Profiling of influenza viruses by high-throughput carbohydrate membrane array

Weng I. Lao, Ya Fang Wang, Yu Dai Kuo, Chun Hung Lin, Tsung Chain Chang, Ih Jen Su, Jen Ren Wang, Chuan Fa Chang

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background: Carbohydrate-protein interactions participate in many biological functions. To characterize the binding interactions represents a longstanding challenge. Method: We developed a glycan membrane array to study the interactions of carbohydrates with lectins, proteins and viruses, including 17 lectins, four antibodies (that are specific to different Lewis antigens), one recombinant H5N1 hemagglutinin, and five influenza B clinical isolates. Results: The results were found to be comparable to previous reports, indicating the efficacy and reliability of our developed method. Conclusion: This carbohydrate membrane array represents a convenient, reliable and low-cost method to examine the carbohydrate-binding features of various proteins, high-throughput drug screening and the glycan-binding surveillance of influenza viruses.

Original languageEnglish
Pages (from-to)283-296
Number of pages14
JournalFuture Medicinal Chemistry
Volume3
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Drug Discovery

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