Prostate cancer-specific thermo-responsive polymer-coated iron oxide nanoparticles

Aniket S. Wadajkar, Jyothi U. Menon, Yuh Shyan Tsai, Crystal Gore, Timothy Dobin, Leah Gandee, Kim Kangasniemi, Masaya Takahashi, Bikash Manandhar, Jung Mo Ahn, Jer Tsong Hsieh, Kytai T. Nguyen

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Thermo-responsive poly(N-isopropylacrylamide-acrylamide-allylamine)-coated magnetic nanoparticles (PMNPs) were developed and conjugated with prostate cancer-specific R11 peptides for active targeting and imaging of prostate cancer. The stable nanoparticles with an average diameter of 100 nm and surface charge of -27.0 mV, had a lower critical solution temperature of 40 °C. Magnetic characterization showed that the nanoparticles can be recruited using a magnetic field and possess superparamagnetic behavior even after R11 conjugation. In vitro cell studies demonstrated that R11-conjugated PMNPs (R11-PMNPs) were compatible with human dermal fibroblasts and normal prostate epithelial cells to all tested concentrations up to 500 μg/ml after 24 h of incubation. Moreover, the nanoparticles were taken up by prostate cancer cells (PC3 and LNCaP) in a dose-dependent manner, which was higher in case of R11-PMNPs than PMNPs. Further, in vivo biodistribution of the nanoparticles showed significantly more R11-PMNPs accumulation in tumors than other vital organs unlike PMNPs without R11 conjugation. Moreover, R11-PMNPs decreased 30% magnetic resonance T2 signal intensity in tumors in vivo compared to 0% decrease with PMNPs. These results indicate great potential of R11-PMPs as platform technology to target and monitor prostate cancers for diagnostic and therapeutic applications.

Original languageEnglish
Pages (from-to)3618-3625
Number of pages8
JournalBiomaterials
Volume34
Issue number14
DOIs
Publication statusPublished - 2013 May

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Ceramics and Composites
  • Biomaterials
  • Mechanics of Materials

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