Presence of DAZL transcript and protein in mature human spermatozoa

Yung Ming Lin, Chaio Wei Chen, H. Sunny Sun, Shaw Jeng Tsai, Johnny Shinn Nan Lin, Pao Lin Kuo

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Objective: To identify the DAZL transcript and protein location in human spermatozoa. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): A fertile volunteer. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunostaining for DAZL. Main Outcome Measure(s): Expression of DAZL in human spermatozoa. Result(s): The DAZL-specific primers yield a 128 bp product in ejaculate. A protein of approximately 33.5 kDa was detected by Western blot analysis. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa and weak staining in the principal piece. A speckled-type distribution was found in the head region. Conclusion(s): The DAZL transcript and protein are present in human spermatozoa. The roles of DAZL protein in sperm motility and in the sperm-oocyte interaction await further investigation.

Original languageEnglish
Pages (from-to)626-629
Number of pages4
JournalFertility and Sterility
Volume77
Issue number3
DOIs
Publication statusPublished - 2002

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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