TY - JOUR
T1 - Production and characterization of exopolysaccharides and antioxidant from Paenibacillus sp. TKU023
AU - Wang, Chuan Lu
AU - Huang, Tzu Huang
AU - Liang, Tzu Wen
AU - Fang, Chun Yong
AU - Wang, San Lang
N1 - Funding Information:
This work was supported in part by a grant of the National Science Council, Taiwan (NSC99-2313-B-032-001-MY3).
PY - 2011/10
Y1 - 2011/10
N2 - Using squid pen powder (SPP) as the sole C/N source, Paenibacillus sp. TKU023 produced exopolysaccharides (EPS) and antioxidant. With medium containing 1.5% SPP, 0.1% K2HPO4, and 0.05% MgSO4·7H2O, pH 7.23, the culture was incubated at 37°C in liquid (50mL/250mL) for five days. The resultant culture supernatant had higher EPS productivity (4.55g/L). The crude EPS were isolated by centrifugation, methanol precipitation and deproteinization. The characterization of the EPS demonstrated that it was mainly composed of glucose and maltose. In addition, the culture supernatant incubated for four days by using baffled base flask showed the strongest antioxidant activities and the highest total phenolic content, but maximum EPS production was found at the fifth day by using flat base flask. The production of two invaluable environmental-friendly biomaterials (EPS and antioxidant) is unprecedented. Besides, the use of SPP (waste) is green that made the whole process more valuable and attractive.
AB - Using squid pen powder (SPP) as the sole C/N source, Paenibacillus sp. TKU023 produced exopolysaccharides (EPS) and antioxidant. With medium containing 1.5% SPP, 0.1% K2HPO4, and 0.05% MgSO4·7H2O, pH 7.23, the culture was incubated at 37°C in liquid (50mL/250mL) for five days. The resultant culture supernatant had higher EPS productivity (4.55g/L). The crude EPS were isolated by centrifugation, methanol precipitation and deproteinization. The characterization of the EPS demonstrated that it was mainly composed of glucose and maltose. In addition, the culture supernatant incubated for four days by using baffled base flask showed the strongest antioxidant activities and the highest total phenolic content, but maximum EPS production was found at the fifth day by using flat base flask. The production of two invaluable environmental-friendly biomaterials (EPS and antioxidant) is unprecedented. Besides, the use of SPP (waste) is green that made the whole process more valuable and attractive.
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U2 - 10.1016/j.nbt.2011.03.003
DO - 10.1016/j.nbt.2011.03.003
M3 - Article
C2 - 21402186
AN - SCOPUS:80052962879
SN - 1871-6784
VL - 28
SP - 559
EP - 565
JO - New Biotechnology
JF - New Biotechnology
IS - 6
ER -