TY - JOUR
T1 - Proteins recovery from wet microalgae using liquid biphasic flotation (LBF)
AU - Phong, Win Nee
AU - Show, Pau Loke
AU - Teh, Wei Heng
AU - Teh, Tiong Xin
AU - Lim, Hilary Mae Yan
AU - Nazri, Nurul Shafira binti
AU - Tan, Chung Hong
AU - Chang, Jo Shu
AU - Ling, Tau Chuan
N1 - Funding Information:
This work is supported financially by SATU Joint Research Scheme ( RU018L-2016 , RU018O-2016 and RU018C-2016 ) from University of Malaya ( PG250-2015B , and RP031B-15AET ), Malaysia’s Fundamental Research Grant Scheme ( FRGS/1/2015/SG05/UNIM/03/1 ), Prototype Research Grant Scheme ( PRGS/2/2015/SG05/UNIM/03/1 ), Malaysia’s Ministry of Science, Technology, Innovation ( MOSTI 02-02-12-SF0256 ), Taiwan’s Ministry of Science and Technology ( 106-3113-E-006 -011 , 106-3113-E-006 -004CC2 , and 103-2221-E-006-190-MY3 ) and Taiwan’s Ministry of Education on Top University Grants.
Funding Information:
This work is supported financially by SATU Joint Research Scheme (RU018L-2016, RU018O-2016 and RU018C-2016) from University of Malaya (PG250-2015B, and RP031B-15AET), Malaysia's Fundamental Research Grant Scheme (FRGS/1/2015/SG05/UNIM/03/1), Prototype Research Grant Scheme (PRGS/2/2015/SG05/UNIM/03/1), Malaysia's Ministry of Science, Technology, Innovation (MOSTI 02-02-12-SF0256), Taiwan's Ministry of Science and Technology (106-3113-E-006 -011, 106-3113-E-006 -004CC2, and 103-2221-E-006-190-MY3) and Taiwan's Ministry of Education on Top University Grants.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017
Y1 - 2017
N2 - In this work, the extraction of microalgal protein from wet Chlorella sorokiniana species using alcohol/salt liquid biphasic flotation (LBF) with the aid of ultrasonication for cell rupturing was proposed. The effect of varying crude feedstock concentration, flotation time, salt type, salt concentration, alcohol type, alcohol concentration, initial volumes of salt and alcohol were investigated. After the optimization process, the highest proportion of protein recovered in the top phase was achieved with 250 g/L ammonium sulphate, 60% (v/v) 2-propanol, 1.0 VR,initial, 20 g/L crude biomass load, 4 mm3/min air flowrate and 10 min of flotation time. The recycling of phase components was introduced to minimize the use of alcohol and salt in the corresponding LBF. It was demonstrated that top phase (alcohol) recycling can achieve increasing performance for three consecutive recycling runs. Under optimized process conditions, the proportion of protein recovered in the top phase was 88.86% for the third recycle run.
AB - In this work, the extraction of microalgal protein from wet Chlorella sorokiniana species using alcohol/salt liquid biphasic flotation (LBF) with the aid of ultrasonication for cell rupturing was proposed. The effect of varying crude feedstock concentration, flotation time, salt type, salt concentration, alcohol type, alcohol concentration, initial volumes of salt and alcohol were investigated. After the optimization process, the highest proportion of protein recovered in the top phase was achieved with 250 g/L ammonium sulphate, 60% (v/v) 2-propanol, 1.0 VR,initial, 20 g/L crude biomass load, 4 mm3/min air flowrate and 10 min of flotation time. The recycling of phase components was introduced to minimize the use of alcohol and salt in the corresponding LBF. It was demonstrated that top phase (alcohol) recycling can achieve increasing performance for three consecutive recycling runs. Under optimized process conditions, the proportion of protein recovered in the top phase was 88.86% for the third recycle run.
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U2 - 10.1016/j.biortech.2017.05.165
DO - 10.1016/j.biortech.2017.05.165
M3 - Article
C2 - 28602664
AN - SCOPUS:85020309996
SN - 0960-8524
VL - 244
SP - 1329
EP - 1336
JO - Bioresource technology
JF - Bioresource technology
ER -