Propagation and immunological characterization of coxsackievirus A10 in a serum-free HEK293A cell culture system

Sheng Chieh Lien, Yu Sheng Shen, Hsiao Yu Lin, Shang Rung Wu, Chih Yeu Fang, Chi Hsun Chen, Yi An Chen, Pele Choi Sing Chong, Ming Hsi Huang, Yen Hung Chow, Jen Ren Wang, Suh Chin Wu, Chia Chyi Liu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Coxsackievirus A10 (CVA10) is one of enteroviral pathogens that cause the hand, foot, and mouth disease (HFMD). Since CVA10 was reported to be not easily propagated in the Vero cell culture, a feasible manufacture process for producing formalin-inactivated CVA10 vaccine is urgently needed. Several cell lines that commonly used for viral vaccine production was tested for CVA10 (M2014 strain) culture in this study, and our result showed that CVA10 could be easily propagated in the HEK293A cells. A serum-free HEK293A cell culture system was developed for CVA10 production and the yields have reached over 108 TCID50/mL. The biochemical and immunogenic properties of CVA10 particles obtained from this serum-free HEK293A culture were identical to our previous study. Two major particles of CVA10 were separated by ultracentrifugation, and only the infectious mature particles were capable of inducing CVA10 neutralizing antibody responses in the mouse immunogenicity studies. Additionally, we found that coxsackievirus A6 and enterovirus A71 could also be easily propagated using this serum-free HEK293A cell culture system. Our results provide a solution to overcome the obstacle in the propagation of CVA10 and facilitate the development of multivalent vaccines for prevention of HFMD.

Original languageEnglish
Article number199101
JournalVirus Research
Volume329
DOIs
Publication statusPublished - 2023 May

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases
  • Cancer Research

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