Proteins recovery from wet microalgae using liquid biphasic flotation (LBF)

Win Nee Phong, Pau Loke Show, Wei Heng Teh, Tiong Xin Teh, Hilary Mae Yan Lim, Nurul Shafira binti Nazri, Chung Hong Tan, Jo Shu Chang, Tau Chuan Ling

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

In this work, the extraction of microalgal protein from wet Chlorella sorokiniana species using alcohol/salt liquid biphasic flotation (LBF) with the aid of ultrasonication for cell rupturing was proposed. The effect of varying crude feedstock concentration, flotation time, salt type, salt concentration, alcohol type, alcohol concentration, initial volumes of salt and alcohol were investigated. After the optimization process, the highest proportion of protein recovered in the top phase was achieved with 250 g/L ammonium sulphate, 60% (v/v) 2-propanol, 1.0 VR,initial, 20 g/L crude biomass load, 4 mm3/min air flowrate and 10 min of flotation time. The recycling of phase components was introduced to minimize the use of alcohol and salt in the corresponding LBF. It was demonstrated that top phase (alcohol) recycling can achieve increasing performance for three consecutive recycling runs. Under optimized process conditions, the proportion of protein recovered in the top phase was 88.86% for the third recycle run.

Original languageEnglish
Pages (from-to)1329-1336
Number of pages8
JournalBioresource technology
Volume244
DOIs
Publication statusPublished - 2017

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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