Primary culture of rat adrenocortical cells and assays of steroidogenic functions

Yung Chia Chen, Bu-Miin Huang

Research output: Contribution to journalArticle

Abstract

The hormone which the adrenal cortex secretes is vital for animals against stress and diseases. The method described here is the procedure of primary cultured rat adrenal cells and related functional assays (immunofluorescence staining of lipid droplet surface protein, as well as corticosterone analysis). Unlike an in vivo model, the variation of interexperiments in adrenal monolayer cultures is less and the experimental condition is easy to control. Besides, the source of rats is also more stable than other animals, like bovine ones. There are also several human adrenal cell lines (NCI-H295, NCI-H295R, SW13, etc.) that can be used in adrenal studies. However, the steroid production of these lines will still be influenced by numerous factors, which include serum lot number, passage number, mutant/loss of distinct genes, etc. Except for lacking 17a-hydroxylase, the primary culture of rat adrenocortical cells is a better and more convenient technique for studying adrenal physiology. In summary, primary rat adrenal cultures could be a good in vitro platform for researchers to investigate the mechanisms of the reagent of interest in the adrenal gland system.

Original languageEnglish
Article numbere59016
JournalJournal of Visualized Experiments
Volume2019
Issue number145
DOIs
Publication statusPublished - 2019 Mar 1

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Rats
Assays
Animals
Hormones
Physiology
Adrenal Glands
Corticosterone
Mixed Function Oxygenases
Lipids
Fluorescent Antibody Technique
Monolayers
Adrenal Cortex Hormones
Membrane Proteins
Genes
Steroids
Cells
Research Personnel
Staining and Labeling
Proteins
Cell Line

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Chemical Engineering(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

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Primary culture of rat adrenocortical cells and assays of steroidogenic functions. / Chen, Yung Chia; Huang, Bu-Miin.

In: Journal of Visualized Experiments, Vol. 2019, No. 145, e59016, 01.03.2019.

Research output: Contribution to journalArticle

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