Production of Arachidonic and Eicosapentaenoic Acids by the Marine Oomycete Halophytophthora

Ka Lai Pang, Han Jia Lin, Hung Yun Lin, Yu Fen Huang, Yi-Min Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Polyunsaturated fatty acids (PUFAs) are fatty acids with more than one double bond in the chemical structure. Arachidonic acid (ARA, 20:4 (n-6)) and eicosapentaenoic acid (EPA, 22:5 (n-3)) are common PUFAs with beneficial health effects. Marine fish and meat are the main sources of omega-3 and omega-6 fatty acids in human’s diet, respectively. In particular, there is a general decline in fish catch, implicating the need for an alternative source of omega-3 fatty acids. Previous studies have examined the production of polyunsaturated fatty acids including ARA and EPA by various microorganisms, including microalgae, fungi, and thraustochytrids. In this study, the production of ARA and EPA by 10 isolates of four estuarine Halophytophthora species (Halophytophthora avicenniae, Halophytophthora polymorphica, Halophytophthora vesicula, and Halophytophthora spinosa var. spinosa) cultured from fallen mangrove leaves in Taiwan was examined. The yield of ARA ranged from 0.004 to 0.052 g/L with the highest yield of ARA obtained from H. spinosa var. spinosa IMB162, but no or a very low level of EPA was produced by IMB162. For EPA production by Halophytophthora spp., the yield ranged from 0 to 0.047 g/L. Percentage of ARA in total fatty acid ranged between 7.16 and 25.02 %. One-way ANOVA analysis using Tukey Test (p ≥ 0.05) suggested that there is significant difference in the percentage of EPA in total fatty acid produced by the isolates, which ranged from 0.01 to 18.42 %. BODIPY 505/515 fluorescent staining suggests that lipid bodies were evenly distributed in the mycelia of Halophytophthora species.

Original languageEnglish
Pages (from-to)121-129
Number of pages9
JournalMarine Biotechnology
Volume17
Issue number2
DOIs
Publication statusPublished - 2015 Jan 1

Fingerprint

Oomycetes
Arachidonic Acids
eicosapentaenoic acid
arachidonic acid
polyunsaturated fatty acids
Fatty Acids
fatty acid
Omega-3 Fatty Acids
fatty acids
Unsaturated Fatty Acids
acid
Fishes
Omega-6 Fatty Acids
Microalgae
Eicosapentaenoic Acid
lipid bodies
Mycelium
omega-6 fatty acids
microalgae
Taiwan

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Aquatic Science

Cite this

Pang, Ka Lai ; Lin, Han Jia ; Lin, Hung Yun ; Huang, Yu Fen ; Chen, Yi-Min. / Production of Arachidonic and Eicosapentaenoic Acids by the Marine Oomycete Halophytophthora. In: Marine Biotechnology. 2015 ; Vol. 17, No. 2. pp. 121-129.
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abstract = "Polyunsaturated fatty acids (PUFAs) are fatty acids with more than one double bond in the chemical structure. Arachidonic acid (ARA, 20:4 (n-6)) and eicosapentaenoic acid (EPA, 22:5 (n-3)) are common PUFAs with beneficial health effects. Marine fish and meat are the main sources of omega-3 and omega-6 fatty acids in human’s diet, respectively. In particular, there is a general decline in fish catch, implicating the need for an alternative source of omega-3 fatty acids. Previous studies have examined the production of polyunsaturated fatty acids including ARA and EPA by various microorganisms, including microalgae, fungi, and thraustochytrids. In this study, the production of ARA and EPA by 10 isolates of four estuarine Halophytophthora species (Halophytophthora avicenniae, Halophytophthora polymorphica, Halophytophthora vesicula, and Halophytophthora spinosa var. spinosa) cultured from fallen mangrove leaves in Taiwan was examined. The yield of ARA ranged from 0.004 to 0.052 g/L with the highest yield of ARA obtained from H. spinosa var. spinosa IMB162, but no or a very low level of EPA was produced by IMB162. For EPA production by Halophytophthora spp., the yield ranged from 0 to 0.047 g/L. Percentage of ARA in total fatty acid ranged between 7.16 and 25.02 {\%}. One-way ANOVA analysis using Tukey Test (p ≥ 0.05) suggested that there is significant difference in the percentage of EPA in total fatty acid produced by the isolates, which ranged from 0.01 to 18.42 {\%}. BODIPY 505/515 fluorescent staining suggests that lipid bodies were evenly distributed in the mycelia of Halophytophthora species.",
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Production of Arachidonic and Eicosapentaenoic Acids by the Marine Oomycete Halophytophthora. / Pang, Ka Lai; Lin, Han Jia; Lin, Hung Yun; Huang, Yu Fen; Chen, Yi-Min.

In: Marine Biotechnology, Vol. 17, No. 2, 01.01.2015, p. 121-129.

Research output: Contribution to journalArticle

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