Primary Cilium-Regulated EG-VEGF Signaling Facilitates Trophoblast Invasion

Chia-Yih Wang, Hui Ling Tsai, Jhih Siang Syu, Ting Yu Chen, Mei-Tsz Su

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Trophoblast invasion is an important event in embryo implantation and placental development. During these processes, endocrine gland-derived vascular endothelial growth factor (EG-VEGF) is the key regulator mediating the crosstalk at the feto-maternal interface. The primary cilium is a cellular antenna receiving environmental signals and is crucial for proper development. However, little is known regarding the role of the primary cilium in early human pregnancy. Here, we demonstrate that EG-VEGF regulates trophoblast cell invasion via primary cilia. We found that EG-VEGF activated ERK1/2 signaling and subsequent upregulation of MMP2 and MMP9, thereby facilitating cell invasion in human trophoblast HTR-8/SVneo cells. Inhibition of ERK1/2 alleviated the expression of MMPs and trophoblast cell invasion after EG-VEGF treatment. In addition, primary cilia were observed in all the trophoblast cell lines tested and, more importantly, in human first-trimester placental tissue. The receptor of EG-VEGF, PROKR1, was detected in primary cilia. Depletion of IFT88, the intraflagellar transporter required for ciliogenesis, inhibited primary cilium growth, thereby ameliorating ERK1/2 activation, MMP upregulation, and trophoblast cell invasion promoted by EG-VEGF. These findings demonstrate a novel function of primary cilia in controlling EG-VEGF-regulated trophoblast invasion and reveal the underlying molecular mechanism. J. Cell. Physiol. 232: 1467–1477, 2017.

Original languageEnglish
Pages (from-to)1467-1477
Number of pages11
JournalJournal of Cellular Physiology
Volume232
Issue number6
DOIs
Publication statusPublished - 2017 Jun 1

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Endocrine-Gland-Derived Vascular Endothelial Growth Factor
Cilia
Trophoblasts
Matrix Metalloproteinases
Up-Regulation
Placentation
Receiving antennas
First Pregnancy Trimester
Crosstalk
Embryonic Development
Chemical activation
Cells
Mothers
Tissue
Cell Line
Pregnancy

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Wang, Chia-Yih ; Tsai, Hui Ling ; Syu, Jhih Siang ; Chen, Ting Yu ; Su, Mei-Tsz. / Primary Cilium-Regulated EG-VEGF Signaling Facilitates Trophoblast Invasion. In: Journal of Cellular Physiology. 2017 ; Vol. 232, No. 6. pp. 1467-1477.
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Primary Cilium-Regulated EG-VEGF Signaling Facilitates Trophoblast Invasion. / Wang, Chia-Yih; Tsai, Hui Ling; Syu, Jhih Siang; Chen, Ting Yu; Su, Mei-Tsz.

In: Journal of Cellular Physiology, Vol. 232, No. 6, 01.06.2017, p. 1467-1477.

Research output: Contribution to journalArticle

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