatively charged SPAnNa-ZnO nanocomposites provided a favorable environment for the immobilization of horseradish peroxidase (HRP) by electrostatic interactions. The load quantity of immobilized HRP was approximately 15 wt% on the SPAnNa-ZnO nanocomposite. This non-destructive immobilization maintained the activity of HRP, thereby enabling effective H 2O 2 detection. The sensor exhibited a linear detection range from 10 μM to 2 mM with a relatively high sensitivity of 519.8 μA mM -1 cm -2 at an applied potential of -0.4 V.
AB - An electrochemical sensor based on an enzymatic poly[aniline-co-sodium N-(1-one-butyric acid) aniline]-zinc oxide (SPAnNa-ZnO) nanocomposite-modified gold electrode was developed for the determination of hydrogen peroxide (H 2O 2). The SPAnNa-ZnO nanocomposites dispersed stably in an aqueous solution because the small quantity of polyelectrolytic SPAnNa on the ZnO surface prevented nanocomposite aggregation. In addition, the electroactivity and electrochemical behavior of SPAnNa were enhanced in phosphate buffer solution at pH 7.0 by hybridization with spherical ZnO nanoparticles. These negatively charged SPAnNa-ZnO nanocomposites provided a favorable environment for the immobilization of horseradish peroxidase (HRP) by electrostatic interactions. The load quantity of immobilized HRP was approximately 15 wt% on the SPAnNa-ZnO nanocomposite. This non-destructive immobilization maintained the activity of HRP, thereby enabling effective H 2O 2 detection. The sensor exhibited a linear detection range from 10 μM to 2 mM with a relatively high sensitivity of 519.8 μA mM -1 cm -2 at an applied potential of -0.4 V.
UR - http://www.scopus.com/inward/record.url?scp=84862584272&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84862584272&partnerID=8YFLogxK
U2 - 10.1039/c2jm30775h
DO - 10.1039/c2jm30775h
M3 - Article
AN - SCOPUS:84862584272
VL - 22
SP - 13252
EP - 13259
JO - Journal of Materials Chemistry
JF - Journal of Materials Chemistry
SN - 0959-9428
IS - 26
ER -