TY - JOUR
T1 - Production, isolation and characterization of C-phycocyanin from a new halo-tolerant Cyanobacterium aponinum using seawater
AU - Lin, Jia Yi
AU - Ng, I. Son
N1 - Funding Information:
The authors are grateful to the financial support for this study provided by the Ministry of Science and Technology ( MOST 108-2221-E-006-004-MY3 and MOST 110-2221-E-006-030-MY3 ) in Taiwan.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/12
Y1 - 2021/12
N2 - A halo-tolerant Cyanobacterium aponinum PCC 10605 was applied for the first time to produce high-level C-phycocyanin (C-PC). Combined with chemical extraction with sodium phosphate buffer and physical treatment using high pressure homogenization, a higher titer of C-PC was achieved. The culture conditions were optimized by mixing nitrate and ammonia ions, 2% carbon dioxide, and conditional light intensity. Thus, strain PCC10605 produced the highest titer C-PC of 0.652 g/g-DCW in the N1A2 medium with 10% light intensity and 16:8 light-period on day 7. PCC10605 accumulated 0.51 g-CPC/g-DCW at 20 g/L NaCl, while it grew normally in seawater with 30 g/L salinity, thus confirmed that PCC10605 was halo-tolerant strain. Besides, PCC10605 survived in 0.12 g/L phosphate medium that has never been reported. Finally, the purified C-PC exhibited DPPH, superoxide scavenging activity and antibacterial activity, which displayed 87.6%, and 18.7% removal of free radical, and 1.98 cm of inhibition zone for Escherichia coli.
AB - A halo-tolerant Cyanobacterium aponinum PCC 10605 was applied for the first time to produce high-level C-phycocyanin (C-PC). Combined with chemical extraction with sodium phosphate buffer and physical treatment using high pressure homogenization, a higher titer of C-PC was achieved. The culture conditions were optimized by mixing nitrate and ammonia ions, 2% carbon dioxide, and conditional light intensity. Thus, strain PCC10605 produced the highest titer C-PC of 0.652 g/g-DCW in the N1A2 medium with 10% light intensity and 16:8 light-period on day 7. PCC10605 accumulated 0.51 g-CPC/g-DCW at 20 g/L NaCl, while it grew normally in seawater with 30 g/L salinity, thus confirmed that PCC10605 was halo-tolerant strain. Besides, PCC10605 survived in 0.12 g/L phosphate medium that has never been reported. Finally, the purified C-PC exhibited DPPH, superoxide scavenging activity and antibacterial activity, which displayed 87.6%, and 18.7% removal of free radical, and 1.98 cm of inhibition zone for Escherichia coli.
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U2 - 10.1016/j.biortech.2021.125946
DO - 10.1016/j.biortech.2021.125946
M3 - Article
C2 - 34562714
AN - SCOPUS:85115401036
VL - 342
JO - Agricultural Wastes
JF - Agricultural Wastes
SN - 0960-8524
M1 - 125946
ER -