Presence of DAZL transcript and protein in mature human spermatozoa

YungMing Lin, Chaio Wei Chen, Hsiao-Fang Sun, Shaw-Jenq Tsai, Johnny Shinn Nan Lin, Pao-Lin Kuo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objective: To identify the DAZL transcript and protein location in human spermatozoa. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): A fertile volunteer. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunostaining for DAZL. Main Outcome Measure(s): Expression of DAZL in human spermatozoa. Result(s): The DAZL-specific primers yield a 128 bp product in ejaculate. A protein of approximately 33.5 kDa was detected by Western blot analysis. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa and weak staining in the principal piece. A speckled-type distribution was found in the head region. Conclusion(s): The DAZL transcript and protein are present in human spermatozoa. The roles of DAZL protein in sperm motility and in the sperm-oocyte interaction await further investigation.

Original languageEnglish
Pages (from-to)626-629
Number of pages4
JournalFertility and Sterility
Volume77
Issue number3
DOIs
Publication statusPublished - 2002 Mar 9

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Spermatozoa
Staining and Labeling
Proteins
Western Blotting
Sperm-Ovum Interactions
Sperm Motility
Reverse Transcription
Fluorescent Antibody Technique
Volunteers
Head
Outcome Assessment (Health Care)
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

Cite this

Lin, YungMing ; Chen, Chaio Wei ; Sun, Hsiao-Fang ; Tsai, Shaw-Jenq ; Lin, Johnny Shinn Nan ; Kuo, Pao-Lin. / Presence of DAZL transcript and protein in mature human spermatozoa. In: Fertility and Sterility. 2002 ; Vol. 77, No. 3. pp. 626-629.
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abstract = "Objective: To identify the DAZL transcript and protein location in human spermatozoa. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): A fertile volunteer. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunostaining for DAZL. Main Outcome Measure(s): Expression of DAZL in human spermatozoa. Result(s): The DAZL-specific primers yield a 128 bp product in ejaculate. A protein of approximately 33.5 kDa was detected by Western blot analysis. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa and weak staining in the principal piece. A speckled-type distribution was found in the head region. Conclusion(s): The DAZL transcript and protein are present in human spermatozoa. The roles of DAZL protein in sperm motility and in the sperm-oocyte interaction await further investigation.",
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Presence of DAZL transcript and protein in mature human spermatozoa. / Lin, YungMing; Chen, Chaio Wei; Sun, Hsiao-Fang; Tsai, Shaw-Jenq; Lin, Johnny Shinn Nan; Kuo, Pao-Lin.

In: Fertility and Sterility, Vol. 77, No. 3, 09.03.2002, p. 626-629.

Research output: Contribution to journalArticle

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AU - Chen, Chaio Wei

AU - Sun, Hsiao-Fang

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AU - Lin, Johnny Shinn Nan

AU - Kuo, Pao-Lin

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