Protein preconcentration using nanofissures generated by nanoparticle-facilitaed electric breakdown at the junction gaps

Chun Ping Jen, Chen Chi Kuo, Pei Ju Chiang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Sample preconcentration is an important step to increase the accuracy of further detection, especially for the sample with extreme low concentration. Due to the overlapping of the electrical double layers in the nanochannel, the concentration polarization effect could be generated while applying an electric field. Therefore, a nonlinear electrokinetic flow is induced, which results in fast accumulation of proteins in front of the induced ionic depletion zone, so-called exclusion-enrichment effect. The main purpose of this work is to create nanofissures to achieve the preconcentration of proteins by the exclusion-enrichment effect. The sample of protein is driven by electroosmotic flow and accumulates at the specific location. In this study, the preconcentration chip for proteins was mainly fabricated by simple standard soft lithography with replica of polydimethylsiloxane (PDMS) and fast nanofissures formation by utilizing nanoparticle-facilitaed electric breakdown phenomenon. A novel strategy of nanofissures formation utilizing nanoparticles deposition at the junction gap between microchannels was proposed and dramatically decreased the required electric breakdown voltage in this study. The experimental results indicated that the sample of protein with extreme low concentration of 1 nM was concentrated to 1.5×104-fold in 60 min by the proposed chip herein.

Original languageEnglish
Title of host publication2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013
Publication statusPublished - 2013 Sep 2
Event2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013 - Barcelona, Spain
Duration: 2013 Apr 162013 Apr 18

Publication series

Name2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013

Other

Other2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013
CountrySpain
CityBarcelona
Period13-04-1613-04-18

Fingerprint

Electric breakdown
Nanoparticles
Proteins
Polydimethylsiloxane
Microchannels
Lithography
Electric fields
Polarization

All Science Journal Classification (ASJC) codes

  • Hardware and Architecture

Cite this

Jen, C. P., Kuo, C. C., & Chiang, P. J. (2013). Protein preconcentration using nanofissures generated by nanoparticle-facilitaed electric breakdown at the junction gaps. In 2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013 [6559408] (2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013).
Jen, Chun Ping ; Kuo, Chen Chi ; Chiang, Pei Ju. / Protein preconcentration using nanofissures generated by nanoparticle-facilitaed electric breakdown at the junction gaps. 2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013. 2013. (2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013).
@inproceedings{e4a0e96987724c77956382a38d9f8ecc,
title = "Protein preconcentration using nanofissures generated by nanoparticle-facilitaed electric breakdown at the junction gaps",
abstract = "Sample preconcentration is an important step to increase the accuracy of further detection, especially for the sample with extreme low concentration. Due to the overlapping of the electrical double layers in the nanochannel, the concentration polarization effect could be generated while applying an electric field. Therefore, a nonlinear electrokinetic flow is induced, which results in fast accumulation of proteins in front of the induced ionic depletion zone, so-called exclusion-enrichment effect. The main purpose of this work is to create nanofissures to achieve the preconcentration of proteins by the exclusion-enrichment effect. The sample of protein is driven by electroosmotic flow and accumulates at the specific location. In this study, the preconcentration chip for proteins was mainly fabricated by simple standard soft lithography with replica of polydimethylsiloxane (PDMS) and fast nanofissures formation by utilizing nanoparticle-facilitaed electric breakdown phenomenon. A novel strategy of nanofissures formation utilizing nanoparticles deposition at the junction gap between microchannels was proposed and dramatically decreased the required electric breakdown voltage in this study. The experimental results indicated that the sample of protein with extreme low concentration of 1 nM was concentrated to 1.5×104-fold in 60 min by the proposed chip herein.",
author = "Jen, {Chun Ping} and Kuo, {Chen Chi} and Chiang, {Pei Ju}",
year = "2013",
month = "9",
day = "2",
language = "English",
isbn = "9781467344777",
series = "2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013",
booktitle = "2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013",

}

Jen, CP, Kuo, CC & Chiang, PJ 2013, Protein preconcentration using nanofissures generated by nanoparticle-facilitaed electric breakdown at the junction gaps. in 2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013., 6559408, 2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013, 2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013, Barcelona, Spain, 13-04-16.

Protein preconcentration using nanofissures generated by nanoparticle-facilitaed electric breakdown at the junction gaps. / Jen, Chun Ping; Kuo, Chen Chi; Chiang, Pei Ju.

2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013. 2013. 6559408 (2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Protein preconcentration using nanofissures generated by nanoparticle-facilitaed electric breakdown at the junction gaps

AU - Jen, Chun Ping

AU - Kuo, Chen Chi

AU - Chiang, Pei Ju

PY - 2013/9/2

Y1 - 2013/9/2

N2 - Sample preconcentration is an important step to increase the accuracy of further detection, especially for the sample with extreme low concentration. Due to the overlapping of the electrical double layers in the nanochannel, the concentration polarization effect could be generated while applying an electric field. Therefore, a nonlinear electrokinetic flow is induced, which results in fast accumulation of proteins in front of the induced ionic depletion zone, so-called exclusion-enrichment effect. The main purpose of this work is to create nanofissures to achieve the preconcentration of proteins by the exclusion-enrichment effect. The sample of protein is driven by electroosmotic flow and accumulates at the specific location. In this study, the preconcentration chip for proteins was mainly fabricated by simple standard soft lithography with replica of polydimethylsiloxane (PDMS) and fast nanofissures formation by utilizing nanoparticle-facilitaed electric breakdown phenomenon. A novel strategy of nanofissures formation utilizing nanoparticles deposition at the junction gap between microchannels was proposed and dramatically decreased the required electric breakdown voltage in this study. The experimental results indicated that the sample of protein with extreme low concentration of 1 nM was concentrated to 1.5×104-fold in 60 min by the proposed chip herein.

AB - Sample preconcentration is an important step to increase the accuracy of further detection, especially for the sample with extreme low concentration. Due to the overlapping of the electrical double layers in the nanochannel, the concentration polarization effect could be generated while applying an electric field. Therefore, a nonlinear electrokinetic flow is induced, which results in fast accumulation of proteins in front of the induced ionic depletion zone, so-called exclusion-enrichment effect. The main purpose of this work is to create nanofissures to achieve the preconcentration of proteins by the exclusion-enrichment effect. The sample of protein is driven by electroosmotic flow and accumulates at the specific location. In this study, the preconcentration chip for proteins was mainly fabricated by simple standard soft lithography with replica of polydimethylsiloxane (PDMS) and fast nanofissures formation by utilizing nanoparticle-facilitaed electric breakdown phenomenon. A novel strategy of nanofissures formation utilizing nanoparticles deposition at the junction gap between microchannels was proposed and dramatically decreased the required electric breakdown voltage in this study. The experimental results indicated that the sample of protein with extreme low concentration of 1 nM was concentrated to 1.5×104-fold in 60 min by the proposed chip herein.

UR - http://www.scopus.com/inward/record.url?scp=84883126464&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883126464&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84883126464

SN - 9781467344777

T3 - 2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013

BT - 2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013

ER -

Jen CP, Kuo CC, Chiang PJ. Protein preconcentration using nanofissures generated by nanoparticle-facilitaed electric breakdown at the junction gaps. In 2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013. 2013. 6559408. (2013 Symposium on Design, Test, Integration and Packaging of MEMS/MOEMS, DTIP 2013).