Prevalence of Qnr determinants among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae in a Taiwanese Hospital, 1999-2005

Jiunn Jong Wu, Wen Chien Ko, Hsiu Mei Wu, Jing Jou Yan

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Abstract

Objectives: To determine the prevalence and characteristics of bloodstream isolates of Escherichia coli and Klebsiella pneumoniae with qnr genes in a Taiwanese hospital. Methods: A total of 2035 E. coli and 1147 K. pneumoniae isolates collected between 1999 and 2005 were screened for qnrA, qnrB and qnrS by PCR and colony hybridization. β-Lactamase genes, genetic relatedness and transferability were examined by PCR, PFGE and conjugation, respectively. Results The prevalence of qnr genes was 7.8% and 0.6% for K. pneumoniae and E. coli, respectively. The prevalence rates of qnrB2, qnrB4 and qnrS1 genes for K. pneumoniae were 2.3%, 3.6% and 2.8%, respectively, and for E. coli were 0.2%, 0% and 0.4%, respectively. The prevalence of qnrB4 in K. pneumoniae increased remarkably from 0% to 7.6% over the 7 study years. qnrA was not detected. Overall, the SHV-5-related, CTX-M-14, CTX-M-3, CMY-2, DHA-1 and IMP-8 β-lactamases were detected alone or in combination in 82.0% of qnr-positive K. pneumoniae isolates and 41.7% of qnr-positive E. coli isolates. Notably, all qnrB4 -positive isolates possessed the DHA-1 enzyme, and the majority of the qnrB2 -positive isolates (E. coli, 100%; K. pneumoniae, 80.8%) produced SHV-5-related β-lactamases. Genetic diversity was demonstrated by PFGE. Conjugation experiments revealed co-transfer of blaSHV-12, blaDHA-1 and blaSHV-5 with qnrB2, qnrB4 and qnrS1, respectively. Conclusions: qnr genes remained rare in E. coli but appeared to be increasing in K. pneumoniae in our hospital. Horizontal transfer may play a major role in the intra-hospital spread of qnr.

Original languageEnglish
Pages (from-to)1234-1239
Number of pages6
JournalJournal of Antimicrobial Chemotherapy
Volume61
Issue number6
DOIs
Publication statusPublished - 2008 Jun 1

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Klebsiella pneumoniae
Escherichia coli
Genes
Polymerase Chain Reaction
Enzymes

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Microbiology (medical)
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

@article{0298319e5dda4cdd98cf7cfbd46efbd3,
title = "Prevalence of Qnr determinants among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae in a Taiwanese Hospital, 1999-2005",
abstract = "Objectives: To determine the prevalence and characteristics of bloodstream isolates of Escherichia coli and Klebsiella pneumoniae with qnr genes in a Taiwanese hospital. Methods: A total of 2035 E. coli and 1147 K. pneumoniae isolates collected between 1999 and 2005 were screened for qnrA, qnrB and qnrS by PCR and colony hybridization. β-Lactamase genes, genetic relatedness and transferability were examined by PCR, PFGE and conjugation, respectively. Results The prevalence of qnr genes was 7.8{\%} and 0.6{\%} for K. pneumoniae and E. coli, respectively. The prevalence rates of qnrB2, qnrB4 and qnrS1 genes for K. pneumoniae were 2.3{\%}, 3.6{\%} and 2.8{\%}, respectively, and for E. coli were 0.2{\%}, 0{\%} and 0.4{\%}, respectively. The prevalence of qnrB4 in K. pneumoniae increased remarkably from 0{\%} to 7.6{\%} over the 7 study years. qnrA was not detected. Overall, the SHV-5-related, CTX-M-14, CTX-M-3, CMY-2, DHA-1 and IMP-8 β-lactamases were detected alone or in combination in 82.0{\%} of qnr-positive K. pneumoniae isolates and 41.7{\%} of qnr-positive E. coli isolates. Notably, all qnrB4 -positive isolates possessed the DHA-1 enzyme, and the majority of the qnrB2 -positive isolates (E. coli, 100{\%}; K. pneumoniae, 80.8{\%}) produced SHV-5-related β-lactamases. Genetic diversity was demonstrated by PFGE. Conjugation experiments revealed co-transfer of blaSHV-12, blaDHA-1 and blaSHV-5 with qnrB2, qnrB4 and qnrS1, respectively. Conclusions: qnr genes remained rare in E. coli but appeared to be increasing in K. pneumoniae in our hospital. Horizontal transfer may play a major role in the intra-hospital spread of qnr.",
author = "Wu, {Jiunn Jong} and Ko, {Wen Chien} and Wu, {Hsiu Mei} and Yan, {Jing Jou}",
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Prevalence of Qnr determinants among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae in a Taiwanese Hospital, 1999-2005. / Wu, Jiunn Jong; Ko, Wen Chien; Wu, Hsiu Mei; Yan, Jing Jou.

In: Journal of Antimicrobial Chemotherapy, Vol. 61, No. 6, 01.06.2008, p. 1234-1239.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prevalence of Qnr determinants among bloodstream isolates of Escherichia coli and Klebsiella pneumoniae in a Taiwanese Hospital, 1999-2005

AU - Wu, Jiunn Jong

AU - Ko, Wen Chien

AU - Wu, Hsiu Mei

AU - Yan, Jing Jou

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Objectives: To determine the prevalence and characteristics of bloodstream isolates of Escherichia coli and Klebsiella pneumoniae with qnr genes in a Taiwanese hospital. Methods: A total of 2035 E. coli and 1147 K. pneumoniae isolates collected between 1999 and 2005 were screened for qnrA, qnrB and qnrS by PCR and colony hybridization. β-Lactamase genes, genetic relatedness and transferability were examined by PCR, PFGE and conjugation, respectively. Results The prevalence of qnr genes was 7.8% and 0.6% for K. pneumoniae and E. coli, respectively. The prevalence rates of qnrB2, qnrB4 and qnrS1 genes for K. pneumoniae were 2.3%, 3.6% and 2.8%, respectively, and for E. coli were 0.2%, 0% and 0.4%, respectively. The prevalence of qnrB4 in K. pneumoniae increased remarkably from 0% to 7.6% over the 7 study years. qnrA was not detected. Overall, the SHV-5-related, CTX-M-14, CTX-M-3, CMY-2, DHA-1 and IMP-8 β-lactamases were detected alone or in combination in 82.0% of qnr-positive K. pneumoniae isolates and 41.7% of qnr-positive E. coli isolates. Notably, all qnrB4 -positive isolates possessed the DHA-1 enzyme, and the majority of the qnrB2 -positive isolates (E. coli, 100%; K. pneumoniae, 80.8%) produced SHV-5-related β-lactamases. Genetic diversity was demonstrated by PFGE. Conjugation experiments revealed co-transfer of blaSHV-12, blaDHA-1 and blaSHV-5 with qnrB2, qnrB4 and qnrS1, respectively. Conclusions: qnr genes remained rare in E. coli but appeared to be increasing in K. pneumoniae in our hospital. Horizontal transfer may play a major role in the intra-hospital spread of qnr.

AB - Objectives: To determine the prevalence and characteristics of bloodstream isolates of Escherichia coli and Klebsiella pneumoniae with qnr genes in a Taiwanese hospital. Methods: A total of 2035 E. coli and 1147 K. pneumoniae isolates collected between 1999 and 2005 were screened for qnrA, qnrB and qnrS by PCR and colony hybridization. β-Lactamase genes, genetic relatedness and transferability were examined by PCR, PFGE and conjugation, respectively. Results The prevalence of qnr genes was 7.8% and 0.6% for K. pneumoniae and E. coli, respectively. The prevalence rates of qnrB2, qnrB4 and qnrS1 genes for K. pneumoniae were 2.3%, 3.6% and 2.8%, respectively, and for E. coli were 0.2%, 0% and 0.4%, respectively. The prevalence of qnrB4 in K. pneumoniae increased remarkably from 0% to 7.6% over the 7 study years. qnrA was not detected. Overall, the SHV-5-related, CTX-M-14, CTX-M-3, CMY-2, DHA-1 and IMP-8 β-lactamases were detected alone or in combination in 82.0% of qnr-positive K. pneumoniae isolates and 41.7% of qnr-positive E. coli isolates. Notably, all qnrB4 -positive isolates possessed the DHA-1 enzyme, and the majority of the qnrB2 -positive isolates (E. coli, 100%; K. pneumoniae, 80.8%) produced SHV-5-related β-lactamases. Genetic diversity was demonstrated by PFGE. Conjugation experiments revealed co-transfer of blaSHV-12, blaDHA-1 and blaSHV-5 with qnrB2, qnrB4 and qnrS1, respectively. Conclusions: qnr genes remained rare in E. coli but appeared to be increasing in K. pneumoniae in our hospital. Horizontal transfer may play a major role in the intra-hospital spread of qnr.

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