Progenitor cells derived from drain waste product of open-heart surgery in children

Tak Wah Wong; Chung Dann Kan; Wen Tai Chiu; Kin Lam Fok; Ye Chun Ruan; Xiaohua Jiang; Junjiang Chen; Chiu Ching Kao; I. Yu Chen; Hui Chun Lin; Chia Hsuan Chou; Chou Wen Lin; Chun Keung Yu; Stephanie Tsao; Yi Ping Lee; Hsiao Chang Chan; Jieh Neng Wang

doi:10.3390/jcm8071028

Progenitor cells derived from drain waste product of open-heart surgery in children

Tak Wah Wong, Chung Dann Kan, Wen Tai Chiu, Kin Lam Fok, Ye Chun Ruan, Xiaohua Jiang, Junjiang Chen, Chiu Ching Kao, I. Yu Chen, Hui Chun Lin, Chia Hsuan Chou, Chou Wen Lin, Chun Keung Yu, Stephanie Tsao, Yi Ping Lee, Hsiao Chang Chan, Jieh Neng Wang

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Human cardiac progenitor cells isolated from the same host may have advantages over other sources of stem cells. The aim of this study is to establish a new source of human progenitor cells collected from a waste product, pericardiac effusion fluid, after open-heart surgery in children with congenital heart diseases. The fluid was collected every 24 h for 2 days after surgery in 37 children. Mononuclear cells were isolated and expanded in vitro. These pericardial effusion-derived progenitor cells (PEPCs) exhibiting cardiogenic lineage markers, were highly proliferative and enhanced angiogenesis in vitro. Three weeks after stem cell transplantation into the ischemic heart in mice, cardiac ejection fraction was improved significantly without detectable progenitor cells. Gene expression profiles of the repaired hearts revealed activation of several known repair mechanisms including paracrine effects, cell migration, and angiogenesis. These progenitor cells may have the potential for heart regeneration.

Original language	English
Article number	1028
Journal	Journal of Clinical Medicine
Volume	8
Issue number	7
DOIs	https://doi.org/10.3390/jcm8071028
Publication status	Published - 2019 Jul

All Science Journal Classification (ASJC) codes

General Medicine

Access to Document

10.3390/jcm8071028

Cite this

Wong, T. W., Kan, C. D., Chiu, W. T., Fok, K. L., Ruan, Y. C., Jiang, X., Chen, J., Kao, C. C., Chen, I. Y., Lin, H. C., Chou, C. H., Lin, C. W., Yu, C. K., Tsao, S., Lee, Y. P., Chan, H. C., & Wang, J. N. (2019). Progenitor cells derived from drain waste product of open-heart surgery in children. Journal of Clinical Medicine, 8(7), Article 1028. https://doi.org/10.3390/jcm8071028

@article{83f368ee901e49b688e250b2c06743f5,

title = "Progenitor cells derived from drain waste product of open-heart surgery in children",

abstract = "Human cardiac progenitor cells isolated from the same host may have advantages over other sources of stem cells. The aim of this study is to establish a new source of human progenitor cells collected from a waste product, pericardiac effusion fluid, after open-heart surgery in children with congenital heart diseases. The fluid was collected every 24 h for 2 days after surgery in 37 children. Mononuclear cells were isolated and expanded in vitro. These pericardial effusion-derived progenitor cells (PEPCs) exhibiting cardiogenic lineage markers, were highly proliferative and enhanced angiogenesis in vitro. Three weeks after stem cell transplantation into the ischemic heart in mice, cardiac ejection fraction was improved significantly without detectable progenitor cells. Gene expression profiles of the repaired hearts revealed activation of several known repair mechanisms including paracrine effects, cell migration, and angiogenesis. These progenitor cells may have the potential for heart regeneration.",

author = "Wong, {Tak Wah} and Kan, {Chung Dann} and Chiu, {Wen Tai} and Fok, {Kin Lam} and Ruan, {Ye Chun} and Xiaohua Jiang and Junjiang Chen and Kao, {Chiu Ching} and Chen, {I. Yu} and Lin, {Hui Chun} and Chou, {Chia Hsuan} and Lin, {Chou Wen} and Yu, {Chun Keung} and Stephanie Tsao and Lee, {Yi Ping} and Chan, {Hsiao Chang} and Wang, {Jieh Neng}",

note = "Publisher Copyright: {\textcopyright} 2019 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2019",

month = jul,

doi = "10.3390/jcm8071028",

language = "English",

volume = "8",

journal = "Journal of Clinical Medicine",

issn = "2077-0383",

number = "7",

}

TY - JOUR

T1 - Progenitor cells derived from drain waste product of open-heart surgery in children

AU - Wong, Tak Wah

AU - Kan, Chung Dann

AU - Chiu, Wen Tai

AU - Fok, Kin Lam

AU - Ruan, Ye Chun

AU - Jiang, Xiaohua

AU - Chen, Junjiang

AU - Kao, Chiu Ching

AU - Chen, I. Yu

AU - Lin, Hui Chun

AU - Chou, Chia Hsuan

AU - Lin, Chou Wen

AU - Yu, Chun Keung

AU - Tsao, Stephanie

AU - Lee, Yi Ping

AU - Chan, Hsiao Chang

AU - Wang, Jieh Neng

PY - 2019/7

Y1 - 2019/7

N2 - Human cardiac progenitor cells isolated from the same host may have advantages over other sources of stem cells. The aim of this study is to establish a new source of human progenitor cells collected from a waste product, pericardiac effusion fluid, after open-heart surgery in children with congenital heart diseases. The fluid was collected every 24 h for 2 days after surgery in 37 children. Mononuclear cells were isolated and expanded in vitro. These pericardial effusion-derived progenitor cells (PEPCs) exhibiting cardiogenic lineage markers, were highly proliferative and enhanced angiogenesis in vitro. Three weeks after stem cell transplantation into the ischemic heart in mice, cardiac ejection fraction was improved significantly without detectable progenitor cells. Gene expression profiles of the repaired hearts revealed activation of several known repair mechanisms including paracrine effects, cell migration, and angiogenesis. These progenitor cells may have the potential for heart regeneration.

AB - Human cardiac progenitor cells isolated from the same host may have advantages over other sources of stem cells. The aim of this study is to establish a new source of human progenitor cells collected from a waste product, pericardiac effusion fluid, after open-heart surgery in children with congenital heart diseases. The fluid was collected every 24 h for 2 days after surgery in 37 children. Mononuclear cells were isolated and expanded in vitro. These pericardial effusion-derived progenitor cells (PEPCs) exhibiting cardiogenic lineage markers, were highly proliferative and enhanced angiogenesis in vitro. Three weeks after stem cell transplantation into the ischemic heart in mice, cardiac ejection fraction was improved significantly without detectable progenitor cells. Gene expression profiles of the repaired hearts revealed activation of several known repair mechanisms including paracrine effects, cell migration, and angiogenesis. These progenitor cells may have the potential for heart regeneration.

UR - http://www.scopus.com/inward/record.url?scp=85112741654&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85112741654&partnerID=8YFLogxK

U2 - 10.3390/jcm8071028

DO - 10.3390/jcm8071028

M3 - Article

AN - SCOPUS:85112741654

SN - 2077-0383

VL - 8

JO - Journal of Clinical Medicine

JF - Journal of Clinical Medicine

IS - 7

M1 - 1028

ER -

Progenitor cells derived from drain waste product of open-heart surgery in children

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this