Protein preconcentration using nanofractures generated by nanoparticle-assisted electric breakdown at junction gaps

Chun Ping Jen, Tamara G. Amstislavskaya, Chen Chi Kuo, Yu Hung Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Sample preconcentration is an important step that increases the accuracy of subsequent detection, especially for samples with extremely low concentrations. Due to the overlapping of electrical double layers in the nanofluidic channel, the concentration polarization effect can be generated by applying an electric field. Therefore, a nonlinear electrokinetic flow is induced, which results in the fast accumulation of proteins in front of the induced ionic depletion zone, the so-called exclusion-enrichment effect. Nanofractures were created in this work to preconcentrate proteins via the exclusion-enrichment effect. The protein sample was driven by electroosmotic flow and accumulated at a specific location. The preconcentration chip for proteins was fabricated using simple standard soft lithography with a polydimethylsiloxane replica. Nanofractures were formed by utilizing nanoparticle-assisted electric breakdown. The proposed method for nanofracture formation that utilizes nanoparticle deposition at the junction gap between microchannels greatly decreases the required electric breakdown voltage. The experimental results indicate that a protein sample with an extremely low concentration of 1 nM was concentrated to 1.5×10 4-fold in 60 min using the proposed chip.

Original languageEnglish
Article numbere102050
JournalPloS one
Volume9
Issue number7
DOIs
Publication statusPublished - 2014 Jul 15

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Protein preconcentration using nanofractures generated by nanoparticle-assisted electric breakdown at junction gaps'. Together they form a unique fingerprint.

  • Cite this