Prognostic role of tumoral PDL1 expression and peritumoral FoxP3+ lymphocytes in vulvar melanomas

Agata Chłopik, M. Angelica Selim, Yan Peng, Cheng Lin Wu, Gemma Tell-Marti, Kristen M. Paral, Sara C. Shalin, Stefan Kraft, Chao-Kai Hsu, Christopher R. Shea, Susana Puig, Maria Teresa Fernandez-Figueras, Wojciech Biernat, Janusz Ryś, Andrzej Marszalek, Mai P. Hoang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The prognostic role of PDL1 expression, CD8+ and FoxP3+ lymphocytes in vulvar melanomas has not been studied. We correlated PDL1 expression and CD8+ and FoxP3+ immune infiltrates with clinicopathologic variables and patient outcomes in a series of 75 vulvar melanomas. Tumoral PDL1 expression (>5%) was seen in 23% of cases. By Fisher exact test, PDL1 expression and peritumoral FoxP3+ lymphocytes significantly correlated with less disease-specific death. By linear regression analysis, correlations between tumoral PDL1 expression with the density of tumoral CD8+ and peritumoral CD8+ lymphocytes, tumoral FoxP3+ with tumoral CD8+ lymphocytes, and peritumoral FoxP3+ with peritumoral CD8+ lymphocytes were observed. By univariate analyses, tumor thickness >4 mm predicted poorer progression-free survival, melanoma-specific survival, and overall survival. PDL1 expression >5% and peritumoral CD8+, peritumoral FoxP3+, and tumoral FoxP3+ lymphocytes correlated with better overall survival. By multivariate analyses, high peritumoral FoxP3+ lymphocytes independently predicted better melanoma-specific survival (P =.023), and tumor thickness independently predicted poorer progression-free survival (P =.05) and overall survival (P =.039). In conclusion, our study shows that, independent from tumor thickness, an increased density of peritumoral FoxP3+ lymphocytes may positively impact survival in a subset of vulvar melanomas. Tumoral PDL1 expression correlated with tumoral as well as peritumoral CD8+ and FoxP3+ lymphocytes, supportive of an adaptive immune response. Although the frequency of PDL1 expression is low in vulvar melanoma, its expression may identify a subset of vulvar melanoma that might respond to immunotherapy.

Original languageEnglish
Pages (from-to)176-183
Number of pages8
JournalHuman Pathology
Volume73
DOIs
Publication statusPublished - 2018 Mar 1

Fingerprint

Melanoma
Lymphocytes
Survival
Disease-Free Survival
Neoplasms
Adaptive Immunity
Immunotherapy
Linear Models
Multivariate Analysis
Regression Analysis

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Chłopik, Agata ; Selim, M. Angelica ; Peng, Yan ; Wu, Cheng Lin ; Tell-Marti, Gemma ; Paral, Kristen M. ; Shalin, Sara C. ; Kraft, Stefan ; Hsu, Chao-Kai ; Shea, Christopher R. ; Puig, Susana ; Fernandez-Figueras, Maria Teresa ; Biernat, Wojciech ; Ryś, Janusz ; Marszalek, Andrzej ; Hoang, Mai P. / Prognostic role of tumoral PDL1 expression and peritumoral FoxP3+ lymphocytes in vulvar melanomas. In: Human Pathology. 2018 ; Vol. 73. pp. 176-183.
@article{9cc7d8a2abb8452faad32e18ced0adab,
title = "Prognostic role of tumoral PDL1 expression and peritumoral FoxP3+ lymphocytes in vulvar melanomas",
abstract = "The prognostic role of PDL1 expression, CD8+ and FoxP3+ lymphocytes in vulvar melanomas has not been studied. We correlated PDL1 expression and CD8+ and FoxP3+ immune infiltrates with clinicopathologic variables and patient outcomes in a series of 75 vulvar melanomas. Tumoral PDL1 expression (>5{\%}) was seen in 23{\%} of cases. By Fisher exact test, PDL1 expression and peritumoral FoxP3+ lymphocytes significantly correlated with less disease-specific death. By linear regression analysis, correlations between tumoral PDL1 expression with the density of tumoral CD8+ and peritumoral CD8+ lymphocytes, tumoral FoxP3+ with tumoral CD8+ lymphocytes, and peritumoral FoxP3+ with peritumoral CD8+ lymphocytes were observed. By univariate analyses, tumor thickness >4 mm predicted poorer progression-free survival, melanoma-specific survival, and overall survival. PDL1 expression >5{\%} and peritumoral CD8+, peritumoral FoxP3+, and tumoral FoxP3+ lymphocytes correlated with better overall survival. By multivariate analyses, high peritumoral FoxP3+ lymphocytes independently predicted better melanoma-specific survival (P =.023), and tumor thickness independently predicted poorer progression-free survival (P =.05) and overall survival (P =.039). In conclusion, our study shows that, independent from tumor thickness, an increased density of peritumoral FoxP3+ lymphocytes may positively impact survival in a subset of vulvar melanomas. Tumoral PDL1 expression correlated with tumoral as well as peritumoral CD8+ and FoxP3+ lymphocytes, supportive of an adaptive immune response. Although the frequency of PDL1 expression is low in vulvar melanoma, its expression may identify a subset of vulvar melanoma that might respond to immunotherapy.",
author = "Agata Chłopik and Selim, {M. Angelica} and Yan Peng and Wu, {Cheng Lin} and Gemma Tell-Marti and Paral, {Kristen M.} and Shalin, {Sara C.} and Stefan Kraft and Chao-Kai Hsu and Shea, {Christopher R.} and Susana Puig and Fernandez-Figueras, {Maria Teresa} and Wojciech Biernat and Janusz Ryś and Andrzej Marszalek and Hoang, {Mai P.}",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.humpath.2017.12.022",
language = "English",
volume = "73",
pages = "176--183",
journal = "Human Pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",

}

Chłopik, A, Selim, MA, Peng, Y, Wu, CL, Tell-Marti, G, Paral, KM, Shalin, SC, Kraft, S, Hsu, C-K, Shea, CR, Puig, S, Fernandez-Figueras, MT, Biernat, W, Ryś, J, Marszalek, A & Hoang, MP 2018, 'Prognostic role of tumoral PDL1 expression and peritumoral FoxP3+ lymphocytes in vulvar melanomas', Human Pathology, vol. 73, pp. 176-183. https://doi.org/10.1016/j.humpath.2017.12.022

Prognostic role of tumoral PDL1 expression and peritumoral FoxP3+ lymphocytes in vulvar melanomas. / Chłopik, Agata; Selim, M. Angelica; Peng, Yan; Wu, Cheng Lin; Tell-Marti, Gemma; Paral, Kristen M.; Shalin, Sara C.; Kraft, Stefan; Hsu, Chao-Kai; Shea, Christopher R.; Puig, Susana; Fernandez-Figueras, Maria Teresa; Biernat, Wojciech; Ryś, Janusz; Marszalek, Andrzej; Hoang, Mai P.

In: Human Pathology, Vol. 73, 01.03.2018, p. 176-183.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Prognostic role of tumoral PDL1 expression and peritumoral FoxP3+ lymphocytes in vulvar melanomas

AU - Chłopik, Agata

AU - Selim, M. Angelica

AU - Peng, Yan

AU - Wu, Cheng Lin

AU - Tell-Marti, Gemma

AU - Paral, Kristen M.

AU - Shalin, Sara C.

AU - Kraft, Stefan

AU - Hsu, Chao-Kai

AU - Shea, Christopher R.

AU - Puig, Susana

AU - Fernandez-Figueras, Maria Teresa

AU - Biernat, Wojciech

AU - Ryś, Janusz

AU - Marszalek, Andrzej

AU - Hoang, Mai P.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - The prognostic role of PDL1 expression, CD8+ and FoxP3+ lymphocytes in vulvar melanomas has not been studied. We correlated PDL1 expression and CD8+ and FoxP3+ immune infiltrates with clinicopathologic variables and patient outcomes in a series of 75 vulvar melanomas. Tumoral PDL1 expression (>5%) was seen in 23% of cases. By Fisher exact test, PDL1 expression and peritumoral FoxP3+ lymphocytes significantly correlated with less disease-specific death. By linear regression analysis, correlations between tumoral PDL1 expression with the density of tumoral CD8+ and peritumoral CD8+ lymphocytes, tumoral FoxP3+ with tumoral CD8+ lymphocytes, and peritumoral FoxP3+ with peritumoral CD8+ lymphocytes were observed. By univariate analyses, tumor thickness >4 mm predicted poorer progression-free survival, melanoma-specific survival, and overall survival. PDL1 expression >5% and peritumoral CD8+, peritumoral FoxP3+, and tumoral FoxP3+ lymphocytes correlated with better overall survival. By multivariate analyses, high peritumoral FoxP3+ lymphocytes independently predicted better melanoma-specific survival (P =.023), and tumor thickness independently predicted poorer progression-free survival (P =.05) and overall survival (P =.039). In conclusion, our study shows that, independent from tumor thickness, an increased density of peritumoral FoxP3+ lymphocytes may positively impact survival in a subset of vulvar melanomas. Tumoral PDL1 expression correlated with tumoral as well as peritumoral CD8+ and FoxP3+ lymphocytes, supportive of an adaptive immune response. Although the frequency of PDL1 expression is low in vulvar melanoma, its expression may identify a subset of vulvar melanoma that might respond to immunotherapy.

AB - The prognostic role of PDL1 expression, CD8+ and FoxP3+ lymphocytes in vulvar melanomas has not been studied. We correlated PDL1 expression and CD8+ and FoxP3+ immune infiltrates with clinicopathologic variables and patient outcomes in a series of 75 vulvar melanomas. Tumoral PDL1 expression (>5%) was seen in 23% of cases. By Fisher exact test, PDL1 expression and peritumoral FoxP3+ lymphocytes significantly correlated with less disease-specific death. By linear regression analysis, correlations between tumoral PDL1 expression with the density of tumoral CD8+ and peritumoral CD8+ lymphocytes, tumoral FoxP3+ with tumoral CD8+ lymphocytes, and peritumoral FoxP3+ with peritumoral CD8+ lymphocytes were observed. By univariate analyses, tumor thickness >4 mm predicted poorer progression-free survival, melanoma-specific survival, and overall survival. PDL1 expression >5% and peritumoral CD8+, peritumoral FoxP3+, and tumoral FoxP3+ lymphocytes correlated with better overall survival. By multivariate analyses, high peritumoral FoxP3+ lymphocytes independently predicted better melanoma-specific survival (P =.023), and tumor thickness independently predicted poorer progression-free survival (P =.05) and overall survival (P =.039). In conclusion, our study shows that, independent from tumor thickness, an increased density of peritumoral FoxP3+ lymphocytes may positively impact survival in a subset of vulvar melanomas. Tumoral PDL1 expression correlated with tumoral as well as peritumoral CD8+ and FoxP3+ lymphocytes, supportive of an adaptive immune response. Although the frequency of PDL1 expression is low in vulvar melanoma, its expression may identify a subset of vulvar melanoma that might respond to immunotherapy.

UR - http://www.scopus.com/inward/record.url?scp=85042188109&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042188109&partnerID=8YFLogxK

U2 - 10.1016/j.humpath.2017.12.022

DO - 10.1016/j.humpath.2017.12.022

M3 - Article

VL - 73

SP - 176

EP - 183

JO - Human Pathology

JF - Human Pathology

SN - 0046-8177

ER -