Protein microarrays

Flexible tools for scientific innovation

Johnathan Neiswinger, Ijeoma Uzoma, Eric Cox, Heesool Rho, Guang Song, Corry Paul, Jun Seop Jeong, Kuan Yi Lu, Chien-Sheng Chen, Heng Zhu

Research output: Contribution to journalArticle

Abstract

Protein microarrays have emerged as a powerful tool for the scientific community, and their greatest advantage lies in the fact that thousands of reactions can be performed in a parallel and unbiased manner. The first high-density protein microarray, dubbed the “yeast proteome array,” consisted of approximately 5800 full-length yeast proteins and was initially used to identify protein-lipid interactions. Further assays were subsequently developed to allow measurement of protein-DNA, protein RNA, and protein-protein interactions, as well as four well-known posttranslational modifications: phosphorylation, acetylation, ubiquitylation, and SUMOylation. In this introduction, we describe the advent of high-density protein microarrays, as well as current methods for assessing a wide variety of protein interactions and posttranslational modifications.

Original languageEnglish
Pages (from-to)837-839
Number of pages3
JournalCold Spring Harbor Protocols
Volume2016
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

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Protein Array Analysis
Microarrays
Innovation
Proteins
Post Translational Protein Processing
Sumoylation
Fungal Proteins
Ubiquitination
Proteome
Acetylation
Phosphorylation
Yeasts
RNA
Lipids
Yeast
Assays
DNA

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Neiswinger, J., Uzoma, I., Cox, E., Rho, H., Song, G., Paul, C., ... Zhu, H. (2016). Protein microarrays: Flexible tools for scientific innovation. Cold Spring Harbor Protocols, 2016(10), 837-839. https://doi.org/10.1101/pdb.top081471
Neiswinger, Johnathan ; Uzoma, Ijeoma ; Cox, Eric ; Rho, Heesool ; Song, Guang ; Paul, Corry ; Jeong, Jun Seop ; Lu, Kuan Yi ; Chen, Chien-Sheng ; Zhu, Heng. / Protein microarrays : Flexible tools for scientific innovation. In: Cold Spring Harbor Protocols. 2016 ; Vol. 2016, No. 10. pp. 837-839.
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Neiswinger, J, Uzoma, I, Cox, E, Rho, H, Song, G, Paul, C, Jeong, JS, Lu, KY, Chen, C-S & Zhu, H 2016, 'Protein microarrays: Flexible tools for scientific innovation', Cold Spring Harbor Protocols, vol. 2016, no. 10, pp. 837-839. https://doi.org/10.1101/pdb.top081471

Protein microarrays : Flexible tools for scientific innovation. / Neiswinger, Johnathan; Uzoma, Ijeoma; Cox, Eric; Rho, Heesool; Song, Guang; Paul, Corry; Jeong, Jun Seop; Lu, Kuan Yi; Chen, Chien-Sheng; Zhu, Heng.

In: Cold Spring Harbor Protocols, Vol. 2016, No. 10, 01.10.2016, p. 837-839.

Research output: Contribution to journalArticle

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Neiswinger J, Uzoma I, Cox E, Rho H, Song G, Paul C et al. Protein microarrays: Flexible tools for scientific innovation. Cold Spring Harbor Protocols. 2016 Oct 1;2016(10):837-839. https://doi.org/10.1101/pdb.top081471