TY - JOUR
T1 - Presence of human herpesvirus-like DNA sequence in oral Kaposi's sarcoma A preliminary PCR study
AU - Jin, Ying Tai
AU - Tsai, Sen Tien
AU - Yan, Jing Jou
AU - Chen, Fen Fen
AU - Lee, Wen Ying
AU - Li, Wing Yin
AU - Chiang, Hung
AU - Su, Ih Jen
N1 - Funding Information:
Pathogens or factors suspected to be associated with Kaposi's sarcoma (KS) include human herpes virus type 6, cytomegalovirus, human immunodeficiency virus, mycoplasma penetrans, 1-3 sex hormone, 4 and nitrite inhalants. 5 However, solid evidence of etiologic association among any of these agents with acquired immunodeficiency syndrome (AIDS) or non-AIDS KS has not been established yet. Recently, a new herpesvirus-like DNA sequence was consistently identified by representative difference analysis method in AIDS KS tumor tissues. 6 The This study is partly supported by the National Science Council Grant (NSC85-2331-B-006-025). aAssociate Professor, Department of Pathology, National Cheng Knng University. bLecturer, Department of Otolaryngology,N ational Cheng Kung University. cChief Resident, Department of Pathology, National Cheng Kung University Hospital. dPathologist, Department of Pathology, National Cheng Kung University Hospital. ePathologist, Department of Pathology, National Cheng Kung University Hospital. fPathologist, Department of Pathology, Veterans General Hospital-Taipei. gProfessor and Chairman, Department of Pathology, Veterans General Hospital-Taipei. hprofessor and Chairman, Department of Pathology, National Cheng Kung University. Received for publication July 7, 1995; returned for revision Aug. 5, 19951 accepted for publication Nov. 6, 1995. Copyright 9 1996 by Mosby-Year B~ok, Inc. 1079-2104/96/$5.00 + 0 7/14/70661 emerging evidence suggests that this is an entirely new and unique herpesvirus that contains 270 kb pairs, making it the largest known herpesvirus, even though the virus has not been observed by electron microscopy\] Further studies revealed that this viral sequence can be identified by polymerase chain reaction (PCR) not only in AIDS KS but also in non-AIDS KS .8 The corresponding control skin and other nearby tissue from those KS patients were negative for this sequence. This DNA sequence has been consistently absent in normal control tissue and other types of human malignancies. 6-1l Because this new herpesvirus-like DNA sequence is most likely related to KS in AIDS and non-AIDS patients and the majority of patients with AIDS who develop KS have involvement of the oral cavity (71%),1a analysis of oral KS and other oral neoplasms was indicated. Therefore to verify the specificity of this herpesvirus-like DNA sequence in oral KS, we examined various types of oral neoplasms and three KS lesions from two patients with AIDS for the presence of this DNA sequence using the published KS 330-233 primers by PCR. 6
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996
Y1 - 1996
N2 - DNA sequences closely related to herpesvirus-like sequences have been found in AIDS-associated Kaposi's sarcoma. Subsequent studies indicate that these DNA sequences may be consistently found in AIDS Kaposi's sarcoma and non-AIDS Kaposi's sarcoma from different geographic regions. Oral Kaposi's sarcoma is rare and the specificity of this viral DNA in oral Kaposi's sarcoma and in other oral neoplasms has not been examined. A total of 77 oral neoplasms, including 3 Kaposi's sarcoma specimens from 2 patients with AIDS, were analyzed by polymerase chain reaction for the presence of this herpesvirus-like DNA sequence with the 330-233 primers. All three specimens of oral AIDS-Kaposi's sarcoma from two patients were positive for this DNA sequence. All of the other intraoral lesions including hemangioma, pyogenic granuloma, and various types of epithelial neoplasms, however, had negative reactions Our results further confirm that oral Kaposi's sarcoma appears to be associated with this herpesvirus-like DNA sequence.
AB - DNA sequences closely related to herpesvirus-like sequences have been found in AIDS-associated Kaposi's sarcoma. Subsequent studies indicate that these DNA sequences may be consistently found in AIDS Kaposi's sarcoma and non-AIDS Kaposi's sarcoma from different geographic regions. Oral Kaposi's sarcoma is rare and the specificity of this viral DNA in oral Kaposi's sarcoma and in other oral neoplasms has not been examined. A total of 77 oral neoplasms, including 3 Kaposi's sarcoma specimens from 2 patients with AIDS, were analyzed by polymerase chain reaction for the presence of this herpesvirus-like DNA sequence with the 330-233 primers. All three specimens of oral AIDS-Kaposi's sarcoma from two patients were positive for this DNA sequence. All of the other intraoral lesions including hemangioma, pyogenic granuloma, and various types of epithelial neoplasms, however, had negative reactions Our results further confirm that oral Kaposi's sarcoma appears to be associated with this herpesvirus-like DNA sequence.
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U2 - 10.1016/S1079-2104(96)80020-5
DO - 10.1016/S1079-2104(96)80020-5
M3 - Article
C2 - 8705590
AN - SCOPUS:0030120524
SN - 1079-2104
VL - 81
SP - 442
EP - 444
JO - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
JF - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
IS - 4
ER -
