Protein transport into chloroplasts

Hsou Min Li, Chi Chou Chiu

Research output: Contribution to journalArticle

186 Citations (Scopus)

Abstract

Most proteins in chloroplasts are encoded by the nuclear genome and synthesized as precursors with N-terminal targeting signals called transit peptides. Novel machinery has evolved to specifically import these proteins from the cytosol into chloroplasts. This machinery consists of more than a dozen components located in and around the chloroplast envelope, including a pair of GTPase receptors, a β-barrel-type channel across the outer membrane, and an AAA+-type motor in the stroma. How individual components assemble into functional subcomplexes and the sequential steps of the translocation process are being mapped out. An increasing number of noncanonical import pathways, including a pathway with initial transport through the endomembrane system, is being revealed. Multiple levels of control on protein transport into chloroplasts have evolved, including the development of two receptor subfamilies, one for photosynthetic proteins and one for housekeeping proteins. The functions or expression levels of some translocon components are further adjusted according to plastid type, developmental stage, and metabolic conditions.

Original languageEnglish
Pages (from-to)157-180
Number of pages24
JournalAnnual Review of Plant Biology
Volume61
DOIs
Publication statusPublished - 2010 Jun 2

Fingerprint

protein transport
Protein Transport
Chloroplasts
chloroplasts
imports
Chloroplast Proteins
Housekeeping
Proteins
Plastids
proteins
GTP Phosphohydrolases
endomembrane system
Cytosol
receptors
guanosinetriphosphatase
cytosol
Genome
nuclear genome
plastids
Peptides

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Plant Science
  • Cell Biology

Cite this

@article{9cc8ba7fdc4a464eb09b6699501817d0,
title = "Protein transport into chloroplasts",
abstract = "Most proteins in chloroplasts are encoded by the nuclear genome and synthesized as precursors with N-terminal targeting signals called transit peptides. Novel machinery has evolved to specifically import these proteins from the cytosol into chloroplasts. This machinery consists of more than a dozen components located in and around the chloroplast envelope, including a pair of GTPase receptors, a β-barrel-type channel across the outer membrane, and an AAA+-type motor in the stroma. How individual components assemble into functional subcomplexes and the sequential steps of the translocation process are being mapped out. An increasing number of noncanonical import pathways, including a pathway with initial transport through the endomembrane system, is being revealed. Multiple levels of control on protein transport into chloroplasts have evolved, including the development of two receptor subfamilies, one for photosynthetic proteins and one for housekeeping proteins. The functions or expression levels of some translocon components are further adjusted according to plastid type, developmental stage, and metabolic conditions.",
author = "Li, {Hsou Min} and Chiu, {Chi Chou}",
year = "2010",
month = "6",
day = "2",
doi = "10.1146/annurev-arplant-042809-112222",
language = "English",
volume = "61",
pages = "157--180",
journal = "Annual Review of Plant Biology",
issn = "1543-5008",
publisher = "Annual Reviews Inc.",

}

Protein transport into chloroplasts. / Li, Hsou Min; Chiu, Chi Chou.

In: Annual Review of Plant Biology, Vol. 61, 02.06.2010, p. 157-180.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Protein transport into chloroplasts

AU - Li, Hsou Min

AU - Chiu, Chi Chou

PY - 2010/6/2

Y1 - 2010/6/2

N2 - Most proteins in chloroplasts are encoded by the nuclear genome and synthesized as precursors with N-terminal targeting signals called transit peptides. Novel machinery has evolved to specifically import these proteins from the cytosol into chloroplasts. This machinery consists of more than a dozen components located in and around the chloroplast envelope, including a pair of GTPase receptors, a β-barrel-type channel across the outer membrane, and an AAA+-type motor in the stroma. How individual components assemble into functional subcomplexes and the sequential steps of the translocation process are being mapped out. An increasing number of noncanonical import pathways, including a pathway with initial transport through the endomembrane system, is being revealed. Multiple levels of control on protein transport into chloroplasts have evolved, including the development of two receptor subfamilies, one for photosynthetic proteins and one for housekeeping proteins. The functions or expression levels of some translocon components are further adjusted according to plastid type, developmental stage, and metabolic conditions.

AB - Most proteins in chloroplasts are encoded by the nuclear genome and synthesized as precursors with N-terminal targeting signals called transit peptides. Novel machinery has evolved to specifically import these proteins from the cytosol into chloroplasts. This machinery consists of more than a dozen components located in and around the chloroplast envelope, including a pair of GTPase receptors, a β-barrel-type channel across the outer membrane, and an AAA+-type motor in the stroma. How individual components assemble into functional subcomplexes and the sequential steps of the translocation process are being mapped out. An increasing number of noncanonical import pathways, including a pathway with initial transport through the endomembrane system, is being revealed. Multiple levels of control on protein transport into chloroplasts have evolved, including the development of two receptor subfamilies, one for photosynthetic proteins and one for housekeeping proteins. The functions or expression levels of some translocon components are further adjusted according to plastid type, developmental stage, and metabolic conditions.

UR - http://www.scopus.com/inward/record.url?scp=77952506871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77952506871&partnerID=8YFLogxK

U2 - 10.1146/annurev-arplant-042809-112222

DO - 10.1146/annurev-arplant-042809-112222

M3 - Article

C2 - 20192748

AN - SCOPUS:77952506871

VL - 61

SP - 157

EP - 180

JO - Annual Review of Plant Biology

JF - Annual Review of Plant Biology

SN - 1543-5008

ER -