Proteomic Analyses Reveal the Role of Progesterone and Inducible Nitric Oxide Synthase in Uterine Remodeling during Pregnancy

Chung Lin Wang, Shu-Hui Chen, Cheng Ping Wu, Chen-Hsiang Yu, Mei-Ling Tsai

Research output: Contribution to journalReview article

Abstract

In the luteal phase the uterus undergoes a series of changes in uterine proteins and creates a receptive window for embryo attachment. Invasion of implanted embryos into the endometrium initiates both morphological and functional remodeling of pregnant uteri with continuous elevation of progesterone. Rapid development of proteomic analysis offers additional approaches to construct the protein database of pregnant uteri for the study of embryo-induced remodeling. Comparative proteomic studies followed by functional annotation demonstrate the role of progesterone and its downstream molecule (Hoxa10) in regulating the expression of immmunosuppresive proteins (such as glycodelin) and chaperone proteins (such as FK506 binding protein 4). Further improvement in the technology of protein digestion and separation increases the number of myometrial proteins identified. Validation studies indicate a unique expression pattern and functions of inducible nitric oxide synthase during pregnancy. The combinatory effect of inducible nitric oxide synthase and progesterone contributes to decidual remodeling during pregnancy.

Original languageEnglish
Pages (from-to)301-308
Number of pages8
JournalJournal of Experimental and Clinical Medicine
Volume4
Issue number6
DOIs
Publication statusPublished - 2012 Dec 1

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Nitric Oxide Synthase Type II
Proteomics
Progesterone
Uterus
Pregnancy
Embryonic Structures
Proteins
Protein Databases
Validation Studies
Luteal Phase
Endometrium
Proteolysis
Technology

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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abstract = "In the luteal phase the uterus undergoes a series of changes in uterine proteins and creates a receptive window for embryo attachment. Invasion of implanted embryos into the endometrium initiates both morphological and functional remodeling of pregnant uteri with continuous elevation of progesterone. Rapid development of proteomic analysis offers additional approaches to construct the protein database of pregnant uteri for the study of embryo-induced remodeling. Comparative proteomic studies followed by functional annotation demonstrate the role of progesterone and its downstream molecule (Hoxa10) in regulating the expression of immmunosuppresive proteins (such as glycodelin) and chaperone proteins (such as FK506 binding protein 4). Further improvement in the technology of protein digestion and separation increases the number of myometrial proteins identified. Validation studies indicate a unique expression pattern and functions of inducible nitric oxide synthase during pregnancy. The combinatory effect of inducible nitric oxide synthase and progesterone contributes to decidual remodeling during pregnancy.",
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AU - Chen, Shu-Hui

AU - Wu, Cheng Ping

AU - Yu, Chen-Hsiang

AU - Tsai, Mei-Ling

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