TY - JOUR
T1 - Production of phycobiliprotein and carotenoid by efficient extraction from Thermosynechococcus sp. CL-1 cultivation in swine wastewater
AU - Narindri Rara Winayu, Birgitta
AU - Tung Lai, Ko
AU - Ta Hsueh, Hsin
AU - Chu, Hsin
N1 - Funding Information:
The authors acknowledge appreciatively to the National Science Council, Taiwan , for the financial support on the research with project number of MOST (109-2221-E-006-108).
Publisher Copyright:
© 2020 Elsevier Ltd
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - In this study, the performance of TCL-1 cultivation in swine wastewater was observed under various light intensity, treatment type of swine wastewater, and initial biomass concentration. Furthermore, pigments production (phycobiliprotein and carotenoid), was the main target in this study along with optimum extraction method. Under the cultivation in the anoxic treated swine wastewater (ATSW), highest biomass increment (1.001 ± 0.104 g/L) was achieved with 2 g/L initial biomass concentration and 1,000 µE/m2/s light intensity whereas cultivation in the anoxic and aerobic treated swine wastewater (AATSW) presented better performance on pigments production with the highest production in allophycocyanin which reached 12.07 ± 0.3% dwc. Extraction time and ultrasonication have significant influence on the phycobiliprotein extraction, yet different temperature and incubation time give similar extraction result for β-carotene. Carotenoids production with AATSW cultivation were two times higher than the cultivation in ATSW. However, ammonium-N degradation was performed better in the ATSW cultivation.
AB - In this study, the performance of TCL-1 cultivation in swine wastewater was observed under various light intensity, treatment type of swine wastewater, and initial biomass concentration. Furthermore, pigments production (phycobiliprotein and carotenoid), was the main target in this study along with optimum extraction method. Under the cultivation in the anoxic treated swine wastewater (ATSW), highest biomass increment (1.001 ± 0.104 g/L) was achieved with 2 g/L initial biomass concentration and 1,000 µE/m2/s light intensity whereas cultivation in the anoxic and aerobic treated swine wastewater (AATSW) presented better performance on pigments production with the highest production in allophycocyanin which reached 12.07 ± 0.3% dwc. Extraction time and ultrasonication have significant influence on the phycobiliprotein extraction, yet different temperature and incubation time give similar extraction result for β-carotene. Carotenoids production with AATSW cultivation were two times higher than the cultivation in ATSW. However, ammonium-N degradation was performed better in the ATSW cultivation.
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U2 - 10.1016/j.biortech.2020.124125
DO - 10.1016/j.biortech.2020.124125
M3 - Article
C2 - 32977095
AN - SCOPUS:85091237409
SN - 0960-8524
VL - 319
JO - Agricultural Wastes
JF - Agricultural Wastes
M1 - 124125
ER -
