Protein microarrays: Flexible tools for scientific innovation

Johnathan Neiswinger, Ijeoma Uzoma, Eric Cox, Heesool Rho, Guang Song, Corry Paul, Jun Seop Jeong, Kuan Yi Lu, Chien Sheng Chen, Heng Zhu

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2 Citations (Scopus)

Abstract

Protein microarrays have emerged as a powerful tool for the scientific community, and their greatest advantage lies in the fact that thousands of reactions can be performed in a parallel and unbiased manner. The first high-density protein microarray, dubbed the “yeast proteome array,” consisted of approximately 5800 full-length yeast proteins and was initially used to identify protein-lipid interactions. Further assays were subsequently developed to allow measurement of protein-DNA, protein RNA, and protein-protein interactions, as well as four well-known posttranslational modifications: phosphorylation, acetylation, ubiquitylation, and SUMOylation. In this introduction, we describe the advent of high-density protein microarrays, as well as current methods for assessing a wide variety of protein interactions and posttranslational modifications.

Original languageEnglish
Pages (from-to)837-839
Number of pages3
JournalCold Spring Harbor Protocols
Volume2016
Issue number10
DOIs
Publication statusPublished - 2016 Oct

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All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Neiswinger, J., Uzoma, I., Cox, E., Rho, H., Song, G., Paul, C., Jeong, J. S., Lu, K. Y., Chen, C. S., & Zhu, H. (2016). Protein microarrays: Flexible tools for scientific innovation. Cold Spring Harbor Protocols, 2016(10), 837-839. https://doi.org/10.1101/pdb.top081471