TY - JOUR
T1 - Producing 2,3-butanediol from agricultural waste using an indigenous Klebsiella sp. Zmd30 strain
AU - Wong, Chiao Ling
AU - Huang, Chien Chang
AU - Lu, Wei Bin
AU - Chen, Wen Ming
AU - Chang, Jo Shu
N1 - Funding Information:
The authors gratefully acknowledge financial support from Taiwan's National Science Council under grant numbers 99-2221-E-006-137-MY3 , 98-2221-E-006-240-MY3 , 100-3113-E-006-016 , and 101-3113-E-006-015 . The support received from the Top University grant (also known as “5-year-50-billion” grant) of National Cheng Kung University is also appreciated.
Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2012/12/15
Y1 - 2012/12/15
N2 - This study aimed to develop a low cost and high efficiency process for 2,3-butanediol (2,3-BDO) production by using Klebsiella sp. Zmd30 isolated from a hydrogen-producing anaerobic sludge. First, the effects of glucose concentration and nitrogen source on 2,3-BDO production with the Zmd30 strain were examined. The results show that the productivity and concentration of 2,3-BDO reached the highest values of 0.92g/l/h and 58g/l, respectively, when the glucose concentration was in the range of 160-180g/l. Urea was a better nitrogen source when considering both the 2,3-BDO production performance and the cost. Next, medium composition for the growth of the Zmd30 strain was optimized using the response surface methodology (RSM) to enhance 2,3-BDO production. Based on the RSM analysis, the optimal composition of glucose, urea and MnCl2·4H2O, H3BO3 and CoCl2·6H2O was 160, 2.28, 7.01×10-4, 1.49×10-4 and 5.70×10-3g/l, respectively. With this optimal medium, the 2,3-BDO productivity was improved to 1.15g/l/h. To reduce the production cost, bagasse and rice straw were used as feedstock for 2,3-BDO production. It was found that using hydrolyzed rice straw resulted in better productivity than using hydrolyzed bagasse, as the highest productivity (2.08g/l/h) and yield (62%) were obtained at an initial rice straw concentration of 150g/l.
AB - This study aimed to develop a low cost and high efficiency process for 2,3-butanediol (2,3-BDO) production by using Klebsiella sp. Zmd30 isolated from a hydrogen-producing anaerobic sludge. First, the effects of glucose concentration and nitrogen source on 2,3-BDO production with the Zmd30 strain were examined. The results show that the productivity and concentration of 2,3-BDO reached the highest values of 0.92g/l/h and 58g/l, respectively, when the glucose concentration was in the range of 160-180g/l. Urea was a better nitrogen source when considering both the 2,3-BDO production performance and the cost. Next, medium composition for the growth of the Zmd30 strain was optimized using the response surface methodology (RSM) to enhance 2,3-BDO production. Based on the RSM analysis, the optimal composition of glucose, urea and MnCl2·4H2O, H3BO3 and CoCl2·6H2O was 160, 2.28, 7.01×10-4, 1.49×10-4 and 5.70×10-3g/l, respectively. With this optimal medium, the 2,3-BDO productivity was improved to 1.15g/l/h. To reduce the production cost, bagasse and rice straw were used as feedstock for 2,3-BDO production. It was found that using hydrolyzed rice straw resulted in better productivity than using hydrolyzed bagasse, as the highest productivity (2.08g/l/h) and yield (62%) were obtained at an initial rice straw concentration of 150g/l.
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U2 - 10.1016/j.bej.2012.08.006
DO - 10.1016/j.bej.2012.08.006
M3 - Article
AN - SCOPUS:84865830125
VL - 69
SP - 32
EP - 40
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
SN - 1369-703X
ER -