Profiling of influenza viruses by high-throughput carbohydrate membrane array

Weng I. Lao, Ya Fang Wang, Yu Dai Kuo, Chun Hung Lin, Tsung Chain Chang, Ih Jen Su, Jen-Ren Wang, Chuan-Fa Chang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Carbohydrate-protein interactions participate in many biological functions. To characterize the binding interactions represents a longstanding challenge. Method: We developed a glycan membrane array to study the interactions of carbohydrates with lectins, proteins and viruses, including 17 lectins, four antibodies (that are specific to different Lewis antigens), one recombinant H5N1 hemagglutinin, and five influenza B clinical isolates. Results: The results were found to be comparable to previous reports, indicating the efficacy and reliability of our developed method. Conclusion: This carbohydrate membrane array represents a convenient, reliable and low-cost method to examine the carbohydrate-binding features of various proteins, high-throughput drug screening and the glycan-binding surveillance of influenza viruses.

Original languageEnglish
Pages (from-to)283-296
Number of pages14
JournalFuture Medicinal Chemistry
Volume3
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

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Orthomyxoviridae
Carbohydrates
Membranes
Lectins
Polysaccharides
Preclinical Drug Evaluations
Proteins
Hemagglutinins
Human Influenza
Viruses
Antigens
Costs and Cost Analysis
Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

Cite this

Lao, Weng I. ; Wang, Ya Fang ; Kuo, Yu Dai ; Lin, Chun Hung ; Chang, Tsung Chain ; Su, Ih Jen ; Wang, Jen-Ren ; Chang, Chuan-Fa. / Profiling of influenza viruses by high-throughput carbohydrate membrane array. In: Future Medicinal Chemistry. 2011 ; Vol. 3, No. 3. pp. 283-296.
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Profiling of influenza viruses by high-throughput carbohydrate membrane array. / Lao, Weng I.; Wang, Ya Fang; Kuo, Yu Dai; Lin, Chun Hung; Chang, Tsung Chain; Su, Ih Jen; Wang, Jen-Ren; Chang, Chuan-Fa.

In: Future Medicinal Chemistry, Vol. 3, No. 3, 01.03.2011, p. 283-296.

Research output: Contribution to journalArticle

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AU - Lao, Weng I.

AU - Wang, Ya Fang

AU - Kuo, Yu Dai

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AU - Su, Ih Jen

AU - Wang, Jen-Ren

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