Properties of BKCa channels in oral keratinocytes

Dar-Bin Shieh, S. R. Yang, X. Y. Shi, Y. N. Wu, Sheng-Nan Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Keratinocytes are important for epithelial antimicrobial barrier function. The activity of ion channels can affect the proliferation of keratinocytes. Little is known about Ca2+-activated K+ currents in these cells. Ion currents in normal human oral keratinocytes were characterized with a patch-clamp technique. In whole-cell configuration, depolarizing pulses evoked K+ outward currents (iK) in oral keratinocytes. Iberiotoxin (200 nM) and paxilline (1 μM) suppressed IK; however, neither apamin (200 nM) nor 5-hydroxydecanoate (30 μM) had any effects on it. Caffeic acid phenethyl ester, a compound of honeybee propolis, increased I K with an EC50 value of 12.8 ± 1.2 μM. In inside-out patches, a BKCa channel was observed in keratinocytes, but not in oral squamous carcinoma (OCE-M1) cells. Caffeic acid phenethyl ester or cinnamyl-3,4-dihydroxy-α-cyanocinnamate applied to the intracellular surface of a detached patch increased BKCa-channel activity. The results demonstrate that the properties of BKCa channels in normal human oral keratinocytes are similar to those described in other types of cells. Caffeic acid derivatives can also stimulate BKCa-channel activity directly.

Original languageEnglish
Pages (from-to)468-473
Number of pages6
JournalJournal of Dental Research
Volume84
Issue number5
DOIs
Publication statusPublished - 2005 May 1

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Keratinocytes
Propolis
Apamin
Patch-Clamp Techniques
Ion Channels
Squamous Cell Carcinoma
Ions

All Science Journal Classification (ASJC) codes

  • Dentistry(all)

Cite this

Shieh, Dar-Bin ; Yang, S. R. ; Shi, X. Y. ; Wu, Y. N. ; Wu, Sheng-Nan. / Properties of BKCa channels in oral keratinocytes. In: Journal of Dental Research. 2005 ; Vol. 84, No. 5. pp. 468-473.
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Properties of BKCa channels in oral keratinocytes. / Shieh, Dar-Bin; Yang, S. R.; Shi, X. Y.; Wu, Y. N.; Wu, Sheng-Nan.

In: Journal of Dental Research, Vol. 84, No. 5, 01.05.2005, p. 468-473.

Research output: Contribution to journalArticle

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